A5007507581- Biochemical and Structural Characterization
- Silk-based biomaterials and applications
- Urinary Tract Infections Management
- Escherichia coli research studies
- Beetle Biology and Toxicology Studies
- Neurobiology and Insect Physiology Research
- Heat shock proteins research
- Plant Gene Expression Analysis
- Urinary Bladder and Prostate Research
- Microtubule and mitosis dynamics
- Alzheimer's disease research and treatments
- 14-3-3 protein interactions
- Phytochemical compounds biological activities
- Signaling Pathways in Disease
- Monoclonal and Polyclonal Antibodies Research
- Pediatric Hepatobiliary Diseases and Treatments
- Genetics, Bioinformatics, and Biomedical Research
- Enzyme Structure and Function
- Drug Transport and Resistance Mechanisms
- Protein Structure and Dynamics
- Nitrogen and Sulfur Effects on Brassica
- Plant biochemistry and biosynthesis
- Bacterial Genetics and Biotechnology
- Phytochemicals and Antioxidant Activities
- Pediatric Urology and Nephrology Studies
Uppsala University
2014-2024
Chalcone synthase (CHS) catalyzes the first committed step in flavonoid biosynthetic pathway. In this study, cDNA (FhCHS1) encoding CHS from Freesia hybrida was successfully isolated and analyzed. Multiple sequence alignments showed that both conserved active site residues signature were found deduced amino acid of FhCHS1. Meanwhile, crystallographic analysis revealed protein structure FhCHS1 is highly similar to alfalfa CHS2, biochemical results indicated it has an enzymatic role naringenin...
Proteus mirabilis, a Gram-negative uropathogen, is major causative agent in catheter-associated urinary tract infections (CAUTI). Mannose-resistant Proteus-like fimbriae (MR/P) are crucially important for P. mirabilis infectivity and required biofilm formation auto-aggregation, as well bladder kidney colonization. Here, the X-ray crystal structure of MR/P tip adhesin, MrpH, reported. The has fold not previously described contains transition metal center with Zn2+ coordinated by three...
The important uropathogen Proteus mirabilis encodes a record number of chaperone/usher-pathway adhesive fimbriae. Such fimbriae, which are used for adhesion to cell surfaces/tissues and biofilm formation, typically virulence factors in bacterial pathogenesis. Here, the structures receptor-binding domains tip-located two-domain adhesins UcaD (1.5 Å resolution) AtfE (1.58 from two P. fimbriae (UCA/NAF ATF) presented. both similar F17G type fimbrial domains, very despite having only limited...
Proteins can misfold into fibrillar or amorphous aggregates and molecular chaperones act as crucial guardians against these undesirable processes. The BRICHOS chaperone domain, found in several otherwise unrelated proproteins that contain amyloidogenic regions, effectively inhibits amyloid formation toxicity but some cases also prevent non-fibrillar, protein aggregation. Here, we elucidate the basis behind multifaceted activities of domain from Bri2 proprotein. High-confidence AlphaFold2...
Spider silk is a biomaterial with exceptional mechanical toughness, and there great interest in developing biomimetic methods to produce engineered spider silk-based materials. However, the mechanisms that regulate conversion of proteins (spidroins) from highly soluble dope into are not completely understood. The N-terminal domain (NT) Euprosthenops australis dragline protein undergoes conformational quaternary-structure changes monomer at pH above 7 homodimer lower values. Conversion dimer...