A5036302617- Mechanical Circulatory Support Devices
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Transplantation: Methods and Outcomes
- Cardiac Structural Anomalies and Repair
- Congenital Heart Disease Studies
- Mesenchymal stem cell research
- Renal Transplantation Outcomes and Treatments
- Cardiac and Coronary Surgery Techniques
- Cardiac Arrhythmias and Treatments
- Organ Transplantation Techniques and Outcomes
- Cardiac Arrest and Resuscitation
- Angiogenesis and VEGF in Cancer
- Congenital Diaphragmatic Hernia Studies
- Aortic Disease and Treatment Approaches
- Renal and related cancers
- Atrial Fibrillation Management and Outcomes
- Cardiac Fibrosis and Remodeling
- Pluripotent Stem Cells Research
- Viral Infections and Immunology Research
- Esophageal and GI Pathology
- Cardiac pacing and defibrillation studies
- Cardiac, Anesthesia and Surgical Outcomes
- Cardiac tumors and thrombi
- Coronary Interventions and Diagnostics
Cohen Children's Medical Center
2025
Loma Linda University
2018-2023
Loma Linda University Children's Hospital
2017-2022
Loma Linda University Medical Center
2018-2021
University of Southern California
2015
Columbia University
2004-2011
The University of Melbourne
2011
Columbia University Irving Medical Center
2005-2009
Medical University of South Carolina
2009
University of Pennsylvania
2009
Many adult organs contain stem cells, which are pluripotent and involved in organ maintenance repair after injury. In situ, these cells often have a low cycling rate locate specialized regions (niches). To detect such the kidney, we administered pulse of nucleotide bromodeoxyuridine (BrdU) to rat mouse pups and, long (more than 2-month) chase, examined whether kidney contained population low-cycling cells. We found that BrdU-retaining were very sparse except renal papilla, where they...
Control over cell engraftment, survival, and function remains critical for heart repair. We have established a tissue engineering platform the delivery of human mesenchymal progenitor cells (MPCs) by fully biological composite scaffold. Specifically, we developed method complete decellularization myocardium that leaves intact most elements extracellular matrix, as well underlying mechanical properties. A cell–matrix was constructed applying fibrin hydrogel with suspended onto decellularized...
The primary cardiac response to ischemic insult is cardiomyocyte hypertrophy, which initiates a genetic program culminating in apoptotic myocyte loss, progressive collagen replacement, and heart failure, process termed remodeling. Although few cardiomyocytes at the peri-infarct region can proliferate regenerate after injury, no approaches are known effectively induce endogenous enter cell cycle. We recently isolated, human adult bone marrow, endothelial progenitor cells, or angioblasts, that...
Abstract Native myocardium consists of several cell types, which approximately one‐third are myocytes and most the nonmyocytes fibroblasts. By analogy with monolayer culture in fibroblasts were removed to prevent overgrowth, early attempts engineer utilized populations enriched for cardiac (CMs; ∼80–90% total cells). We hypothesized that pre‐treatment synthetic elastomeric scaffolds (CFs) will enhance functional assembly engineered constructs by creating an environment supportive...
Heart disease is the leading cause of death in US. Following an acute myocardial infarction, a fibrous, noncontractile scar develops, and results congestive heart failure more than 500,000 patients US each year. Muscle regeneration induction new vascular growth to treat ischemic disorders can have significant therapeutic implications. Early studies with chronic systolic left ventricular dysfunction (SLVD) using skeletal myoblasts or bone marrow-derived cells report improvement ejection...
Targeted delivery of mesenchymal precursor cells (MPCs) can modify left ventricular (LV) cellular and extracellular remodeling after myocardial infarction (MI). However, whether to what degree LV may be affected by MPC injection post-MI, these effects are concentration-dependent, remain unknown.Allogeneic MPCs were expanded from sheep bone marrow, direct intramyocardial was performed within the borderzone region 1 hour MI induction (coronary ligation) in at following concentrations: 25x10(6)...
It is becoming increasingly apparent that the architecture and mechanical properties of scaffolds, particularly with respect to mimicking features natural tissues, are important for tissue engineering applications. Acrylated poly(glycerol sebacate) (Acr-PGS) a material can be cross-linked upon exposure ultraviolet light, leading networks tunable degradation through simple changes during Acr-PGS synthesis. For example, number acrylate functional groups on macromer dictates concentration...
The kidney papilla contains a population of cells with several characteristics adult stem cells, including the retention proliferation markers during long chase periods (i.e., they are label-retaining [LRCs]). To determine whether papillary LRCs generate new in normal kidney, we examined cell throughout and found that upper is site enhanced cycling. Using genetically modified mice conditionally expressed green fluorescence protein fused to histone 2B, observed proliferated only its part,...
The objectives of this study are to determine risk factors associated with deep sternal wound infections (DSWIs) following cardiac surgery, and describe their impact on long-term survival. Data was obtained from a departmental database. Analysis included 7,978 consecutive patients who underwent surgery between 1997 2003. To identify for DSWI, regression analysis performed. probability scores logistic were used propensity 2 groups. Kaplan-Meier log-rank test Cox proportional hazard models...
Vitamin D3 up-regulated protein 1 (VDUP1) is a key mediator of oxidative stress on various cellular processes via downstream effects apoptosis signaling kinase (ASK1) and p38 mitogen-activated (MAPK). Here, we report that VDUP1 expression significantly increased in rat hearts following acute myocardial ischemia, suggesting it may have important regulatory cardiac physiological during periods stress. Transfection H9C2 cardiomyoblasts with sequence-specific DNA enzyme to down-regulate mRNA...
Many adult organs contain stem cells, which are pluripotent and involved in organ maintenance repair after injury. In situ, these cells often have a low cycling rate locate specialized regions (niches). To detect such the kidney, we administered pulse of nucleotide bromodeoxyuridine (BrdU) to rat mouse pups and, long (more than 2-month) chase, examined whether kidney contained population low-cycling cells. We found that BrdU-retaining were very sparse except renal papilla, where they...