A5070129105- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Photochemistry and Electron Transfer Studies
- Advanced Chemical Physics Studies
- Spectroscopy and Quantum Chemical Studies
- Carbohydrate Chemistry and Synthesis
- HIV Research and Treatment
- Galectins and Cancer Biology
- Mass Spectrometry Techniques and Applications
- Nonlinear Optical Materials Research
- HER2/EGFR in Cancer Research
- Cytomegalovirus and herpesvirus research
- Toxin Mechanisms and Immunotoxins
- Respiratory viral infections research
- Nanoparticle-Based Drug Delivery
- CAR-T cell therapy research
- Escherichia coli research studies
- Transgenic Plants and Applications
- Chemical Synthesis and Analysis
- Chemical Reactions and Mechanisms
- Molecular Spectroscopy and Structure
- HIV/AIDS drug development and treatment
- Influenza Virus Research Studies
Scripps Research Institute
2021-2025
Institute of Atomic and Molecular Sciences, Academia Sinica
2012-2019
Genomics Research Center, Academia Sinica
2016-2018
National Tsing Hua University
2012-2015
Academia Sinica
2012-2015
Institute of Molecular Biology, Academia Sinica
2013-2014
Significance The carbohydrate components of glycoproteins are known to affect the structure and function glycoproteins, thus it is important develop effective tools manipulate optimize glycan with therapeutic significance. This study demonstrates discovery endoglycosidases for remodeling glycans on Herceptin, a monoclonal antibody used in treatment breast cancer, its effector functions, especially antibody-dependent cellular cytotoxicity (ADCC). In addition, method has been developed...
Significance Influenza A virus (IAV) is a major threat to global public health, and so understanding the biology of IAV essential develop antiflu vaccines therapeutics. Here, we show links between viral surface glycosylation function. The HA modulates infectivity, host immune response; NA affects its structure, activity, specificity, thermostability regulate release virulence. In addition, using live attenuated without stalk catalytic domains as vaccine can strongly induce IAV-specific CD8 +...
Glycosylation of humanized antibody at Fc-Asn297 significantly affects the Fc-mediated killing target cells through effector functions, especially antibody-dependent cellular cytotoxicity (ADCC), cell-mediated phagocytosis (ADCP), and vaccinal effect (ADVE). Previous studies showed that therapeutic immunoglobulin G (IgG) antibodies with α2,6-sialyl complex type (SCT) glycan attached to exhibited optimal binding Fc receptors on associated ADCC, ADCP, ADVE. However, production homogeneous...
The development of an HIV vaccine has been hampered by the extraordinary mutability and genetic diversity virus, particularly substantial sequence gp120 gp 41 envelope glycoproteins existing in more than 2000 variants. highly diverse glycans on spikes are commonly considered as immunologically silent self-antigens; however, discovery potent broadly neutralizing antibodies (bNAbs) from patients targeting viral surface raised a major question about origin their antigens. Recent epitope mapping...
A comprehensive structure–activity relationship study on antibody Fc-glycosylation has been performed using the chimeric anti-SSEA4 chMC813-70 as a model.
We report the vibronic and cation spectra of four rotamers m-methoxystyrene, recorded by using two-color resonant two-photon ionization mass-analyzed threshold techniques. The excitation energies S1← S0 electronic transition are found to be 32 767, 907, 33 222, 281 cm−1, corresponding adiabatic 65 391, 64 977, 114, 525 cm−1 for these isomeric species. Most observed active vibrations in electronically excited S1 cationic ground D0 states involve in-plane ring deformation substituent-sensitive...
Abstract Antibody drug conjugates (ADC) are an emerging class of pharmaceuticals consisting cytotoxic agents covalently attached to antibody designed target a specific cancer cell surface molecule followed by internalization and intracellular release payload exhibit its anticancer activity. Targeted delivery variety cells has been demonstrated have significant enhancement in clinical efficacy dramatic reduction off‐target toxicity. Site‐specific conjugation the is highly desirable for...
We report the vibrational spectra of o-fluorophenylacetylene (OFPA), m-fluorophenylacetylene (MFPA), and p-fluorophenylacetylene (PFPA) in electronically excited S1 cationic ground D0 states. These new data show that relative location fluorine atom with respect to acetylenic group can influence transition energy molecular vibration. The adiabatic ionization energies these structural isomers follow order: PFPA < OFPA MFPA. It is found geometries molecules state resemble those state. Detailed...
We applied the two-color resonant two-photon ionization and mass-analyzed threshold techniques to record vibronic cation spectra of 2,4-difluorophenol. As supported by our theoretical calculations, only cis form 2,4-difluorophenol involves in photoexcitation pulsed field processes. The band origin <TEX>$S_1{\leftarrow}S_0$</TEX> electronic transition cis-2,4-difluorophenol appears at 35 647 <TEX>${\pm}2cm^{-1}$</TEX> adiabatic energy is determined be 70 <TEX>$030{\pm}5cm^{-1}$</TEX>,...
ABSTRACT Glycosylation of antibody plays an important role in Fc-mediated killing tumor cells and virus-infected through effector functions such as antibody-dependent cellular cytotoxicity (ADCC), dependent cell-mediated phagocytosis (ADCP) vaccinal effect. Previous studies showed that therapeutical humanized antibodies with α2-6 sialyl complex type (SCT) glycan attached to Fc-Asn297 exhibited optimal binding the Fc receptors on associated ADCC, ADCP However, production homogeneous Fc-SCT...
Vibrationally resolved cation spectra of 1-methylnaphthalene (1MN) and 2-methylnaphthalene (2MN) were obtained using the two-color resonant two-photon mass-analyzed threshold ionization (MATI) spectroscopy. MATI through via several intermediate vibronic levels. Due to hindrance effect, no spectral features related methyl torsion observed in 1MN. By contrast, most in-plane ring deformation vibrations 2MN found couple with because its small internal rotational barrier. These experimental...
We applied the two-colour resonant two-photon ionisation and mass-analysed threshold techniques to record vibrationally resolved spectra of selected rotamers 35Cl 37Cl isotopologues 4-chloro-3-fluorophenol in electronically excited S1 cationic ground D0 states. The band origins ← S0 electronic transition adiabatic energies cis trans are determined be 35,233 ± 2 35,405 cm−1, 69,334 5 69,460 respectively. general spectral features two nearly identical. Most observed active vibrations result...
While the improved treatment of human immunodeficiency virus type 1 (HIV-1) infection is available, development an effective and safe prophylactic vaccine against HIV-1 still unrealized goal. Encouragingly, discovery broadly neutralizing antibodies (bNAbs) from positive patients that are capable a broad spectrum isolates various clades has accelerated progress in past few years. Some these bNAbs recognize N-glycans on viral surface gp120 glycoprotein. We have been interested using glycan...
We present a highly efficient way for the rapid preparation of wide range N-linked oligosaccharides (estimated to exceed 20,000 structures) that are commonly found on human glycoproteins. To achieve desired structural diversity, strategy began with chemo-enzymatic synthesis three kinds oligosaccharyl fluoride modules, followed by their stepwise α-selective glycosylations at 3-O and 6-O positions mannose residue common core trisaccharide having crucial β-mannoside linkage. further attached...