A5086000808- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Pregnancy and preeclampsia studies
- Cytokine Signaling Pathways and Interactions
- Endometrial and Cervical Cancer Treatments
- Estrogen and related hormone effects
- Reproductive Physiology in Livestock
- MicroRNA in disease regulation
- Reproductive Biology and Fertility
- Galectins and Cancer Biology
- Birth, Development, and Health
- Gynecological conditions and treatments
- Pregnancy-related medical research
- Ectopic Pregnancy Diagnosis and Management
- Angiogenesis and VEGF in Cancer
- Gestational Trophoblastic Disease Studies
- Bone health and treatments
- Fibroblast Growth Factor Research
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Preterm Birth and Chorioamnionitis
- Cell Adhesion Molecules Research
- Pregnancy and Medication Impact
- Growth Hormone and Insulin-like Growth Factors
- Prenatal Screening and Diagnostics
- TGF-β signaling in diseases
The University of Melbourne
1991-2024
Royal Women's Hospital
2019-2024
Monash University
2013-2023
Hudson Institute of Medical Research
2014-2023
St Vincents Institute of Medical Research
2004-2021
Monash Institute of Medical Research
2005-2014
Monash Medical Centre
2007-2012
Institute for Medical Research
2003-2011
University of California, Davis
2010
National Institutes of Health
2010
Preimplantation cross-talk between a functional blastocyst and the endometrium is critical for successful implantation. This interaction mediated in part by endometrial cytokines/growth factors secreted glandular epithelium into uterine cavity. Recent evidence suggests that blastocyst-derived hCG may influence milieu conception cycles thereby enhancing receptivity implantation success. study investigated effect of on secretory profile select cohort 44 from primary human epithelial cells...
The human endometrium is receptive for implantation of a blastocyst only 4–5 days in each menstrual cycle. Failure major reason infertility women and the inability to achieve endometrial receptivity responsible much failure reproductive technologies. Endometrial requires changes uterine luminal glandular cells, particularly terms their secretory capacity altered expression adhesion molecules. In parallel with these changes, decidualisation (differentiation) stroma initiated during phase,...
Differentiation of endometrial stromal cells into decidual is crucial for embryo implantation and placentation. Interleukin (IL)-11 signalling essential adequate decidualization in the mouse uterus. We examined role IL-11 during progesterone-induced human over a 10–12 day period, using prolactin (PRL) production as marker. These produced biologically active expressed IL-11, IL-11Rα PRL mRNA decidualization. Neutralization endogenous with an anti-human (hu)IL-11 antibody (AB) reduced from 8...
Embryo implantation requires the closely harmonized processes of apposition, attachment, and adhesion conceptus to maternal endometrial epithelium. IL-11 leukemia inhibitory factor (LIF), two IL-6 family cytokines, are produced by endometrium absolutely required for in mice. We examined effect LIF on human epithelial cell adhesion. Both cytokines increased primary cells fibronectin collagen IV. stimulated, whereas had no trophoblast cells. Focused oligogene arrays were used identify...
Adequate differentiation or decidualization of endometrial stromal cells (ESC) is critical for successful pregnancy in humans and rodents. Here, we investigated the role leukemia inhibitory factor (LIF) human murine decidualization. Ex vivo (H) ESC was induced by estrogen (E, 10−8 M) plus medroxyprogesterone acetate (MPA, 10−7 M). Exogenous LIF (≥50 ng/ml) STAT3 phosphorylation non-decidualized decidualized HESC enhanced E+MPA-induced (measured PRL secretion, P<0.05). mRNA down-regulated...
Successful blastocyst implantation requires the differentiation of human endometrial stromal cells (HESC), a process known as decidualization. Activin A, transforming growth factor beta (TGFbeta) superfamily member, enhances HESC decidualization and localizes to decidual in endometrium. Other TGFbeta members, including BMP2, BMP4, BMP7, GDF5, GDF8, GDF11, TGFbetas Nodal, may also play role during This study aimed identify these family members endometrium, determine whether they are involved...
Significance Preeclampsia is an insidious disease, unique to humans, affecting ∼8% of pregnancies. There are no early detection tests or pharmacological treatments. Impaired placentation widely accepted contribute the pathogenesis. However, mechanisms remain elusive, given complications studying first-trimester placental development in women. A major limitation for study new treatments lack available animal models that recapitulate full spectrum preeclampsia features. We have developed a...
Interleukin-11 (IL-11) is crucial in the decidualization response of uterine stroma to implanting blastocyst mouse. This study examined localization and expression IL-11 human endometrium throughout menstrual cycle prolactin leukaemia inhibitory factor (LIF) secretory phase endometrium. The mRNA receptor α signalling component, gp130, endometrial tissue were also determined. Immunoreactive was highest present decidualized stromal cells, glandular epithelial endothelial smooth muscle verified...
Blastocyst implantation is a critical stage in the establishment of pregnancy. Leukemia inhibitory factor (LIF) essential for mouse blastocyst and also plays role human We examined effect potent LIF antagonist (LA) on implantation. In mice, expression peaks day 3.5 pregnancy (D3.5) (D0.5 = mating plug detection) uterine glandular epithelium. LA (7 mg/kg per day) administered from D2.5 to D4.5 via four hourly i.p. injections plus continuous administration miniosmotic pump resulted complete...
Leukemia inhibitory factor (LIF) is a pleiotropic cytokine that essential for blastocyst implantation in mice. It has been suggested LIF may play role human first trimester extravillous trophoblast (EVT) invasion. The aim of the present study was to establish whether induces changes EVT function related invasiveness. Primary cell cultures were treated with/without and effects on adhesion fibronectin (FN), vitronectin (VN) laminin (LN) assessed. Transcript levels integrin subunits mediate...
Trophoblast growth and invasion of the uterine endometrium are critical events during placentation tightly regulated by factors produced within trophoblast-endometrial microenvironment. Deficiencies in can result early miscarriage or preeclampsia intrauterine restriction, leading to impaired fetal health. The latter has been linked major adult health disorders. IL-11 is essential for blastocyst implantation mice. In humans, its receptor alpha (IL-11Ralpha) maximally expressed decidua...
Endometrial carcinoma is the most common gynaecological malignancy. There however a lack of curative therapies, especially for patients diagnosed with late stage, recurrent or aggressive disease, who have poor prognosis. Interleukin (IL) 11 pleiotropic cytokine that has role in number cancers including colon and breast cancer. IL11 was recently found to be upregulated endometrial cancers, function cancer not known. This study aimed determine effects on cell proliferation, adhesion migration....