A5084762425- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Retinal Development and Disorders
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Sleep and Wakefulness Research
- Biotin and Related Studies
- Neuroendocrine regulation and behavior
- Circadian rhythm and melatonin
- Connexins and lens biology
- Neurobiology and Insect Physiology Research
- Genetics and Neurodevelopmental Disorders
- Mitochondrial Function and Pathology
- Zebrafish Biomedical Research Applications
- Receptor Mechanisms and Signaling
- Physiological and biochemical adaptations
University of Zurich
2018-2024
ETH Zurich
2018-2024
University of Ottawa
2017-2022
Institute of Pharmacology
2019-2022
Institut des Sciences Cognitives Marc Jeannerod
2019
Abstract Neurons are highly compartmentalized cells with tightly controlled subcellular protein organization. While brain transcriptome, connectome and global proteome maps being generated, system-wide analysis of temporal dynamics at the level currently lacking. Here, we perform a temporally-resolved surfaceome primary neuron cultures reveal dynamic surface clusters that reflect functional requirements during distinct stages neuronal development. Direct comparison total pools development...
The precise function of specialized GABAergic interneuron subtypes is required to provide appropriate synaptic inhibition for regulating principal neuron excitability and synchronization within brain circuits. Of these, parvalbumin-type (PV neuron) dysfunction a feature several sex-biased psychiatric disorders, although, the underlying developmental mechanisms are unclear. While transcriptional action sex hormones generates sexual dimorphism during development, whether kinase signaling...
Abstract Sleep/wake cycles intricately shape physiological activities including cognitive brain functions, yet the precise molecular orchestrators of sleep remain elusive. Notably, clinical impact benzodiazepine drugs underscores pivotal role GABAergic neurotransmission in regulation. However, specific contributions distinct GABA A receptor subtypes and their principal scaffolding protein, gephyrin, dynamics unclear. The evolving synaptic phospho‐proteome alterations at excitatory inhibitory...
Abstract In dissociated neuronal cultures the absence of spatial and temporal cues causes emergence mismatched synapses, where post‐synaptic proteins GABA ergic synapses are in part apposed to glutamatergic pre‐synaptic terminals vice versa. This mismatch offers an opportunity study mechanisms that regulate correct apposition pre‐ elements. We report here IQ motif Sec7 domain‐containing protein 3 ( IQSEC 3; BRAG synArf GEF ) specifically regulates mislocalization density PSD proteins....
GABAergic inhibitory neurotransmission contributes to diverse aspects of brain development and adult plasticity, including the expression complex cognitive processes. This is afforded for in part by dynamic adaptations occurring at synapses, which show great heterogeneity both terms upstream signalling downstream effector mechanisms. Single-particle tracking live imaging have revealed that receptor-scaffold interactions critically determine synapses. Super-resolution studies shown protein...
Neuroscience currently requires the use of antibodies to study synaptic proteins, where antibody binding is used as a correlate define presence, plasticity, and regulation synapses. Gephyrin an inhibitory scaffolding protein mark GABAergic glycinergic postsynaptic sites. Despite importance gephyrin in modulating transmission, its limited by tractability available reagents. Designed Ankyrin Repeat Proteins (DARPins) are class synthetic binder derived from diverse libraries vitro selection...
Horizontal cells (HCs) are interneurons of the outer retina that undergo graded changes in membrane potential during light response and provide feedback to photoreceptors. We characterized spontaneous Ca2+-based action potentials (APs) isolated goldfish (Carassius auratus) HCs with electrophysiological intracellular imaging techniques. Transient Ca2+ concentration ([Ca2+]i) were observed fura-2 abolished by removal extracellular or inhibition channels 50 µM Cd2+ 100 nifedipine. Inhibition...
ABSTRACT Seasonal modifications in the structure of cellular membranes occur as an adaptive measure to withstand exposure prolonged environmental change. Little is known about whether such changes independently external cues, photoperiod or temperature, how they may impact central nervous system. We compared membrane properties neurons isolated from retina goldfish (Carassius auratus), organism well adapted extreme change, during summer and winter months. Goldfish were maintained a facility...
Searching for food follows a well-organized decision process in mammals to take up only if necessary. Moreover, scavenging is preferred during their activity phase. Various time-dependent regulatory processes have been identified originating from the suprachiasmatic nuclei (SCN), which convert external light information into synchronizing output signals. However, direct impact of SCN on timing normal searching has not yet found. Here, we revisited function affect when mice look food. We...
Neurons are highly compartmentalized cells with tightly controlled subcellular protein organization. While broad brain transcriptome, connectome and global proteome maps being generated, system-wide analysis of temporal dynamics at the level currently lacking for neuronal development synapse formation. We performed a temporally-resolved surfaceome developing primary neuron cultures to depth 1000 bona fide surface proteins reveal dynamic clusters that reflect functional requirements during...
ABSTRACT Seasonal modifications in the structure of cellular membranes occur as an adaptive measure to withstand exposure prolonged environmental change. Little is known about whether such changes may independently external cues, photoperiod or temperature, how they impact central nervous system (CNS). We compared membrane properties neurons isolated from retina goldfish ( Carassius auratus ), organism well-adapted extreme change, during summer and winter months. Goldfish were maintained a...
Abstract Neuroscience currently requires the use of antibodies to study synaptic proteins, where antibody binding is used as a correlate define presence, plasticity, and regulation synapses. Gephyrin an inhibitory scaffolding protein mark GABAergic glycinergic postsynaptic sites. Despite importance gephyrin in modulating transmission, its limited by tractability available reagents. Designed Ankyrin Repeat Proteins (DARPins) are class synthetic binder derived from diverse libraries vitro...