A5021617420- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Cancer Research and Treatments
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Pancreatic and Hepatic Oncology Research
- Antimicrobial Peptides and Activities
- Chemical Synthesis and Analysis
- Carbon Nanotubes in Composites
- Graphene and Nanomaterials Applications
- Glioma Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Nanoparticles: synthesis and applications
- HER2/EGFR in Cancer Research
- Immune Response and Inflammation
- Antifungal resistance and susceptibility
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Advanced biosensing and bioanalysis techniques
University of Lausanne
2009-2023
FHNW University of Applied Sciences and Arts
2020
University of Basel
2015-2019
University Hospital of Basel
2015-2019
Vaximm (Germany)
2017-2018
Centre National de la Recherche Scientifique
2003-2009
Immunologie, Immunopathologie et Chimie Thérapeutique
2005-2009
Institut de Biologie Moléculaire et Cellulaire
2005-2009
University of Trieste
2007
Université de Montpellier
2003
In chronic viral infections, CD8+ T cells become functionally deficient and display multiple molecular alterations. contrast, only little is known of self- tumor-specific from mice humans. Here we determined profiles melanoma patients. peripheral blood patients vaccinated with CpG the antigen Melan-A/MART-1 peptide, found functional effector cell populations, small but nevertheless significant differences in specific for persistent herpesviruses (EBV CMV). Melan-A/MART-1–specific isolated...
Tasty tubes: Multiwalled carbon nanotubes functionalized with fluorescein isothiocyanate and amphotericin B (AmB) have been shown to be rapidly internalized by mammalian cells (see picture) without the toxic effects typically displayed upon treatment AmB. Furthermore, modified drug exhibits a higher antifungal activity than native
Multi-walled carbon nanotubes have been covalently functionalized via 1,3-dipolar cycloaddition of azomethine ylides with orthogonally protected amino functions that can be selectively deprotected and subsequently modified drugs fluorescent probes.
VXM01 is a first-in-kind orally applied tumor vaccine based on live attenuated Salmonella typhi carrying an expression plasmid encoding VEGFR2, antigen expressed vasculature and stable accessible target for anti-angiogenic intervention. A recent randomized, placebo-controlled, phase I dose-escalation trial in advanced pancreatic cancer patients demonstrated safety, immunogenicity transient, T-cell response-related activity of four priming vaccinations within one week. We here evaluated...
Highlights•Microtubule destabilization in dendritic cells drives DC maturation and T cell activation•GEF-H1 is released from microtubules, leading to its release triggers the RhoA-JNK-c-Jun signaling axis AP-1 transcriptional response•GEF-H1 critical for maturation, antigen cross-presentation, anti-tumor immunitySummaryDendritic (DC) activation a step responses. Certain chemotherapeutics can influence function. Here we demonstrate that chemotherapy capable of microtubule has direct effects...
Begehrte Röhren: Mehrwandige Kohlenstoffnanoröhren, die mit Fluoresceinisothiocyanat und Amphotericin B (AmB) funktionalisiert wurden, werden von Säugerzellen schnell internalisiert (siehe Bild), ohne toxischen Wirkungen zu zeigen, typisch für Behandlungen AmB sind. Zudem wirkt das modifizierte Medikament stärker antimykotisch als natives AmB. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2001/2005/z501613_s.pdf or from author....
Immunotherapy of cancer is often performed with altered "analog" peptide Ags optimized for HLA class I binding, resulting in enhanced immunogenicity, but the induced T cell responses require further evaluation. Recently, we demonstrated fine specificity differences and recognition naturally presented Ag by cells after vaccination natural Melan-A/MART-1 peptide, as compared analog peptide. In this study, TCR primary structures 1489 HLA-A*0201/Melan-A(26-35)-specific CD8 derived from both...
We have previously shown that vaccination of HLA-A2 metastatic melanoma patients with the analogue Melan-A(26-35(A27L)) peptide emulsified in a mineral oil induces ex vivo detectable specific CD8 T cells. These are further enhanced when TLR9 agonist is codelivered same vaccine formulation. Interestingly, can be efficiently recognized by HLA-DQ6-restricted CD4 used HLA-DQ6 multimers to assess T-cell response both healthy individuals and patients. report majority carry high frequencies...
Experimental models demonstrated that therapeutic induction of CD8 T cell responses may offer protection against tumors or infectious diseases providing cells have sufficiently high TCR/CD8:pMHC avidity for efficient Ag recognition and consequently strong immune functions. However, comprehensive characterization in clinically relevant situations has remained elusive. In this study, using the novel NTA-His tag-containing multimer technology, we quantified TCR:pMHC dissociation rates (koff)...
Synthetic multivalent ligands, owing to the presence of multiple copies a recognition motif attached central scaffold, can mediate clustering cell surface receptors and thereby function as effector molecules. This paper dissects relationship between structure synthetic ligands targeting CD40, receptor tumor necrosis factor (TNF-R) superfamily. Triggering CD40 signaling in vivo be used enhance immunity against intracellular pathogens or tumors. A series multimeric molecules has been prepared...
Abstract Host resistance to Trypanosoma cruzi infection depends on a type 1 response characterized by strong production of IL-12 and IFN-γ. Amplifying this through CD40 triggering results in control parasitemia. Two newly synthesized molecules (<3 kDa) mimicking trimeric CD40L (mini CD40Ls-1 -2) bind CD40, activate murine dendritic cells, elicit production. Wild-type but not knockout mice exhibited sharp decrease parasitemia mortality when inoculated with T. mixed miniCD40Ls....
Immune protection from infectious diseases and cancer is mediated by individual T cells of different clonal origin. Their functions are tightly regulated but not yet fully characterized. Understanding the contribution each cell will improve prediction immune based on laboratory assessment T-cell responses. Here we developed techniques for simultaneous molecular functional single CD8 directly ex vivo. We studied two groups patients with melanoma after vaccination closely related tumor...
Phenotypic and functional cell properties are usually analyzed at the level of defined populations but not single cells. Yet, large differences between individual cells may have important consequences. It is likely that T-cell–mediated immunity depends on polyfunctionality T cells, rather than sum functions responding T-cell subpopulations. We performed highly sensitive single-cell gene expression profiling, allowing direct ex vivo characterization virus-specific tumor-specific from healthy...
TCRep 3D is an automated systematic approach for TCR-peptide-MHC class I structure prediction, based on homology and ab initio modeling. It has been considerably generalized from former studies to be applicable large repertoires of TCR. First, the location complementary determining regions target sequences are automatically identified by a sequence alignment strategy against database TCR Vα Vβ chains. A structure-based ensures identification CDR3 loops. The CDR then modeled in environment...
2017 Background: VXM01 consists of an attenuated Salmonella typhi Ty21a carrying a plasmid encoding for vascular endothelial growth factor receptor (VEGFR)-2. The bacterium is vector via the oral route administration into Peyer's plaques. vaccine elicits systemic T-cell response targeting VEGFR-2. This trial examined safety and tolerability, clinical immunogenic to after at least four vaccinations [106 or 107 colony-forming units (CFU)] in patients with progressive glioblastoma who have...
The C3-symmetric molecule 1 has been previously shown to mimic CD40 ligand (CD40L) homotrimers and display effector functions. This consists of a cyclic hexapeptide core containing the repetition D-Ala-L-Lys motif. side chains lysine residues have modified by appending CD40L-derived sequence 143Lys-Gly-Tyr-Tyr146via 6-aminohexanoic acid residue as spacer. present report describes general solid-phase synthesis approach related trimeric architectures. In addition, their binding properties well...
Pharmaceutical manufacturing relies on rigorous methods of quality control drugs and in particular the physico-chemical functional characterizations monoclonal antibodies. To that end, robust bioassays are very often limited to reporter gene assays use immortalized cell lines supposed mimic immune cells such as natural killer (NK) detriment primary materials, which appreciated for their biological validity but also difficult exploit due great diversity between individuals. Here, we...