A5052813352- Aquaculture disease management and microbiota
- interferon and immune responses
- Immune Response and Inflammation
- Invertebrate Immune Response Mechanisms
- Immune Cell Function and Interaction
- Antimicrobial Peptides and Activities
- Cytokine Signaling Pathways and Interactions
- T-cell and B-cell Immunology
- Viral Infections and Vectors
- Cancer-related molecular mechanisms research
- Aquaculture Nutrition and Growth
- Chemokine receptors and signaling
- MicroRNA in disease regulation
- Animal Genetics and Reproduction
- RNA regulation and disease
- Animal Virus Infections Studies
- Immunotherapy and Immune Responses
- Cell death mechanisms and regulation
- Cellular transport and secretion
- Physiological and biochemical adaptations
- RNA Research and Splicing
- Zebrafish Biomedical Research Applications
- Cell Adhesion Molecules Research
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
Shanghai Ocean University
2018-2025
Qingdao National Laboratory for Marine Science and Technology
2019-2025
Jiangxi Maternal and Child Health Hospital
2024-2025
National University of Singapore
2025
Ministry of Science
2025
Hunan Agricultural University
2019-2024
Guizhou University
2020-2022
Hunan Normal University
2022
Guangdong Pharmaceutical University
2021
Hunan Provincial People's Hospital
2021
Abstract IFN-γ is one of the key cytokines in defining Th1 immune responses. In this study, an homologue has been identified rainbow trout Oncorhynchus mykiss, and its biological activities have characterized. The cDNA 1034 bp length translates into a 180-aa protein. first intron gene contains highly polymorphic GACA minisatellites 44-bp DNA repeats, giving rise to at least six alleles. structurally conserved among vertebrates, signature motif identified. A nuclear localization sequence...
Abstract In this report, three type I IFN genes were identified in rainbow trout (rt) Oncorhynchus mykiss and are classified into two groups based on their primary protein sequences: group containing cysteine residues; II four cysteines residues. The rtIFNs induced fibroblasts (RTG-2 cells), macrophages (RTS-11 head kidney leukocytes when stimulated with polyinosinic:polycytidylic acid, whereas was up-regulated but not RTG-2 RTS-11 cells. Recombinant potent at inducing Mx expression...
A rainbow trout ( Oncorhynchus mykiss ) gene for tumor necrosis factor (TNF) has been cloned and sequenced. The cDNA contains an open reading frame of 738 nucleotides that translate into a 246 amino‐acid putative peptide, with 5′ untranslated region (UTR) 140 bp 3′ UTR 506 bp. Two potential N‐linked glycosylation sites exist in the translation. genomic sequence measures 2007 three introns intercept four coding exons. Expression studies using RT‐PCR have shown TNF is constitutively expressed...
The DExD/H domain containing RNA helicases such as retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated 5 (MDA5) are key cytosolic pattern recognition receptors (PRRs) for detecting nucleotide pathogen associated molecular patterns (PAMPs) of invading viruses. RIG-I MDA5 proteins differentially recognise conserved PAMPs in double stranded or single viral molecules, leading to activation the interferon system vertebrates. They share three core protein domains...
The retinoic acid-inducible gene I (RIG-I)-like receptors (RLR) comprise three homologues: RIG-I, melanoma differentiation-associated 5 (MDA5), and laboratory of genetics physiology 2 (LGP2). They activate the host interferon (IFN) system upon recognition viral RNA pathogen-associated molecular patterns (PAMPs) in cytoplasm. Bioinformatic analysis sequenced vertebrate genomes suggests that cytosolic surveillance is conserved lower vertebrates, recent functional studies have confirmed RIG-I...
Further to the previous finding of rainbow trout rtCATH_1 gene, this paper describes three more cathelicidin genes found in salmonids: two Atlantic salmon, named asCATH_1 and asCATH_2, one trout, rtCATH_2. All new salmonid share common characteristics mammalian genes, such as consisting four exons possessing a highly conserved preproregion invariant cysteines clustered C-terminal region cathelin-like domain. The gene is homologous that it possesses repeat motifs TGGGGGTGGC exon IV cysteine...
Abstract Interferons ( IFN s) have been reported in vertebrates from fish to mammals. To date, two types of s, i.e. type I and II s a range species aquaculture. In fish, are clarified into group , with cysteines four cysteines, further subgroups a, d, e, h, b, c, f, respectively. Group appear exist all species, whereas discovered only certain such as cyprinids, salmonids perciform fish. It seems preferentially functional that signal through cytokine receptor family B CRFB ) 5 1, while 2. But...
We investigated the antiviral activity and gene induction properties of interferon gamma (IFN-γ) compared to type I IFN (IFNa1) in Atlantic salmon. IFN-γ protected salmon cells against infectious pancreatic necrosis virus (IPNV)-induced cytopathic effect (CPE), reduced titers, inhibited synthesis viral structural protein VP3. Moreover, showed potent salmonid alphavirus 3 (SAV3) measured as a reduction nsP1 transcripts. (a II IFN) had less specific IPNV than IFNa1, showing half-maximal...
Abstract Fish type I IFNs are classified into two groups with (group I) or four II) cysteines in the mature peptide and can be further divided subgroups, termed IFN-a, -b, -c, -d. Salmonids possess all whereas other teleost species have one more but not groups. In this study, we discovered subgroups (IFN-e -f) rainbow trout Oncorhynchus mykiss analyzed expression of six brown Salmo trutta. RTG-2 RTS-11 cells, polyinosinic-polycytidylic acid stimulation resulted early activation IFN-d, IFN-e...
Abstract Heterogeneous nuclear ribonucleoproteins (hnRNPs) are a group of 34–120 kDa proteins that have recently been reported to participate in virus replication. The hnRNP family contains approximately 20 members, including A1, A2, A2B1, hnRNPC, hnRNPD and hnRNPK. HnRNPC plays important roles RNA biology, expression, stability, mRNA splicing, nonspecific sequence export 3’-end processing; however, the mechanisms underlying hnRNPC regulatory not fully understood. Here, we found zebrafish...
The role of the cytoplasmic regions interleukin-12 receptors (IL-12R) β1 and β2 in stimulating proliferation was examined. transmembrane IL-12Rβ1 or IL-12Rβ2 were fused to extracellular domain epidermal growth factor (EGF) receptor, yielding chimeric E12R1 E12R2, respectively. These chimeras stably transfected into BaF3 cells, a factor-dependent murine pro-B cell line. Only E12R2 E12R1+E12R2 transfectants capable EGF-dependent proliferation. phosphorylation JAK2, Tyk2, STAT3 observed. JAK2...
ABSTRACT We report the cloning of a novel antimicrobial peptide gene, termed rt CATH_1, found in rainbow trout, Oncorhynchus mykiss . The predicted 216-residue CATH_1 prepropeptide consists three domains: 22-residue signal peptide, 128-residue cathelin-like region containing two identifiable cathelicidin family signatures, and 66-residue C-terminal cationic peptide. This mature was unique possessing features different known (mammalian) subgroups, such as cysteine-bridged specific...