The pattern and intensity of glucocorticoid receptor (GR) heat shock 70 protein (Hsp 70) changes in the hippocampus brain cortex adult Wistar rat males exposed to acute (immobilization, cold) chronic (social isolation, crowding, daily swimming) stress or their combinations were followed by Western immunoblotting. Plasma ACTH CORT measured chemiluminescent method RIA. A significant decrease cytosol GR Hsp was observed after stress, while stresses led negligible both these proteins caused a...

The study deals with activity of three antioxidant enzymes, copper, zinc-superoxide dismutase (CuZnSOD), manganese superoxide (MnSOD), catalase (CAT) in hippocampus rats, following the exposure to single chronic (individual housing or forced swimming) and acute (immobilization cold) stress, as well combined chronic/acute stress. In addition, plasma noradrenaline (NA) adrenaline (A) concentrations were measured same stress conditions, because their autooxidation can add oxidative We observed...

The celiac disease is an autoimmune gastrointestinal disorder caused by gluten from wheat, rye or barley. In genetically predisposed persons, induces the immune-mediated inflammation of small intestinal mucosa. Histological lesions include intraepithelial lymphocytosis, crypt hypertrophy and villous atrophy, resulting in malabsorption micro- macronutrients. only treatment for patients a permanent gluten-free diet (GFD). Reactive oxygen species (ROS) oxidative stress are strongly associated...

This study examined the effects of lithium on gene expression and activity antioxidant enzymes copper zinc superoxide dismutase (SOD1), manganese (SOD2), catalase (CAT), glutathione peroxidase (GPx), reductase (GR) in hippocampus chronically stressed rats. In addition, we anxiety behaviors, hippocampal concentrations dopamine (DA) malondialdehyde (MDA), protein levels brain-derived neurotrophic factor (BDNF), tyrosine hydroxylase (TH), transporter (DAT), catechol-O-methyltransferase (COMT),...

Both the peripheral sympatho-adrenomedullary and central catecholaminergic systems are activated by various psycho-social physical stressors. Catecholamine stores in hypothalamus, hippocampus, adrenal glands, heart auricles of long-term socially isolated (21 days) control 3-month-old male Wistar rats, as well their response to immobilization all 4 limbs head fixed for 2 h cold stress (4ºC, h), were studied. A simultaneous single isotope radioenzymatic assay based on conversion catecholamines...

<i>Objective:</i> Social isolation is regarded as one of the most relevant causes diseases in mammalian species. The activation sympathoneural system represents key components stress response. sympathetic nervous major pathways involved immune-neuroendocrine interactions. aim present study was to determine plasma epinephrine and norepinephrine individually housed rats, well find out whether splenic gene expression catecholamine synthesizing enzymes their protein levels are...

The present study analyzed the effects of chronic treatment with low doses diazepam on body weight, defecations and urinations, vertical rears, elevated platform test, self-grooming in male rats exposed for 21 days to social isolation. were treated (0.2 mg/kg, i.p) or its vehicle. Social isolation led decreased weight more increased reluctance step down from test platform, shorter duration grooming, longer start grooming. Chronic individually housed produced increase decrease number...

1 Repeated maprotiline (a noradrenaline reuptake inhibitor) and fluoxetine serotonin treatment on gene expression of catecholamine biosynthetic enzymes were examined in adrenal medulla unstressed control chronic unpredictable mild stressed rats. 2 Maprotiline did not change 3 Fluoxetine increased tyrosine hydroxylase (TH) dopamine-β-hydroxylase (DBH), but phenylethanolamine N-methyltransferase both animals. 4 In conclusion, we have demonstrated that repeated administration enhanced...

ABSTRACT Chronic isolation of adult animals represents a form psychological stress that produces sympatho-adrenomedullar activation. Exercise training acts as an important modulator sympatho-adrenomedullary system. This study aimed to investigate physical exercise-related changes in gene expression catecholamine biosynthetic enzymes (tyrosine hydroxylase, dopamine-ß-hydroxylase and phenylethanolamine N-methyltransferase) cyclic adenosine monophosphate response element-binding (CREB) the...

Uterine leiomyomas are benign soft-tissues tumors that arise from uterine smooth muscle tissue. Etiopathogenesis of is not well understood. We aimed to examine whether antioxidant enzyme activities and lipid hydroperoxides level in patients with leiomyoma influenced by changes sex hormones gonadotropins (estradiol (E2), progesterone, FSH, LH) during menstrual cycle postmenopause. The material consisted blood tissue specimens. Hormone concentrations were determined assays for superoxide...

Abstract : Sensitivity of target cells to glucocorticoids is regulated by the expression intracellular glucocorticoid receptor (GR), which mediates effects glucocorticoids. The level GR and its nuclear transporter protein 70 (Hsp70) were followed in hippocampus brain cortex adult Wistar rat males exposed acute (immobilization, cold) chronic (social isolation, 15 min daily swimming) stress or their combinations. Changes plasma levels adenocorticotropic hormone corticosterone also studied. A...

The aim of the present study was to define stress-induced pattern cytosolic glucocorticoid receptor (GR) and Hsp70 protein in liver male Wistar rats exposed different stress models: acute (2 h/day) immobilization or cold (4 degrees C); chronic (21 days) isolation, crowding, swimming isolation plus combined (chronic stress). Changes plasma levels corticosterone were studied by radioimmunoassay (RIA). results obtained Western immunoblotting showed that both stressors led a significant decrease...

The study deals with manganese superoxide dismutase, copper, zinc and catalase activities in brain cortex of Wistar rats exposed to acute stress (immobilization or cold for 2 h), chronic (long-term isolation long-term forced swimming 21 days), combined chronic/acute stress. We observed that i) single episodes by immobilization increased activity both dismutases; ii) types stresses significantly elevated all examined enzymes; iii) social was a much stronger stressor than physical swimming;...

We previously found that compared to patients with benign uterine diseases (polyps, myomas), premalignant (hyperplasia simplex and complex) malignant (adenocarcinoma) lesions had enhanced lipid peroxidation altered antioxidant enzyme (AOE) activities. To further elucidate the mechanism of observed changes, we examined protein mRNA levels copper-zinc superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPx), reductase (GR), transcription factor Nrf2. also correlations AOE...

(1) Background: Physical activity may cause an imbalance in the major functions of human body. This study aimed to investigate effects endurance running training on parameters antioxidant defense system (SOD, CAT, GPx, GR, GSH), LPO (malondialdehyde, MDA), and stress hormones (A, NA) young healthy, previously untrained men. (2) Methods: The program was as follows: 8 weeks running, three times per week; duration a single session 30-70 min, intensity twice week so-called extensive zone, once...

The oxidative stress contributes to all three phases of carcinogenesis and represents a concomitant condition in renal cell carcinoma (RCC). RCC is the most common type neoplasm kidney, despite numerous studies set predictive prognostic markers survival are still unknown. aim our study was examine relation between antioxidant (AO) status overall (OS) patients.Our included 95 patients with RCC, who underwent radical nephrectomy. We analysed role enzymes (superoxide dismutase, catalase,...