A5061567869- Food Quality and Safety Studies
- Phytoestrogen effects and research
- Pharmacological Effects of Natural Compounds
- TiO2 Photocatalysis and Solar Cells
- Phytochemicals and Antioxidant Activities
- Ecology and Conservation Studies
- Pluripotent Stem Cells Research
- Nutrition, Health and Food Behavior
- Ginger and Zingiberaceae research
- RNA Research and Splicing
- Ginseng Biological Effects and Applications
- Natural product bioactivities and synthesis
- Prenatal Screening and Diagnostics
- Genomics, phytochemicals, and oxidative stress
- Advanced Photocatalysis Techniques
- Epigenetics and DNA Methylation
- Endoplasmic Reticulum Stress and Disease
- Hippo pathway signaling and YAP/TAZ
- Renal and related cancers
- Cell Adhesion Molecules Research
- Advanced Glycation End Products research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Streptococcal Infections and Treatments
- Sesame and Sesamin Research
- Vector-borne infectious diseases
University of Pennsylvania
2024
The University of Texas at Austin
2018-2024
Pusan National University
2008-2023
Nune Eye Hospital
2020
Center for Food Safety and Applied Nutrition
2017-2018
United States Food and Drug Administration
2017-2018
National Institute of Ecology
2017
Joslin Diabetes Center
2015
Ministry of Food and Drug Safety
2014-2015
Institute of Crop Science
2014
Trophectoderm (TE) lineage development is pivotal for proper implantation, placentation, and healthy pregnancy. However, only a few TE-specific transcription factors (TFs) have been systematically characterized, hindering our understanding of the process. To elucidate regulatory mechanisms underlying TE development, here we map super-enhancers (SEs) in trophoblast stem cells (TSCs) as model. We find both prominent master TFs (Cdx2, Gata3, Tead4), >150 that had not previously implicated...
Significance Treatment with bone morphogenetic protein 4 (BMP4) in human primed pluripotent stem cells (PSCs) for generating trophoblast lineage has sparked debate that the resulting are closer to amnion rather than trophoblast. This study reports stem-like (TSLCs) can be generated from PSCs by a short-term treatment of BMP4 without marker expression. In addition, we describe TSLCs self-renewing long-term culture and bipotent as they differentiate into functional extravillous trophoblasts...
Abstract The placenta is an essential organ that supports the growth and development of fetus during pregnancy. However, cell type-specific enhancers transcription factors (TFs), mechanisms underlying maintenance differentiation trophoblast stem (TSC) populations in human remain elusive. Here, using TSCs as a model system, we identify 31,362 are enriched with motifs previously reported TSC-pivotal TFs, including TEAD4, GATA2/3 TFAP2C. Subsequently, 580 super-enhancers (SEs) 549 SE-associated...
Abstract During human pregnancy, extravillous trophoblasts play crucial roles in placental invasion into the maternal decidua and spiral artery remodeling. However, regulatory factors their action mechanisms modulating trophoblast specification have been unknown. By analyzing dynamic changes transcriptome enhancer profile during stem cell to differentiation, we define stage-specific regulators, including an early-stage transcription factor, TFAP2C, multiple late-stage factors....
Approximately, 30% of embryonic stem cells (ESCs) die after exiting self-renewal, but regulators this process are not well known. Yap1 is a Hippo pathway transcriptional effector that plays numerous roles in development and cancer. However, its functions ESC differentiation remain poorly characterized. We first reveal ESCs lacking experience massive cell death upon the exit from self-renewal. subsequently show contextually protects differentiating, self-renewing, hyperactivation apoptotic...
Intercellular communication via gap junctions is thought to play an important role in embryonic cell survival and differentiation. Classical studies demonstrated both dye electrical coupling of cells the inner mass mouse embryos, as well development restrictions against between surrounding trophectoderm. Here we demonstrate extensive junctional human stem (ES) cells, pluripotent isolated from preimplantation blastocysts. Human ES maintained vitro expressed RNA for 18 20 known connexins; only...
Persistence of Orientia tsutsugamushi in HumansWe investigated the persistence viable patients who had recovered from scrub typhus.Blood specimens were available six with typhus at 1 to 18 months after onset illness.The EDTA-treated blood inoculated into ECV304 cells, and cultures maintained for 7 months.Sequencing 56-kDa type-specific antigen gene O. was performed ascertain homology isolates.O. isolated all patients, nucleotide sequences isolates serially collected each patient identical...
To confirm that Korean Food and Drug Administration (KFDA) guidelines are applicable to test the efficacy of mosquito repellents, these were used complete protection times (CPTs) three representative repellents: N,N-diethyl-3-methylbenzamide (DEET), citronella, fennel oil. The repellency citronella oil decreased over time, from 97.9% at 0 h 71.4% 1 57.7% 2 h, as did oil, 88.6% 61.2% 47.4% h. In contrast, DEET remained 90% for 6 CPT (360 min) was much longer than CPTs (10.5 (8.4 min). These...
The cholesterol-lowering and anti-atherogenic effects of lemon essential oil (LEO) were investigated compared with the limonene. Owing to their volatility, both LEO limonene microencapsulated before preparation diet (20%, w/w). Hypercholesterolemia-induced rabbits divided into 3 groups based on plasma total cholesterol (TC) levels fed coating matrix (control group), (LEO or (Limonene group) for 8 weeks. dose-dependently inhibited low-density lipoprotein oxidation in vitro. Plasma TC lowest...
The interaction between neutrophils and endothelial cells is critical for the pathogenesis of vascular inflammation. However, regulation neutrophil adhesive function remains not fully understood. Intravital microscopy demonstrates that DREAM promotes recruitment to sites inflammation induced by TNF-α but MIP-2 or fMLP. We observe represses expression A20, a negative regulator NF-κB activity, enhances pro-inflammatory molecules phosphorylation IκB kinase (IKK) after stimulation. Studies using...
The lungs undergo constant mechanical strain as part of their function. Within the lung alveoli, alveolar epithelial type 1 (AT1) cells and associated capillary endothelial (CAP) comprise minimal gas exchange unit. How in niche respond to respiration-mediated forces remains poorly understood. Using a reversible bronchial ligation model control unilateral motion mice, we show that blocking respiratory movements leads transcriptional changes signaling involved dysfunctional repair. Inhibited...
The protective effects of a chondroitin sulfate-rich extract (CSE) from skate cartilage against lipopolysaccharide (LPS)-induced hepatic damage were investigated, and its mechanism action was compared with that sulfate (CS) shark cartilage. ICR mice orally administrated 200 mg/kg body weight (BW) CS or 400 BW CSE for 3 consecutive days, followed by one-time intraperitoneal injection LPS (20 BW). experimental groups vehicle treatment without (NC group), (LPS pretreatment (CS (CSE group)....
본 연구는 65세 이상 노인을 대상으로 고나트륨 섭취군을 분류하여 노인들의 식습관 개선 및 영양교육을 위한 사전연구로서 이들의 영양지식, 식습관, 식품섭취패턴을 조사하였다. 노인 135명의 조사대상자 중 42.96%인 58명이 섭취군에 속하였다. 생활습관에서 음주, 간식횟수, 외식횟수, 수분섭취량에서 섭취군이 유의적으로 높게 나타났으며, 자반류, 젓갈류, 장아찌, 조림류, 국물 섭취량, 간장과 소금의 추가량에 관한 점수가 섭취군에서 높았다. 섭취군의 나트륨에 영양지식 총점이 남녀노인 모두 낮았으며, 남자노인은 나트륨과 인스턴트식품, 인공조미료에 문항에서, 여자노인은 질병과의 관계, 나트륨 섭취권장량에서 낮았다. Na index 14의 김치찌개, 된장찌개, 미역국, 멸치볶음에서의 섭취빈도가 또한 인스턴트식품을 통한 총 섭취량은 남자노인 1562.49 mg, 여자노인 1227.82 mg으로 유의하게 높았으며, 특히 면류를 섭취량이 70% 이상이었다. 높을수록 14...
Neisseria meningitidis is a leading cause of bacterial meningitis in young adults. University students, especially those living dormitories, have been known to be at increased risk meningococcal disease. We performed longitudinal study determine the carriage rates N. and changes thereof.We recruited Inha freshmen who were, that time, admitted student dormitory. A pharyngeal swab was taken from all participant were also asked complete questionnaire. This repeated four weeks later.A total 136...
Although cell density is known to affect numerous biological processes including gene expression and fate specification, mechanistic understanding of what factors link global regulation lacking. Here, we reveal that the thousands genes in mouse embryonic stem cells (mESCs) affected by seeding low enhances efficiency differentiation. Mechanistically, β-catenin localized primarily adherens junctions during both self-renewal differentiation at high density. However, when mESCs differentiate...
Malonate utilization, an important differential trait, well recognized as being possessed by six of the seven Cronobacter species is thought to be largely absent in sakazakii (Csak). The current study provides experimental evidence that confirms presence a malonate utilization operon 24 strains sequence type (ST) 64, obtained from Europe, Middle East, China, and USA; it offers explanations regarding genomic diversity phylogenetic relatedness among these strains, other C. strains.In this...
문어, 오징어, 전복 및 주꾸미를 첨가하여 담근 수산물 첨가 김치와 일반 배추김치를 5℃에서 8주간 저장하면서 김치의 관능성 이화학적 특성을 관찰하였다. pH와 산도는 배추김치와 같이 정상 발효 패턴을 나타내었다. 저장기간 중 pH는 배추김치에 비해 높았고 이에 반해 김치가 배추김치보다 높았다. 젖산균 최대 균수는 Lactobacillus spp. 8.31~8.85 log CFU/mL, Leuconostoc 7.60~8.14 CFU/mL로 높았는데, 이는 환원당 함량이 높았기 때문이다. 적숙기(pH 4.3±0.1, 산도 0.7±0.1%)에 도달하는 시기는 배추김치, 전복김치 주꾸미김치가 저장 2주째, 문어김치 오징어김치가 3주째로 나타났다. 과숙기(pH 4.0 이하, 1.0% 이상)는 오징어김치(저장 6주째)를 제외한 모든 군이 8주째로 과숙기의 배추김치가 가장 낮았다. 적숙기 관능평가는 객관적 평가의 경우 신맛, 신내 탄산미가 유의적으로 낮게 평가되었다. 기호도 평가 항목...
Bartonella henselae causes cat-scratch disease, bacteremia, and various focal infections.Despite the worldwide occurrence of B. infections, reports in humans are rare Korea.The clinical manifestation all 5 previously reported cases was lymphadenopathy.Herein, we report a case bacteremia woman who presented with prolonged fever.B. isolated from blood specimen by cell culture.Conventional polymerase chain reaction amplification sequencing 16S-23S rRNA intergenic space region confirmed isolate...
The MYC proto-oncogene (MYC) is one of the most frequently overexpressed genes in breast cancer that drives stem cell-like traits, resulting aggressive disease progression and poor prognosis. In this study, we identified zinc finger transcription factor 148 (ZNF148, also called Zfp148 ZBP-89) as a direct target MYC. ZNF148 suppressed cell proliferation migration was transcriptionally repressed by cancer. Depletion short hairpin RNA (shRNA) CRISPR/Cas9 increased triple-negative (TNBC)...
This study examined the effect of a podophyllotoxin derivative, deoxypodophyllotoxin (anthricin), which is medicinal herb product isolated from Anthriscus sylvestris Hoffm. Deoxypodophyllotoxin was tested in rat PCA (passive cutaneous anaphylaxis) assay by administering intraperitoneally (1.0 to 10 mg/kg, i. p.) and intravenously (0.25 1.0 v.). dose-dependently inhibited reaction activated anti-dinitrophenyl (DNP) IgE. The inhibitory activity stronger than those prednisolone indomethacin,...