A5046163178- Advanced Proteomics Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Cellular transport and secretion
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Cell Image Analysis Techniques
- Neuroscience and Neuropharmacology Research
- Ion Channels and Receptors
- Immune Response and Inflammation
- Lipid Membrane Structure and Behavior
- Single-cell and spatial transcriptomics
- Microbial Inactivation Methods
- Acute Myeloid Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Nanoplatforms for cancer theranostics
- Biotin and Related Studies
- Advanced Biosensing Techniques and Applications
- Electromagnetic Fields and Biological Effects
- RNA and protein synthesis mechanisms
- Image Processing and 3D Reconstruction
- Ubiquitin and proteasome pathways
- Chronic Myeloid Leukemia Treatments
- Botulinum Toxin and Related Neurological Disorders
ETH Zurich
2018-2025
University of Basel
2025
Max Planck Institute for Brain Research
2020-2024
Institute of Translational Health Sciences
2022
SIB Swiss Institute of Bioinformatics
2019-2021
Department of Medical Sciences
2019
Institut des Sciences Cognitives Marc Jeannerod
2019
Cell-surface proteins are of great biomedical importance, as demonstrated by the fact that 66% approved human drugs listed in DrugBank database target a cell-surface protein. Despite this relevance, there has been no comprehensive assessment surfaceome, and only fraction predicted 5,000 transmembrane have shown to be located at plasma membrane. To enable analysis we developed surfaceome predictor SURFY, based on machine learning. As training set, used experimentally verified high-confidence...
Neurons build synaptic contacts using different protein combinations that define the specificity, function, and plasticity potential of synapses; however, diversity proteomes remains largely unexplored. We prepared synaptosomes from 7 transgenic mouse lines with fluorescently labeled presynaptic terminals. Combining microdissection 5 brain regions fluorescent-activated synaptosome sorting (FASS), we isolated analyzed 18 synapse types. discovered ∼1,800 unique synapse-type-enriched proteins...
Different cell isolation techniques exist for transcriptomic and proteotype profiling of brain cells. Here, we provide a systematic investigation the influence different protocols on transcriptional profiles in mouse tissue by taking into account single-cell transcriptomics cells, proteotypes microglia astrocytes, flow cytometric analysis microglia. We show that standard enzymatic digestion at 37 °C induces profound consistent alterations transcriptome neuronal glial as compared to an...
The molecular nanoscale organization of the surfaceome is a fundamental regulator cellular signaling in health and disease. Technologies for mapping spatial relationships cell surface receptors their extracellular synapses would unlock theranostic opportunities to target protein communities possibility engineer signaling. Here, we develop an optoproteomic technology termed LUX-MS that enables targeted elucidation acute interactions on between living cells using light-controlled singlet...
Abstract Neurons are highly compartmentalized cells with tightly controlled subcellular protein organization. While brain transcriptome, connectome and global proteome maps being generated, system-wide analysis of temporal dynamics at the level currently lacking. Here, we perform a temporally-resolved surfaceome primary neuron cultures reveal dynamic surface clusters that reflect functional requirements during distinct stages neuronal development. Direct comparison total pools development...
Abstract System-wide quantification of the cell surface proteotype and identification extracellular glycosylation sites is challenging when samples are limited. Here, we miniaturize automate previously described Cell Surface Capture (CSC) technology, increasing sensitivity, reproducibility throughput. We use this which call autoCSC, to create population-specific surfaceome maps developing mouse B cells targeted flow cytometry uncover developmental subpopulations.
Abstract Brain function relies on communication via neuronal synapses. Neurons build and diversify synaptic contacts using different protein combinations that define the specificity, plasticity potential of More than a thousand proteins have been globally identified in both pre- postsynaptic compartments, providing substantial for diversity. While there is ample evidence diverse structures, states or functional properties, diversity underlying individual proteomes remains largely unexplored....
Myelofibrosis is a hematopoietic stem cell disorder belonging to the myeloproliferative neoplasms. patients frequently carry driver mutations in either JAK2 or Calreticulin (CALR) and have limited therapeutic options. Here, we integrate ex vivo drug response proteotype analyses across myelofibrosis patient cohorts discover targetable vulnerabilities associated strategies. Drug sensitivities of mutated progenitor cells were measured blood using high-content imaging single-cell deep...
Abstract Proteins that interact together participate in the same cellular process and influence organismal traits. Despite progress mapping protein-protein interactions we lack knowledge of how they differ between tissues. Due to coordinated (post)transcriptional control, protein complex members have highly correlated abundances are predictive functional association. Here, compiled 7873 proteomic samples measuring levels 11 human tissues use these define an atlas with tissue-specific...
Abstract Despite progress in mapping protein–protein interactions, their tissue specificity is understudied. Here, given that protein coabundance predictive of functional association, we compiled and analyzed abundance data 7,811 proteomic samples from 11 human tissues to produce an atlas tissue-specific associations. We find this method recapitulates known complexes the larger structural organization cell. Interactions stable are well preserved across tissues, while cell-type-specific...
Exposure of the developing brain to immune mediators, including antibodies, is postulated increase risk for neurodevelopmental disorders and neurodegenerative disease. It has been suggested that immunoglobulin G-immune complexes (IgG-IC) activate Fc gamma receptors (FcγR) expressed on neurons modify signaling events in these cells. However, testing this hypothesis hindered by a paucity data regarding neuronal FcγR expression function.FcγR transcript hippocampus, cortex, cerebellum neonatal...
Proteostasis - the maintenance of proteins at proper concentrations, conformations, and subcellular locations is essential for cellular function governed by tightly regulated protein synthesis degradation pathways. The Proteasome Maturation Protein (POMP) a key chaperone involved in assembling proteasome, primary complex responsible degradation. Despite conserved role POMP, its loss produces contrasting proteostatic effects yeast mammalian cells, pointing to additional, unexplored functions....
Abstract Different cell isolation techniques exist for transcriptomic and proteotype profiling of brain cells. Here, we provide a systematic investigation the influence different protocols on transcriptional profiles in mouse tissue by taking into account single-cell transcriptomics cells, proteotypes microglia astrocytes, flow cytometric analysis microglia. We show that standard enzymatic digestion at 37°C induces profound consistent alterations transcriptome neuronal glial as compared to...
Pulsed electromagnetic fields (PEMFs) are capable of specifically activating a TRPC1-mitochondrial axis underlying cell expansion and mitohormetic survival adaptations. This study characterizes cell-derived vesicles (CDVs) generated from C2C12 murine myoblasts shows that they equipped with the sufficient molecular machinery to confer mitochondrial respiratory capacity associated proliferative responses upon their fusion recipient cells. CDVs derived wild type include cation-permeable...
Herein we report an<italic>in vitro</italic>approach to selectively quantify physiologically active BoNT/A only. This technology is based on the use of nerve cell-mimicking nanoreactors in combination with microfluidics.
Abstract System-wide quantification of the cell surface proteotype and identification extracellular glycosylation sites is challenging when sample limiting. We miniaturized automated previously described Cell Surface Capture technology increasing sensitivity, reproducibility, throughput. used this technology, which we call autoCSC, to create population-specific surfaceome maps developing mouse B cells targeted flow cytometry uncover developmental subpopulations.
Abstract Pulsed electromagnetic fields (PEMFs) are capable of specifically activating a TRPC1-mitochondrial axis underlying cell expansion and mitohormetic survival adaptations. This study characterizes cell-derived vesicles (CDVs) generated from C2C12 murine myoblasts shows that they equipped with the sufficient molecular machinery to confer mitochondrial respiratory capacity associated proliferative responses upon their fusion recipient cells. CDVs derived wild type include...
Abstract Delineating the molecular nanoscale organization of surfaceome is pre-requisite for understanding cellular signaling. Technologies mapping spatial relationships cell surface receptors and their extracellular signaling synapses would open up theranostic opportunities possibility to engineer Here, we developed an optoproteomic technology termed LUX-MS that exploits singlet oxygen generators (SOG) light-triggered identification acute protein interactions on living cells. Using...
Neurons are highly compartmentalized cells with tightly controlled subcellular protein organization. While broad brain transcriptome, connectome and global proteome maps being generated, system-wide analysis of temporal dynamics at the level currently lacking for neuronal development synapse formation. We performed a temporally-resolved surfaceome developing primary neuron cultures to depth 1000 bona fide surface proteins reveal dynamic clusters that reflect functional requirements during...