A5044710963- Photonic Crystals and Applications
- Photonic and Optical Devices
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Orbital Angular Momentum in Optics
- Optical Coherence Tomography Applications
- 3D Surveying and Cultural Heritage
- Metamaterials and Metasurfaces Applications
- Advanced Vision and Imaging
- Remote Sensing and LiDAR Applications
- Advanced Antenna and Metasurface Technologies
- Near-Field Optical Microscopy
- Advanced Optical Imaging Technologies
- Single-cell and spatial transcriptomics
- Advanced Electron Microscopy Techniques and Applications
- Plasmonic and Surface Plasmon Research
- Click Chemistry and Applications
- Microwave Engineering and Waveguides
- Photoacoustic and Ultrasonic Imaging
- Image Processing Techniques and Applications
- Diffusion and Search Dynamics
- Surface Roughness and Optical Measurements
- Biotin and Related Studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Ubiquitin and proteasome pathways
Indian Institute of Technology Kanpur
2024
Northwestern University
2019-2021
Indian Institute of Technology Delhi
2012-2016
Darbhanga Medical College and Hospital
2011
Versatile, sterically accessible imaging systems capable of in vivo rapid volumetric functional and structural deep the brain continue to be a limiting factor neuroscience research. Towards overcoming this obstacle, we present integrated one- two-photon scanned oblique plane illumination (SOPi, /sōpī/) microscopy which uses single front-facing microscope objective provide light-sheet scanning based capability at subcellular resolution. Our planar scan-mirror optimized architecture allows for...
Abstract The vertebrate brain consists of diverse neuronal types, classified by distinct anatomy and function, along with divergent transcriptomes proteomes. Defining the cell-type specific neuroproteomes is important for understanding development functional organization neural circuits. This task remains challenging in complex tissue, due to suboptimal protein isolation techniques that often result loss information incomplete capture subcellular compartments. Here, we develop a genetically...
We present a large-area and single-step fabrication approach based on phase spatial light modulator (SLM)-assisted interference lithography for the realization of submicrometer photonic structures photoresist. A multimirror beam steering unit is used to reflect SLM-generated phase-engineered beams leading large angle between interfering while also preserving area plane beams. Both translational rotational periodic are experimentally realized. This increases flexibility fabricate more complex...
This Letter presents the first demonstration of multi-tile stitching for large scale 3D imaging in single objective light-sheet microscopy. We show undistorted spanning complete zebrafish larvae, and over 1 mm3 volumes thick mouse brain sections. use remote galvo scanning creation develop a processing pipeline tiling across different axes. With improved one photon (1p) tilt-invariant scanned oblique plane illumination (SOPi) microscope presented here, we demonstrate cellular resolution at...
Oblique plane microscopy (OPM) is a single objective light-sheet that performs three dimensional (3D) imaging by axial scan of the generated light-sheet. Recently, multiple techniques for lateral scanning in OPM have emerged. However, their suitability geometrically distortion free 3D imaging, which essentially requires constant tilt scan, has not been evaluated. In this work, we use geometrical optics approach and derive analytical relationship amount variance planar mirror based scanned...
We propose a simple and straightforward method to generate spatially variant lattice structures by optical interference lithography method. Using this method, it is possible independently vary the orientation period of two-dimensional lattice. The consists two steps which are: numerical synthesis corresponding phase mask employing integrated gradient calculations experimental implementation synthesized making use only spatial light modulator in an 4f Fourier filtering setup. As working...
We present a new, single-step approach for generating hexagonal lattice wave field with gradient local basis structure. incorporate this by coherently superposing two (or more) fields, which differ in their structures. The of the resultant is highly dependent on relative strengths constituent and desired spatial modulation structure thus obtained controlling fields. experimental realization achieved using phase-only light modulator (SLM) an optical 4f Fourier filter setup where SLM displayed...
Abstract We present an open access scanned oblique plane microscopy platform Crossbill . It combines a new optical configuration, hardware assembly, systematic alignment protocol, and dedicated control software to provide compact, versatile, high resolution single objective light-sheet platform. The demonstrated configuration yields the most affordable sub-micron system date. add galvanometer enabled tilt-invariant lateral scan for multi-plane, multi-Hz volumetric imaging capability. A...
We present a technique to optically induce defect site in helical lattice wave-field where the combined continues maintain its nondiffracting (ND) nature. This is done by coherently superposing and Bessel beam method of phase engineering. The results are confirmed numerical simulations experimentally as well generating ND displaying numerically calculated pattern on phase-only spatial light modulator. wavelength independent, completely scalable, can easily be used generate or transfer these...
We present a method to combine two periodic lattice wave-fields generate complex dual wave-field which could be employed for microfabrication of corresponding 2D structures. Since the addition is coherent in nature resultant structure highly dependent on relative phase difference between constituent wave-fields. show that it possible have control over pattern by precisely controlling this difference. This enabled making use digitally addressable only spatial light modulator (SLM). provide...
A periodic lattice wavefield with multiple nondiffracting negative defect sites at the desired locations is very useful in photonic crystal device fabrication and other applications. Such a was realized by coherently superposing wavefields Bessel each of sites. The experimental verification done making use phase only spatial light modulator (SLM) basic 4f Fourier filtering geometry where image to be displayed on SLM calculated engineering. Photonic crystals small periods are possible...
In this communication, several coplanar wave guide topologies to the hemispherical DR.A are presented. It is seen that inductive and circular-loop CPW feed configurations offer flexibility excite fundamental TE111 resonant mode of DRA close source free frequency, due presence metallization in coupling region. Dual-band behavior observed with capacitive slot modes, which displaced respect unperturbed modes uninterrupted slot. The radiation characteristics antenna arc also explained.
We present a method to embed multiple non-diffracting defects in 2D square lattice wave-field by employing binary phase mask. Experimental verification using simple 4f-Fourier filtering based is also presented.
Light-sheet microscopy has evolved as an indispensable tool in imaging biological samples. It can image 3D samples at fast speed, with high-resolution optical sectioning, and reduced photobleaching effects. These properties make light-sheet ideal for fluorophores a variety of organisms, e.g. zebrafish, drosophila, cleared mouse brains, etc. While most commercial turnkey systems are expensive, the existing lower cost implementations, OpenSPIM, focused on achieving small or organisms like...
We present an open platform to design, build and operate single objective based oblique light-sheet microscope. Using this platform, here we design a new compact configuration of the