A5043919064- Prostate Cancer Treatment and Research
- Bone health and treatments
- Radiopharmaceutical Chemistry and Applications
- Cancer Cells and Metastasis
- Wnt/β-catenin signaling in development and cancer
- Cell Adhesion Molecules Research
- Cancer Research and Treatments
- Immune Response and Inflammation
- Global Cancer Incidence and Screening
- HER2/EGFR in Cancer Research
- Bone Metabolism and Diseases
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related gene regulation
- 3D Printing in Biomedical Research
- Virus-based gene therapy research
- Cellular Mechanics and Interactions
- Breast Cancer Treatment Studies
- Fatty Acid Research and Health
- Cancer Genomics and Diagnostics
- Health disparities and outcomes
- Cancer-related Molecular Pathways
- Mechanisms of cancer metastasis
- Clostridium difficile and Clostridium perfringens research
- Neurological Disease Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
The University of Texas Health Science Center at Houston
2025
University of Massachusetts Amherst
2016-2024
University of California, Los Angeles
2020
Public Health Agency
2011-2019
The University of Texas MD Anderson Cancer Center
2003-2019
University of Colorado Denver
2019
Florida Southern College
2018
F-star (United Kingdom)
2018
St George's, University of London
2018
In-Q-Tel
2017
Although current breast cancer treatment guidelines limit the use of HER2-blocking agents to tumors with HER2 gene amplification, recent retrospective analyses suggest that a wider group patients may benefit from this therapy. Using cell lines, mouse xenograft models and matched human primary metastatic tissues, we show is selectively expressed in regulates self-renewal stem (CSC) population estrogen receptor-positive (ER(+)), HER2(-) luminal cancers. trastuzumab had no effects on growth...
Abstract Critical cancer pathways often cannot be targeted because of limited efficiency crossing cell membranes. Here we report the development a Salmonella-based intracellular delivery system to address this challenge. We engineer genetic circuits that (1) activate regulator flhDC drive invasion and (2) induce lysis release proteins into tumor cells. Released protein drugs diffuse from Salmonella containing vacuoles cellular cytoplasm where they interact with their therapeutic targets....
Abstract Prostate cancer produces painful osteoblastic bone metastases. Although prostate cells produce numerous osteogenic factors, to date, none have been shown mediate metastases in an vivo model of cancer. Wnts are a large family proteins that promote growth. Wnt activity is antagonized by endogenous including dickkopf-1 (DKK-1). We explored if through using DKK-1 as tool modify activity. A variety mRNAs were found be expressed cell lines and mRNA expression was increased primary...
Activation of the EGF receptors EGFR (ErbB1) and HER2 (ErbB2) drives progression multiple cancer types through complex mechanisms that are still not fully understood. In this study, we report expression is elevated in bone metastases prostate independently gene amplification. An examination NF-κB receptor (RANK) coexpression revealed increased levels both proteins aggressive tumors metastatic deposits. Inhibiting tumor xenografts reduced proliferation RANK while maintaining expression....
Abstract Prostate cancer (PCa) is frequently accompanied by osteosclerotic (i.e., excessive bone production) metastases. Although morphogenetic proteins (BMP) and Wnts are mediators of PCa-induced osteoblastic activity, the relation between them in PCa metastases unknown. The goal this study was to define relationship. Wnt3a Wnt5a administration or knockdown DKK-1, a Wnt inhibitor, induced BMP-4 6 expression promoter activation cells. DKK-1 blocked BMP promoters. Transfection C4-2B cells...
Barney et al. used biomaterials to uncover a role for fibronectin in preventing breast cancer cell escape from dormancy.
Abstract BACKGROUND Prostate cancer (PCa) frequently metastasizes to the bone and induces osteoblastic lesions. We previously demonstrated through over‐expression of Wnt inhibitor dickkopf‐1 (DKK‐1) that Wnts contribute component PCa osseous lesions in vivo. METHODS To test clinical significance DKK‐1 expression during progression, tissue microarrays were stained for protein by immunohistochemistry. RESULTS index (EI) was found increase PIN primary compared non‐neoplastic (106 ± 10 vs. 19 6,...
Abstract The most frequent site of prostate cancer metastasis is the bone. Adhesion to bone-specific factors may facilitate selective skeleton. Therefore, we tested whether bone mediated by binding type I collagen, abundant protein. We observed that only metastatic cells bound collagen I, whereas form visceral metastases failed bind collagen. To confirm relationship between adhesion and potential, a collagen-binding variant human LNCaP was derived through serial passage on (LNCaPcol)....
Enterobacteriaceae cells growing in liquid media shed fragments of their outer membranes. These fragments, which may constitute a biologically important form gram-negative bacterial endotoxin, have been reported to contain proteins, phospholipids, and lipopolysaccharides (LPS). In this study we compared the sizes LPS molecules membrane membranes from broth cultures. Using conditional mutants Salmonella typhimurium incorporate specific sugars into LPS, analyzed radiolabeled by sodium dodecyl...
The most frequent site of metastasis in human prostate cancer (PCa) is the bone. Preferential adhesion PCa cells to bone-specific factors may facilitate selective skeleton. abundant protein within skeleton type I collagen. We previously demonstrated that selected vitro for collagen binding (LNCaP(col)) are highly motile and acquired capacity grow bone compared nontumorigenic LNCaP parental cells. Treatment with alpha(2)beta(1)-neutralizing antibodies selectively blocked collagen-stimulated...
Abstract Most breast cancer metastases in bone form osteolytic lesions, but the mechanisms of tumor‐induced resorption and destruction are not fully understood. Although it is well recognized that Wnt/β‐catenin signaling important for tumorigenesis, role this pathway metastasis unclear. Dickkopf1 (Dkk1) a secreted antagonist. In present study, we demonstrated activation enhanced Dkk1 expression cells overexpression frequent event cancer. We also found human cell lines preferentially...
These studies were undertaken to investigate the binding of gram-negative bacterial lipopolysaccharides (LPS) high-density lipoproteins (HDL) rat plasma. Purified Salmonella typhimurium LPS, intrinsically labeled with [3H]-galactose, bound rapidly in vitro isolated HDL. Maximal LPS HDL occurred when and incubated lipoprotein-free plasma (rho greater than 1.21 g/ml). Since purified, form large aggregates, we tested hypothesis that disaggregation enhances LPS-HDL binding. We found calcium...
Improved in vitro models are needed to better understand cancer progression and bridge the gap between proof-of-concept studies, vivo validation, clinical application. Multicellular tumor spheroids (MCTS) a popular method for three-dimensional (3D) cell culture, because they capture some aspects of dimensionality, cell-cell contact, cell-matrix interactions seen vivo. Many approaches exist create MCTS from lines, have been used study invasion, growth, how cells respond drugs physiologically...
Lipid A, the toxic moiety of Gram-negative bacterial lipopolysaccharides (endotoxins), is a glucosamine disaccharide to which fatty acid and phosphate residues are covalently attached. Recent studies Salmonella lipid A indicate that 3-hydroxytetradecanoic (3-OH-14:0) directly linked backbone nonhydroxylated acids (principally dodecanoic tetradecanoic acids) esterified hydroxyl groups some 3-OH-14:0 molecules. We report here granule fraction human neutrophils contains one or more enzymes...
Macrophages are thought to play a central role in the responses of animals gram-negative bacterial lipopolysaccharides (LPS). Since nothing is known about metabolism LPS by these cells, we studied uptake and deacylation radiolabeled thioglycolate-elicited peritoneal macrophages from normal (C3H/HeN) LPS-hyporesponsive (C3H/HeJ) mice. both kinds mice took up deacylated that were added culture medium. Opsonization with anti-LPS immunoglobulin G antibodies greatly increased uptake; opsonized...
ABSTRACT The diarrheal pathogen Clostridium difficile consists of at least six distinct evolutionary lineages. RT017 lineage is anomalous, as strains only express toxin B, compared to from other lineages that produce toxins A and B and, occasionally, binary toxin. Historically, initially was reported in Asia but now has been worldwide. We used whole-genome sequencing phylogenetic analysis investigate the patterns global spread population structure 277 isolates animal human origins...
Traditional drug screening methods lack features of the tumor microenvironment that contribute to resistance. Most studies examine cell response in a single biomaterial platform depth, leaving gap understanding how extracellular signals such as stiffness, dimensionality, and cell–cell contacts act independently or are integrated within affect either sensitivity This is critically important, adaptive resistance mediated, at least part, by matrix (ECM) microenvironment. We developed an...
Leukocytes contain an enzyme that detoxifies bacterial lipopolysaccharides (also called endotoxins) by removing fatty acyl chains are attached in acyloxy linkage to the glucosamine backbone of lipid A. We describe purification carries out this activity, termed acyloxyacyl hydrolysis, from HL-60 human promyelocyte cell line. The is a glycoprotein apparent Mr = 52,000-60,000 found low abundance (less than 0.001% lysate protein), principally granule fraction cells. protein has two...