A5044874282- Microtubule and mitosis dynamics
- Neuroblastoma Research and Treatments
- Cancer-related Molecular Pathways
- Genomic variations and chromosomal abnormalities
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Gastric Cancer Management and Outcomes
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- Cancer Genomics and Diagnostics
- Hippo pathway signaling and YAP/TAZ
- Colorectal and Anal Carcinomas
- Colorectal Cancer Surgical Treatments
- Chromosomal and Genetic Variations
- Telomeres, Telomerase, and Senescence
- Renal and related cancers
- Sarcoma Diagnosis and Treatment
- Cancer therapeutics and mechanisms
- Nuclear Structure and Function
- Lung Cancer Treatments and Mutations
- Epigenetics and DNA Methylation
- Signaling Pathways in Disease
- Ubiquitin and proteasome pathways
- Wnt/β-catenin signaling in development and cancer
Tokai University Hachioji Hospital
2019-2024
Saga-Ken Medical Centre Koseikan
2018-2023
Life Science Institute
2018-2020
Hiroshima University
2005-2015
Saitama Cancer Center
2012-2014
Advanced Cancer Therapeutics
2014
Japan Organization of Occupational Health and Safety
2009
Osaka Rosai Hospital
2009
University of Cincinnati Medical Center
2004-2007
Yamaguchi University
2001-2004
Abstract Cancer‐prone syndrome of premature chromatid separation (PCS syndrome) with mosaic variegated aneuploidy (MVA) is a rare autosomal recessive disorder characterized by growth retardation, microcephaly, childhood cancer, all chromosomes, and mosaicism for various trisomies monosomies. Biallelic BUB1B mutations were recently reported in five eight families MVA (probably identical to the PCS syndrome). We here describe molecular analysis (encoding BubR1) seven Japanese syndrome....
The primary cilium is an antenna-like, microtubule-based organelle on the surface of most vertebrate cells for receiving extracellular information. Although cilia form in quiescent phase, ciliary disassembly occurs when re-enter proliferative phase. It was shown that a mitotic kinase, Polo-like kinase 1 (PLK1), required cell-proliferation-coupled disassembly. Here, we report kinesin superfamily protein 2A (KIF2A), phosphorylated at T554 by PLK1, exhibits microtubule-depolymerizing activity...
Abstract Centrosome amplification frequently occurs in human cancers and is a major cause of chromosome instability (CIN). In mouse cells, centrosome can be readily induced by loss or mutational inactivation p53. however, silencing endogenous p53 alone does not induce CIN, although high degrees correlation between mutation CIN/centrosome cancer detected, suggesting the presence additional regulatory mechanism(s) cells that ensures numeral integrity centrosomes genomic integrity. Cyclin E,...
<i>Background:</i> Cutaneous squamous-cell carcinoma (SCC) sometimes causes lymph node metastasis and results in poor prognosis. However, little is known about cytogenetic alterations underlying tumor progression or metastasis. The aim of the present study was to investigate genetic aberrations expression epidermal growth factor receptor (EGFR) metastatic SCC skin. <i>Methods:</i> We undertook comparative genomic hybridization (CGH) analysis 4 specimens which were...
Asymmetric cell division (ACD) is believed to be a physiological event that occurs during development and tissue homeostasis in large variety of organisms. ACD produces two unequal daughter cells, one which resembles multipotent stem and/or progenitor cell, whereas the other has potential for differentiation. Although recent studies have shown balance between self-renewal differentiation potentials precisely controlled alterations may lead tumorigenesis Drosophila neuroblasts, it largely...
Abstract Asymmetric cell division (ACD) is a physiologic process during development and tissue homeostasis. ACD produces two unequal daughter cells: one has stem/progenitor activity the other potential for differentiation. Recent studies showed that misregulation of balance between self-renewal differentiation by may lead to tumorigenesis in Drosophila neuroblasts. However, it still largely unknown whether human cancer stem–like cells exhibit or not. Here, using neuroblastoma as an model, we...
Mortalin is a member of Hsp70 chaperoning protein family involved in various cellular functions. Through the search kinases that mortalin physically interact with, we identified Mps1 as such kinase. kinase has been implicated regulation centrosome duplication and mitotic checkpoint response. binds to Mps1, phosphorylated by on Thr62 Ser65. The then super‐activates feedback manner. previously shown localize centrosomes, be duplication. We found centrosomal localization depends presence Mps1....
A human placenta cDNA expression library was screened for genes inducing flat reversion when transfected into a v‐K‐ras ‐transformed NIH3T3 cell line, DT. One such gene found to encode Kunitz‐type serine protease inhibitor, tissue factor pathway inhibitor‐2 (TFPI‐2). While the TFPI‐2 mRNA can be detected in normal fibroblasts (MRC‐5), it is down‐regulated MRC‐5 cells expressing an activated H‐ras oncogene and fibrosarcoma HT1080. Restored of HT1080 resulted suppression matrix invasion...
Glioblastoma is characterised by invasive growth and a high degree of radioresistance. Survivin, regulator chromosome segregation, highly expressed known to induce radioresistance in human gliomas. In this study, we examined the effect survivin suppression on radiosensitivity malignant glioma cells, while focusing centrosome aberration instability (CIN). We suppressed small interfering RNA transfection, using clonogenic assay trypan blue exclusion U251MG (p53 mutant) D54MG wild type) cells....
Abstract CD133 is a transmembranous protein that mainly localises to the plasma membrane in haematopoietic and neural stem cells as well cancer cells. Although also cytoplasm, mechanism of action function cytoplasmic currently remain unknown. We herein demonstrated when Src family kinase activity weak, interacts with HDAC6 transported pericentrosomal region after internalization endosome formation via dynein-based traffic system. Pericentrosomal then recycled recycling endosomes. At region,...
Glioblastoma (GBM) is a malignant tumor with high recurrence rate and has very poor prognosis in humans. The median survival still <2 years. Therefore, new treatment strategy should be established. Recently, this cancer been thought to heterogeneous, consisting of stem cells (CSCs) that are self-renewable, multipotent, resistant. So various strategies targeting glioma stem-like (GSCs) have investigated. This study focused on GSCs through the induction differentiation using bone morphogenetic...
Abstract BUB1B and RASSF1A genes play specific roles in the mitotic checkpoint, their defects may cause chromosome instability or aneuploidy mouse fibroblasts human cancer cell lines; however, few studies have reported a correlation between these changes tumor samples. We examined abnormalities 25 Wilms tumors by metaphase comparative genomic hybridization, classified them into 14 hyperdiploid (50 ≥ chromosomes), 2 near‐or‐pseudodiploid, 9 diploid tumors. also various molecular aspects of ,...
CD133, also called prominin-1, is widely known as a cancer stem cell marker, and its high expression correlates with poor prognosis in many cancers. CD133 was originally discovered plasma membranous protein stem/progenitor cells. It now that Src family kinases phosphorylate the C-terminal of CD133. However, when kinase activity low, not phosphorylated by preferentially downregulated into cells through endocytosis. Endosomal then associates HDAC6, thereby recruiting it to centrosome via...
Little is known about the genetic changes associated with DNA ploidy in gastric cancer (GC). The aim of this study was to identify recurrent or specific chromosomal regions sequence copy number aberrations (DSCNAs) that might harbor genes aneuploidy GC.We analyzed DSCNAs comparative genomic hybridization and by laser scanning cytometry 16 primary intestinal-type GCs.All GCs examined showed at least one DSCNA (loss gain); eight were diploid (DD) tumors aneuploid (DA) tumors. frequent (>30%)...
Malignant tumors have telomerase activity, which is thought to play a critical role in tumor growth. However, the relation between activity and genomic DNA status cells poorly understood. In present study, we examined 13 clear cell type renal carcinomas (CRCCs) with similar clinicopathologic features by telomeric repeat amplification protocol assay (TRAP). Based on TRAP results, divided CRCCs into two groups: high group low/no group. We then analyzed aberration, ploidy, telomere these groups...