A5073665392- Adipose Tissue and Metabolism
- Metal complexes synthesis and properties
- Redox biology and oxidative stress
- Exercise and Physiological Responses
- Muscle Physiology and Disorders
- Muscle metabolism and nutrition
- Fungal and yeast genetics research
- Adipokines, Inflammation, and Metabolic Diseases
- Mitochondrial Function and Pathology
- Cardiovascular Function and Risk Factors
- Click Chemistry and Applications
- X-ray Diffraction in Crystallography
- Trace Elements in Health
- Cardiac Fibrosis and Remodeling
- Crystallization and Solubility Studies
- Cancer, Hypoxia, and Metabolism
- Ferrocene Chemistry and Applications
- Organometallic Compounds Synthesis and Characterization
- Protein Tyrosine Phosphatases
- Advanced biosensing and bioanalysis techniques
- Nutrition and Health in Aging
- Computational Drug Discovery Methods
- Cell Adhesion Molecules Research
- Marine Biology and Environmental Chemistry
- N-Heterocyclic Carbenes in Organic and Inorganic Chemistry
University of Florence
2016-2025
Florence (Netherlands)
2015
Don Carlo Gnocchi Foundation
2006
University of Milano-Bicocca
2006
IRCCS Istituto Auxologico Italiano
2006
The solution behavior of auranofin, Et3PAuCl and Et3PAuI, as well their interactions with hen egg white lysozyme, single strand oligonucleotide, ds-DNA were comparatively analyzed through NMR spectroscopy, ESI-MS, ethidium bromide displacement, DNA melting viscometric tests. cytotoxic effects toward representative colorectal cancer cell lines found to be strong similar in the three cases a good correlation could established between cytotoxicity ability inhibit thioredoxin reductase;...
Biological aging, characterized by changes in metabolism and physicochemical properties of cells, has an impact on public health. Environment lifestyle, including factors like diet physical activity, seem to play a key role healthy aging. Several studies have shown that regular activity can enhance antioxidant defense mechanisms, the enzymes such as superoxide dismutase (SOD), catalase, glutathione peroxidase. However, intense or prolonged exercise also lead increase reactive oxygen species...
Background— The degree of pulmonary hypertension in healthy subjects exposed to acute hypobaric hypoxia at high altitude was found be related increased plasma endothelin (ET)-1. aim the present study investigate effects ET-1 antagonism on hypertension, renal water, and sodium balance under prolonged exposure high-altitude–associated hypoxia. Methods Results— In a double-blind fashion, volunteers were randomly assigned receive bosentan (62.5 mg for 1 day 125 following 2 days; n=10) or placebo...
In recent years, a few successful attempts were made to repurpose the clinically approved antiarthritic gold drug, Auranofin (AF), as an anticancer agent. The present study shows that iodido(triethylphosphine)gold(I) complex, (Et3PAuI hereafter)—an AF analogue where thiosugar ligand is simply replaced by one iodide ligand—manifests solution chemistry resembling of and exerts similar cytotoxic proapoptotic effects on A2780 human ovarian cancer cells in vitro. However, when evaluated...
The effects of Auranofin (AF) on protein expression and oxidation in A2780 cancer cells were investigated through a strategy based simultaneous proteomics redox determinations. Bioinformatics analysis the data supports view that most critical cellular changes elicited by AF treatment consist thioredoxin reductase inhibition, alteration cell state, impairment mitochondrial functions, metabolic associated with conversion to glycolytic phenotype, induction ER stress. occurrence above was...
Au(NHC) and Au(NHC)2, i.e. a monocarbene gold(I) complex the corresponding bis(carbene) complex, are two structurally related compounds, endowed with cytotoxic properties against several cancer cell lines. Herein, we explore molecular cellular mechanisms at basis of their cytotoxicity in A2780 human ovarian cells. Through comparative proteomic analysis, demonstrated that number modulated proteins is far larger Au(NHC)2-treated than Au(NHC)-treated Both gold compounds mainly affected...
The biological aging, characterized by changing in metabolism and physicochemical properties of cells, has an impact on public health. Environment lifestyle including factors like diet physical activity seems to play a key role healthy ageing. Several studies have shown that regular can enhance antioxidant defence mechanisms, the enzymes such as superoxide dismutase (SOD), catalase, glutathione peroxidase. However, intense or prolonged exercise also lead increase ROS production temporarily,...
We investigated the effect of growing on lactate instead glucose in human cardiomyocyte assessing their viability, cell cycle activity, oxidative stress and metabolism by a proteomic metabolomic approach. In previous studies performed elite players, we found that adaptation to exercise is characterized chronic high plasma level lactate. Lactate considered not only an energy source but also signalling molecule referred as "lactormone"; heart one major recipients exogenous With this mind, used...
Abstract The silver(I) N ‐heterocyclic carbene (NHC) complex bis(1‐(anthracen‐9‐ylmethyl)‐3‐ethylimidazol‐2‐ylidene) silver chloride ([Ag(EIA) 2 ]Cl), bearing two anthracenyl fluorescent probes, has been synthesized and characterized. [Ag(EIA) ]Cl is stable in organic solvents under physiological conditions, shows potent cytotoxic effects vitro toward human SH‐SY5Y neuroblastoma cells. interactions of with a few model biological targets have studied as well its ability to be internalized...
Aurothiomalate (AuTM) is an FDA-approved antiarthritic gold drug with unique anticancer properties. To enhance its activity, we prepared a bioconjugate human apoferritin (HuHf) by attaching some AuTM moieties to surface protein residues. The reaction of excess yielded single adduct, that was characterized ESI MS and ICP-OES analysis, using three mutant ferritins trypsinization experiments. adduct contains ~3 atoms per ferritin subunit, arranged in small cluster bound Cys90 Cys102. MD...
NMR-based metabolomics is used to characterize the metabolic phenotype of auranofin treatment in A2780 ovarian cancer cells. The most distinctive trait an early and evident increase intracellular GSH, a key molecule cell redox metabolism.
Ferritin may serve as a nanocarrier for the selective delivery of anticancer metallodrugs. Here, we have prepared and characterized well-defined conjugate between gold drug auranofin apoferritin containing ∼14 atoms per nanocage ([email protected] hereafter). Site directed mutagenesis combined with ESI MS ICP-OES experiments revealed that binding occurs exclusively at cysteines 90 102. [email was found to manifest potent cytotoxic properties against A2780 cancer cells overcome cisplatin...