A5046751535- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- T-cell and Retrovirus Studies
- Herpesvirus Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Toxoplasma gondii Research Studies
- Virus-based gene therapy research
- Microbial infections and disease research
- Parasitic Infections and Diagnostics
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Poxvirus research and outbreaks
- Viral Infections and Vectors
- Agriculture and Farm Safety
- Plant Virus Research Studies
- Animal health and immunology
- Viral Infectious Diseases and Gene Expression in Insects
- Cytomegalovirus and herpesvirus research
- Virology and Viral Diseases
- Burkholderia infections and melioidosis
- Invertebrate Immune Response Mechanisms
- Sex work and related issues
- Effects of Environmental Stressors on Livestock
- Parvovirus B19 Infection Studies
- Peanut Plant Research Studies
Centro Panamericano de Fiebre Aftosa
2020-2024
Pan American Health Organization (Brasil)
2020-2023
Instituto Biológico
2012-2022
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2020
Instituto Pasteur
2015
Universidade Federal Fluminense
2014
Ministry of Agriculture, Livestock, and Food Supply
2014
Secretaria de Agricultura e Abastecimento
2003-2013
Universidade Federal de Campina Grande
2012
Universidade de São Paulo
2008
Abstract We describe a vaccinialike virus, Araçatuba associated with cowpoxlike outbreak in dairy herd and related case of human infection. Diagnosis was based on virus growth characteristics, electron microscopy, molecular biology techniques. Molecular characterization the done by using polymerase chain reaction amplification, cloning, DNA sequencing conserved orthopoxvirus genes such as vaccinia factor (VGF), thymidine kinase (TK), hemagglutinin. used VGF-homologous TK gene nucleotide...
O vírus da Diarréia Viral Bovina (BVDV) possui distribuição mundial e é considerado um dos principais patógenos de bovinos. A infecção as enfermidades associadas ao BVDV têm sido descritas no Brasil desde os anos 60. Diversos relatos sorológicos, clínico-patológicos isolamento do agente demonstram a ampla disseminação rebanho bovino brasileiro. Além sorologia positiva em níveis variáveis bovinos corte leite, anticorpos contra o ocasionalmente detectados suínos, javalis, caprinos, cervos...
Vaccinia virus (VACV), the etiological agent of an exanthematic disease, has been associated with several bovine outbreaks in Brazil since end global vaccination campaign against smallpox. It was previously believed that vaccine used for WHO had adapted to unknown wild reservoir and sporadically re-emerging cattle milkers. At present, it is known Brazilian VACV phylogenetically different from vaccinia vaccinal strain, but its origin remains unknown. This study assessed seroprevalence...
Seneca Valley virus (SVV) is a non-enveloped RNA and the only member of Senecavirus A (SVA) species, in genus, Picornaviridae family. SVV infection causes vesicular lesions oral cavity, snout hooves pigs. This clinically indistinguishable from trade-restrictions-related diseases such as foot-and-mouth disease. Other clinical manifestations include diarrhoea, anorexia, lethargy, neurological signs mortality piglets during their first week age. Before this study, Chile was considered free...
O perfil antigênico de 45 herpesvírus (44 bovinos, sendo seis amostras referência BHV-1 e 15 prováveis BHV-1; três BHV-5 20 BHV-5) uma amostra bubalino (BuHV) foi examinado com um painel anticorpos monoclonais (Acms) produzidos contra antígenos bovinos. Para os exames, utilizada a prova imunoperoxidase (IPX) sobre cultivos células infectadas, tendo Acms como primários. A determinação dos padrões reatividade das vírus frente aos permitiu diferenciação entre tipos 1 5. Todas as isoladas casos...
Bovine papillomavirus (BPV) DNA sequences were detected in different tissues, addition to epithelium.Cytogenetic abnormalities observed blood lymphocytes.The presence of more than one virus a single tissue is difficult aspect evaluate, especially when the are tissues that not specifically targeted by virus.BPV and bovine leukemia (BLV) clastogenic, causing chromosome aberrations peripheral lymphocytes.In present study, we investigated simultaneous both viruses
A new outbreak of Vaccinia virus was observed in Southwest region São Paulo State, Brazil. The disease four small dairy farms with manual milking. Lesions were detected cattle and humans previously vaccinated not against smallpox. Although several reports outbreaks have been occurring Brazil, it yet reported this region. This reinforces the viral circulation our country. persons smallpox absence immunity, risk to human health, need for more epidemiologic immunologic studies.
RESUMO Este trabalho teve como objetivo estabelecer a ocorrência de anticorpos contra neosporose, brucelose e leptospirose em alguns rebanhos bubalinos da Amazônia. Foram colhidas 212 amostras sangue búfalas, na Ilha do Marajó (n = 135) região nordeste Estado Pará 77). Examinaram-se submetidas à prova ELISA para pesquisa anti-Neospora caninum utilizando o kit (HerdeChek IDEXX Laboratories, USA); 160 soro fixação complemento (FC) anti-Brucella sp.; 205 através método soroaglutinação...
In late January 2013, 10 nonpregnant Lacaune dairy ewes raised under extensive husbandry management on a farm in Rio de Janeiro, Brazil, presented with the general clinical signs of lethargy, hyporexia, edema face, hyperemia exposed parts skin, mouth lesions, pyrexia, and lameness. Additionally, 2 pregnant died suddenly after onset respiratory signs. The complete blood counts biochemistry analyses showed neutrophilic leukocytosis monocytosis reactive lymphocytes, normocytic normochromic...
Neospora caninum, a cause of abortion and stillbirth in cattle, was studied by histology, immunohistochemistry, nested-PCR, using primers from the Nc5 region genomic DNA (PCR PLUS) ITS1 ribosomal JB).A total 105 fetal samples sent to Centro de Pesquisa e Desenvolvimento Sanidade Animal do Instituto Biológico January 2006 May 2008 were examined for evidence N. caninum.Histological examination revealed 71.4% with non-suppurative inflammation heart, lung, liver, kidney, placenta,...
ABSTRACT: The present study performed a genetic identification of pestiviruses contaminating batches fetal bovine serum (FBS) produced in Brazil from 2006 to 2014. Seventy-three FBS lots were screened by RT-PCR targeting the 5’untranslated region (UTR) pestivirus genome. Thirty-nine (53.4%) positive for RNA and one contained infectious virus. Nucleotide sequencing phylogenetic analysis 5’UTR revealed 34 (46.6%) containing viral diarrhea virus type 1 (BVDV-1), being 23 BVDV-1a (5’ UTR...
For supporting the Brazilian bovine encephalitis surveillance program this study examined differential diagnosis of Neospora caninum in central nervous system (CNS) by histological analysis (HE staining), immunohistochemistry (IHC), and nested-PCR using a set primers from Nc5 region genomic DNA ITS1 ribosomal DNA. A sample 302 cattle presenting neurological syndrome negative for rabies, aged 0 to 18 years, herds 10 states was evaluated N. January 2007 April 2010. All specimens tested with...
Abstract Bluetongue (BT), caused by virus (BTV), is a disease that affects ruminants such as cattle, sheep, goats and deer. BTV transmitted female midges of the genus Culicoides . In Brazil, information on prevalence in cattle limited, so objective this work was to identify serotypes cattle. The State São Paulo divided into seven cattle‐producing regions, each them, 300 farms were randomly selected. One animal from farm (out total 1,598 farms) selected its sera tested neutralization...
Bovine leukemia virus (BLV) was investigated in the central nervous system (CNS) of cattle with neurological syndrome. A total 269 CNS samples were submitted to nested-PCR (BLV env gene gp51), and viral genotypes identified. The positive 4.8% (13/269) samples, 2.7% (2/74) presenting at histological examination lesions nonpurulent meningoencephalitis (NPME), whereas 5.6% (11/195) not NPME (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"...
Abstract Bovine coronavirus ( BC oV) is one of the main aetiological agents gastroenteritis in calves, causing significant economic damage to livestock. This study aims characterise oV genetically on basis N gene. A total 114 faecal samples from beef and dairy calves with or without clinical symptoms diarrhoea five Brazilian states (São Paulo, Minas Gerais, Santa Catarina, Mato Grosso Bahia) were evaluated between 2008 2015 by technique Semi‐ nested RT ‐ PCR for gene genealogical analysis....
Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) optimized validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity 101 DNA copies/μL found 4.8% (14/290) positive for OvHV-2. quantitative (qPCR) 100 copy/μL 5.9% (17/290) positivity, 47.1% (8/17) presenting histological...
The aim of the present study was to determine seroprevalence infection by bovine viral diarrhea virus (BVDV) on family farms, as well risk factors associated with infection. In total, 319 blood serum samples were collected from cattle, reproductive age (>24 months) in 24 herds 11 cities brejo microregion Pernambuco. subjected seroneutralization test detect anti-BVDV antibodies. order identify BVDV, a questionnaire epidemiologic applied and followed statistical analysis logistic...
Neosporose é uma enfermidade parasitária causada pelo protozoário Neospora caninum reconhecido como importante causa de abortamento bovino e neuropatia canina. Considerando o isolamento N. a sorologia freqüente em bovinos nossa região, os objetivos do presente trabalho foram avaliar ocorrência anticorpos anti-N. cães da Microrregião Serra Botucatu, Estado SãoPaulo, sua associação ao sexo, idade procedência quanto à zona urbana (exclusivamente cidade), rural (somente chácaras sítios)...
Bovine Viral Diarrhea Virus (BVDV) and Herpesvirus type 1 (BoHV-1) cause reproductive problems in cattle restrictions on international trade animals worldwide. Both infections were detected herds the Paraíba state, Northeastern Brazil, however, spatial distribution geographic identification of positive for these viruses has never been examined. Therefore, aim this study was to describe pattern apparent prevalence estimate identify clustering BVDV BoHV-1 from state Paraíba, Brazil. The...
Glanders is an equine zoonosis caused by Burkholderia mallei that responsible for considerable economic loss. Complement fixation testing (CFT) using warm or cold incubation are recommended the OIE, but many routinely used detection tests may present misleading results. To increase accuracy of glanders diagnosis and establish appropriate protocol in collaboration with National Equine Health Program, seven horses positive kept isolation Brazil were examined fortnightly CFT, microbiological...