A5072170969- Bacterial Infections and Vaccines
- Mesenchymal stem cell research
- Pneumonia and Respiratory Infections
- Influenza Virus Research Studies
- Immunotherapy and Immune Responses
- Neonatal Respiratory Health Research
- Immune Response and Inflammation
- Asthma and respiratory diseases
- Tissue Engineering and Regenerative Medicine
- Immune Cell Function and Interaction
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Immune cells in cancer
- Parvovirus B19 Infection Studies
- Dermatological and COVID-19 studies
- Chemokine receptors and signaling
- Peripheral Neuropathies and Disorders
- Hematopoietic Stem Cell Transplantation
- Monoclonal and Polyclonal Antibodies Research
- Breastfeeding Practices and Influences
- Respiratory and Cough-Related Research
- Electrospun Nanofibers in Biomedical Applications
- Infant Nutrition and Health
- CAR-T cell therapy research
- Cancer Cells and Metastasis
National University of Ireland, Maynooth
2011-2022
University of Central Lancashire
2019
Food for Health Ireland
2017
National Institute for Biological Standards and Control
1987-2015
Alfred University
2013
National University of Ireland
2004-2012
University College Dublin
1997-2005
Institute of Immunology
2005
Abstract Adult bone marrow derived mesenchymal stem cells offer the potential to open a new frontier in medicine. Regenerative medicine aims replace effete broad range of conditions associated with damaged cartilage, bone, muscle, tendon and ligament. However normal process immune rejection mismatched allogeneic tissue would appear prevent realisation such ambitions. In fact avoid humans animal models. These finding are supported by vitro co-culture studies. Three mechanisms contribute this...
Summary Adult human mesenchymal stromal or stem cells (MSC) can differentiate into a variety of cell types and are candidate cellular therapeutics in regenerative medicine. Surprisingly, these also display multiple potent immunomodulatory capabilities, including allosuppression, making allogeneic therapy possibility. The exact mechanisms involved regulatory T induction by MSC was examined, using purified CD4+ populations well-characterized bone marrow-derived adult MSC. Allogeneic were shown...
Summary The ability of mesenchymal stem cells (MSC) to suppress alloresponsiveness is poorly understood. Herein, an allogeneic mixed lymphocyte response was used as a model investigate the mechanisms MSC-mediated immunomodulation. Human MSC are demonstrated express immunosuppressive cytokines hepatocyte growth factor (HGF), interleukin (IL)-10 and transforming (TGF)-β1 at concentrations that alloresponses in vitro. also cyclooxygenase 1 2 produce prostaglandin E2 constitutively. Blocking...
ABSTRACT The results of phase 3 efficacy trials have shown that acellular and whole-cell pertussis vaccines can confer protection against whooping cough. However, despite the advances in vaccine development, clinical not provided significant new information on mechanism protective immunity Bordetella . Classical approaches based measurement antibody responses to individual antigens failed define an immunological correlate protection. A reliable animal model, predictive potency children,...
Abstract Mesenchymal stem cells (MSCs) inhibit T‐cell activation and proliferation but their effects on individual T‐cell‐effector pathways memory versus naïve T remain unclear. MSC influence the differentiation of CD4 + toward Th17 phenotype was examined. exposed to Th17‐skewing conditions exhibited reduced CD25 IL‐17A expression following co‐culture. Inhibition production persisted upon re‐stimulation in absence MSCs. These were attenuated when cell–cell contact prevented. cultures from...
To cite this article: Kavanagh H, Mahon BP. Allogeneic mesenchymal stem cells prevent allergic airway inflammation by inducing murine regulatory T cells. Allergy 2011; 66 : 523–531. Abstract Background: Adult bone marrow‐derived (MSC) possess potent immune modulatory effects which support their possible use as a therapy for immune‐mediated disease. MSC induce (T reg ) in vitro although the vivo relevance of is not clear. Objective: This study addressed hypothesis that adult marrow...
Abstract Introduction Mesenchymal stromal cells (MSC) have well defined immunomodulatory properties including the suppression of lymphocyte proliferation and inhibition dendritic cell (DC) maturation involving both contact soluble factors. These made MSC attractive candidates for cellular therapy. However, mechanism underlying these characteristics remains unclear. This study sought to investigate mechanisms by which induce a regulatory environment. Method Allogeneic bone marrow mesenchymal...
Umbilical cord tissue represents a unique source of cells with potential for cell therapy applications multiple diseases. Human umbilical tissue-derived (hUTC) are developmentally early stage, homogenous population that HLA-ABC dim, HLA-DR negative, and lack expression co-stimulatory molecules in the unactivated state. The molecule on hUTC may account their reduced immunogenicity, facilitating use allogeneic settings. However, such approaches could be confounded by host innate as natural...
Abstract The incidence of idiopathic pulmonary fibrosis is on the rise and existing treatments have failed to halt or reverse disease progression. Mesenchymal stromal cells (MSCs) potent cytoprotective effects, can promote tissue repair, demonstrated efficacy in a range fibrotic lung diseases; however, exact mechanisms action remain be elucidated. Chemical antagonists short hairpin RNA knockdown were used identify by MSCs promoting wound healing, proliferation, inhibiting apoptosis. Using...
Using a murine respiratory challenge model we have previously demonstrated role for Th1 cells in natural immunity against Bordetella pertussis, but could not rule out antibody. Here that B. pertussis infection of mice with targeted disruptions the genes IFN-γ receptor resulted an atypical disseminated disease which was lethal proportion animals, and characterized by pyogranulomatous inflammation postnecrotic scarring livers, mesenteric lymph nodes kidneys. Viable virulent bacteria were...
Using a murine respiratory infection model, we have demonstrated previously that with Bordetella pertussis or immunization whole-cell vaccine induced antigen-specific Th1 cells, which conferred high level of protection against aerosol challenge. In contrast, an acellular vaccine, consisting the B. components detoxified toxin, filamentous hemagglutinin, and pertactin adsorbed to alum, generated Th2 cells was associated delayed bacterial clearance following this study, addition interleukin-12...
Summary Acute graft-versus-host disease (aGVHD) is a life-threatening complication following allogeneic haematopoietic stem cell transplantation (HSCT), occurring in up to 30–50% of patients who receive human leucocyte antigen (HLA)-matched sibling transplants. Current therapies for steroid refractory aGVHD are limited, with the prognosis suboptimal. Mesenchymal or stromal cells (MSC), heterogeneous population present many tissues, display potent immunomodulatory abilities. Autologous and...
Bone-marrow derived mesenchymal stromal cells (MSCs) have potent immunomodulatory and tissue reparative properties, which may be beneficial in the treatment of inflammatory diseases such as COPD. This study examined mechanisms by human MSCs protect against elastase induced emphysema. Using a novel relevant pre-clinical model emphysema efficacy MSC therapy optimal cell dose were investigated. Protective effects lung through histological examination. Further vivo experiments abilities after...
The Fourth Expert Meeting of the Mesenchymal Stem Cells in Solid Organ Transplantation (MiSOT) Consortium took place Barcelona on October 19 and 20, 2012. This meeting focused translation preclinical data into early clinical settings. position paper highlights main topics explored safety efficacy mesenchymal stem cells as a therapeutic agent solid organ transplantation emphasizes issues (proper timing, concomitant immunossupression, source immunogenicity cells, oncogenicity) that have been...
The current understanding of the function CD4+ T helper (Th) cells in immunity to infectious diseases is that Th1 cells, which secrete interleukin (IL)-2 and interferon-gamma, induce cellular immune responses, whereas Th2 IL-4, IL-5, IL-6, IL-10, provide for humoral immunity. We have used a panel poliovirus-specific murine cell clones mice transgenic human poliovirus receptor evaluate role Th subpopulations protective poliovirus. majority clones, as well polyclonal generated from infected or...
In studies of the mechanism immunity to Bordetella pertussis in a murine respiratory infection model, we have previously demonstrated that natural immunization with whole cell vaccine induces potent protective immune response, which is mediated by T‐helper type‐1 (Th1) cells. contrast an acellular generates Th2 cells and associated delayed bacterial clearance following challenge. present study investigated apparent Th1/Th2 dichotomy acquired examined factors affect their induction or...
A fixation and permeabilization procedure using formaldehyde acetone has been developed which allows immunostaining of intracellular haemoglobin for fluorescence activated cell sorter (FACS) analysis erythrocytes. The treatment preserves antigenicity light-scattering properties. Validation the method was given by correlation F number in adults determined FACS with that assessed microscopic examination smears, direct relationship between beta chain synthesis intensity chain/Hb...
Members of the Burkholderia cepacia complex (Bcc) cause chronic opportunistic lung infections in people with cystic fibrosis (CF), resulting a gradual function decline and, ultimately, patient death. The Bcc is 20 species and rarely eradicated once colonized; therefore, vaccination may represent better therapeutic option. We developed new proteomics approach to identify bacterial proteins that are involved attachment bacteria epithelial cells. Fourteen were reproducibly identified by...