A5103276286- Mesenchymal stem cell research
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Hippo pathway signaling and YAP/TAZ
- Connective tissue disorders research
- Angiogenesis and VEGF in Cancer
- Liver Disease and Transplantation
- Cancer Cells and Metastasis
- Liver Disease Diagnosis and Treatment
- Tissue Engineering and Regenerative Medicine
- Microfluidic and Bio-sensing Technologies
- Cardiovascular Function and Risk Factors
- Renal Transplantation Outcomes and Treatments
- Viral Infections and Immunology Research
- Transplantation: Methods and Outcomes
Chung-Ang University
2025
The University of Texas at Austin
2018-2021
Baylor University Medical Center
2019
Saccharomyces cerevisiae is a generally recognized as safe (GRAS) workhorse strain widely used in the food industry for cost-effective production of ingredients. However, heme yield yeast significantly lower than bacteria two main reasons: (1) biosynthetic pathway bifurcated into cytosol and mitochondria, (2) yeast's protoporphyrin-dependent (PPD) thermodynamically unfavorable compared with bacteria's coproporphyrin-dependent (CPD) pathway. To overcome these limitations, PPD CPD pathways...
Abstract Mechanical forces are important in the regulation of physiological homeostasis and development disease. The application mechanical to cultured cells is often performed using specialized systems that lack flexibility throughput other biological techniques. In this study, we developed a high platform for applying complex dynamic cells. We validated system its ability accurately apply parallel stretch 96 well plate format 576 simultaneously. Using system, screened optimized conditions...
In acute decompensated heart failure (ADHF), noninvasive markers that predict morbidity and mortality are limited. Liver stiffness measurement (LSM) increases with hepatic fibrosis; however, it may be falsely elevated in patients ADHF the absence of liver disease. We investigated whether LSM predicts cardiac outcomes ADHF.In a prospective study, we examined 52 without disease between 2016 2017. Patients underwent 2D shear wave elastography (SWE) were followed for 12 months to assess left...
Transgenic methods for direct reprogramming of somatic cells to induced pluripotent stem (iPSCs) are effective in cell culture systems but ultimately limit the utility iPSCs due concerns mutagenesis and tumor formation. Recent studies have suggested that some transgenes can be eliminated by using small molecules as an alternative transgenic iPSC generation. We developed a high throughput platform applying complex dynamic mechanical forces cultured cells. Using this system, we screened...
Stem cell therapies have great promise for revolutionizing treatments cardiovascular disease and other disorders but not yet achieved their potential due to poor efficacy heterogeneity in patient response. Here, we used a novel high throughput screening system optimize the conditioning of mesenchymal stem cells using combinatorial set biochemical factors, pharmacological inhibitors biomechanical forces. Our studies revealed that combination specific kinase complex mechanical strain waveform...
Differentiation of mesenchymal stem cells (MSCs) towards vascular cell types would provide improved therapies for cardiovascular disease. However, studies differentiation MSCs into endothelial or muscle have had mixed contradictory results. We developed a high throughput device capable applying mechanical strain to in multiwell format that allow mechanobiological screening cultured cells. Using this device, we screened conditions maximally increased signaling through the Hippo and TGF-β...
Induced pluripotent stem cells (iPSC) have great promise for providing new models of disease and generating novel cell types from patient derived samples. However, studies on iPSC derivation often involve viral genetic manipulation, which causes concerns in terms using the cellular therapeutics. To that end, we propose a methodology to induce phenotypes mouse embryonic fibroblasts (MEFs) through use mechanical conditioning, without gene editing. achieve this goal, developed high-throughput...