A5080379855- Cancer-related Molecular Pathways
- 14-3-3 protein interactions
- Ubiquitin and proteasome pathways
- Molecular Biology Techniques and Applications
- Effects of Radiation Exposure
- Lung Cancer Treatments and Mutations
- Cancer Cells and Metastasis
- Cervical Cancer and HPV Research
- Radiation Therapy and Dosimetry
- Advanced Radiotherapy Techniques
- Cancer, Hypoxia, and Metabolism
- Esophageal Cancer Research and Treatment
- Gene expression and cancer classification
- ATP Synthase and ATPases Research
- RNA Research and Splicing
- TGF-β signaling in diseases
- Melanoma and MAPK Pathways
- Single-cell and spatial transcriptomics
- PI3K/AKT/mTOR signaling in cancer
- Radiation Detection and Scintillator Technologies
- Chemotherapy-induced cardiotoxicity and mitigation
- Lung Cancer Research Studies
- Genetic factors in colorectal cancer
- Ultrasound and Hyperthermia Applications
- Forensic and Genetic Research
Isala
2021-2024
University Medical Center Groningen
2007-2018
University of Groningen
2010-2013
Leiden University
2004-2008
Abstract Precision cancer medicine has changed the treatment paradigm of patients with non-small cell lung (NSCLC) specific molecular aberrations. A major challenge is management resistance that tumor cells eventually develop against targeted therapies, either through primary or acquired mechanisms. We report a 61 year-old male patient metastatic NSCLC harboring an EGFR exon 19 deletion, PIK3CA mutation, and CDK4 amplification. After initial partial response to osimertinib as mono-therapy...
Nucleocytoplasmic transport of proteins plays an important role in the regulation many cellular processes. Differences nucleocytoplasmic shuttling can provide a basis for isoform-specific biological functions members multigene families, like 14-3-3 protein family. Many organisms contain multiple isoforms, which play numerous processes, including signalling, cell cycle control and apoptosis. It is still unclear whether these isoforms have specialised this specialisation based on ligand...
The p53 homolog p73 is frequently overexpressed in cancers. Especially the transactivation domain truncated isoform ΔNp73 has oncogenic properties and its upregulation associated with poor patient survival. It been shown that an inhibitory effect on capacity of other isoforms. Here, we confirm this finding but surprisingly find may also stimulate expression TGF-β signaling targets. Promoter-reporter analysis indicated presence Smad Binding Elements (SBE) promoter sufficient for stimulation...
Purpose: Compared to photons, using particle radiation in radiotherapy reduces the dose and irradiated volume of normal tissues, potentially reducing side effects. The biological effect deposited by particles such as carbon ions, however, differs from that photons. inaccuracy models estimate effects remains most important source uncertainties therapy. Improving this requires high‐precision studies on radiation. Therefore, authors aimed develop a facility for reproducible carbon‐ion...
Abstract Background Quick and reliable testing of EGFR KRAS is needed in non-small cell lung cancer ( NSCLC) to ensure optimal decision-making for targeted therapy. The Idylla™ platform was designed Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections but recently several studies were published that evaluated its potential cytological specimens. This study aimed validate the detection EGFR/KRAS mutations NSCLC samples prepared as cytoblocks using AGAR paraffin embedding. Material methods...
<title>Abstract</title> Disease diagnosis, prognosis, and therapy choice progressively rely on good-quality DNA for molecular analysis. As tissue processing is routinely performed worldwide with ancient techniques using toxic DNA-damaging formaldehyde, the quality of isolated from embedded tissues used diagnostics poor. We a novel formalin-free method to process show that, after 5 years, superior formalin fixation.
Disease diagnosis, prognosis, and therapy choice progressively rely on good-quality Deoxyribonucleic acid (DNA) for molecular analysis. As tissue processing is routinely performed worldwide with ancient techniques using toxic DNA-damaging formaldehyde, the quality of DNA isolated from embedded tissues used diagnostics poor. We a novel formalin-free embedding method to process show that, after 5 years, superior formalin fixation.
36 Background: Neo-adjuvant chemoradiation is considered as standard treatment in esophageal cancer (EC). However, a significant proportion of these tumors do not respond well to radiotherapy. Here, we investigated the presence radioresistant cells EC with stem cell characteristics. Methods: OE-33 adenotype and OE-21 squamous carcinoma cell-lines fresh tumor material patients were analyzed for common CSC-markers Fluorecence Activated Cell Sorting (FACS) immunohistochemistry. Subpopulations...