A5013019058- Cell Image Analysis Techniques
- Immune Response and Inflammation
- Immune cells in cancer
- Spectroscopy Techniques in Biomedical and Chemical Research
- Image Processing Techniques and Applications
- Influenza Virus Research Studies
- Single-cell and spatial transcriptomics
- Immune Cell Function and Interaction
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Photoacoustic and Ultrasonic Imaging
- Immunodeficiency and Autoimmune Disorders
- Asthma and respiratory diseases
- Immunotherapy and Immune Responses
- Embedded Systems Design Techniques
- Holomorphic and Operator Theory
- Bioinformatics and Genomic Networks
- Autoimmune and Inflammatory Disorders
- T-cell and B-cell Immunology
- Wound Healing and Treatments
- Bacterial Infections and Vaccines
- Railway Systems and Energy Efficiency
- Circadian rhythm and melatonin
- Advanced Biosensing Techniques and Applications
- Respiratory viral infections research
- Matrix Theory and Algorithms
Spanish National Centre for Cardiovascular Research
2020-2023
Universidad de Extremadura
2023
Università della Svizzera italiana
2017-2021
University of Bern
2018-2019
The mechanism by which inflammation influences the adaptive response to vaccines is not fully understood. Here, we examine role of lymph node macrophages (LNMs) in induction cytokine storm triggered inactivated influenza virus vaccine. Following vaccination, LNMs undergo inflammasome-independent necrosis-like death that reliant on MyD88 and Toll-like receptor 7 (TLR7) expression releases pre-stored interleukin-1α (IL-1α). Furthermore, activated medullary produce interferon-β (IFN-β) induces...
The role of natural killer (NK) cells in the immune response against vaccines is not fully understood. Here, we examine function infiltrated NK initiation inflammatory triggered by inactivated influenza virus vaccine draining lymph node (LN). We observed that, following vaccination, are recruited to interfollicular and medullary areas LN become activated type I interferons (IFNs) produced macrophages. activation leads their early production IFNγ, which turn regulates recruitment IL-6+ CD11b+...
The innate immune response generated against influenza infection is critical for the inhibition of viral dissemination. trachea contains different types cells that protect respiratory tract from pathogen invasion. Among them, γδ T have ability to rapidly generate large amounts pro-inflammatory cytokines preserve mucosal barrier homeostasis during infection. However, little known about their role early phase in airways. In this study, we found that, after infection, are recruited and...
Neutrophils are amongst the first cells to respond inflammation and infection. Although they play a key role in limiting dissemination of pathogens, study their dynamic behavior immune organs remains elusive. In this work, we characterized vivo neutrophils mouse popliteal lymph node (PLN) after influenza vaccination with UV-inactivated virus. To achieve this, used an image-based systems biology approach detect motility patterns associate them distinct actions. We described prominent rapid...
Abstract Recent advances in intravital video microscopy have allowed the visualization of leukocyte behavior vivo , revealing unprecedented spatiotemporal dynamics immune cell interaction. However, state-of-the-art software and methods for automatically measuring migration exhibit limitations tracking position leukocytes over time. Challenges arise both from complex patterns these cells experimental artifacts introduced during image acquisition. Additionally, development novel tools is...
Cell detection and tracking applied to in vivo fluorescence microscopy has become an essential tool biomedicine characterize 4D (3D space plus time) biological processes at the cellular level. Traditional approaches cell motion analysis by imaging, although based on automatic frameworks, still require manual supervision some points of system. Hence, when dealing with a large amount data, becomes incredibly time-consuming typically yields poor information. In this paper, we propose...
Intravital microscopy has revolutionized live-cell imaging by allowing the study of spatial–temporal cell dynamics in living animals. However, complexity data generated this technology limited development effective computational tools to identify and quantify processes. Amongst them, apoptosis is a crucial form regulated death involved tissue homeostasis host defense. Live-cell enabled at cellular level, enhancing our understanding its regulation. present, no method can deliver robust...
Abstract Neutrophils are innate immune cells involved in the elimination of pathogens and can also induce adaptive responses. Nα Nβ neutrophils have been described with distinct vitro capacity to generate antigen-specific CD8 T-cell However, how these cell types exert their role vivo manipulation Nβ/Nα ratio influences vaccine-mediated responses not known. In this study, we find that neutrophil subtypes show migratory motility patterns different ability interact T spleen following vaccinia...
The analysis of cell-cell or cell-pathogen interaction in vivo is an important tool to understand the dynamics immune response infection. Two-photon intravital microscopy (2P-IVM) allows observation cell interactions deep tissue living animals, while minimizing photobleaching generated during image acquisition. To date, different models for 2P-IVM lymphoid and non-lymphoid organs have been described. However, imaging respiratory remains a challenge due movement associated with breathing...
Intravital microscopy has revolutionized live-cell imaging by allowing the study of spatial–temporal cell dynamics in living animals. However, complexity data generated this technology limited development effective computational tools to identify and quantify processes. Amongst them, apoptosis is a crucial form regulated death involved tissue homeostasis host defense. Live-cell enabled at cellular level, enhancing our understanding its regulation. present, no method can deliver robust...
The analysis of cell-cell or cell-pathogen interaction in vivo is an important tool to understand the dynamics immune response infection.Two-photon intravital microscopy (2P-IVM) allows observation cell interactions deep tissue living animals, while minimizing photobleaching generated during image acquisition.To date, different models for 2P-IVM lymphoid and non-lymphoid organs have been described.However, imaging respiratory remains a challenge due movement associated with breathing cycle...
Intravital microscopy has revolutionized live cell imaging by allowing the study of spatial-temporal dynamics in living animals. However, complexity data generated this technology limited development effective computational tools to identify and quantify processes. Amongst them, apoptosis is a crucial form regulated death involved tissue homeostasis host defense. Live-cell enabled at cellular level, enhancing our understanding its regulation. present, no method can deliver robust detection...
Abstract Intravital microscopy has revolutionized live cell imaging by allowing the study of spatial-temporal dynamics in living animals. However, complexity data generated this technology limited development effective computational tools to identify and quantify processes. Amongst them, apoptosis is a crucial form regulated death involved tissue homeostasis host defense. Live-cell enabled at cellular level, enhancing our understanding its regulation. present, no method can deliver robust...
Intravital microscopy has revolutionized live cell imaging by allowing the study of spatial-temporal dynamics in living animals. However, complexity data generated this technology limited development effective computational tools to identify and quantify processes. Amongst them, apoptosis is a crucial form regulated death involved tissue homeostasis host defense. Live-cell enabled at cellular level, enhancing our understanding its regulation. present, no method can deliver label-free...
Identifying the most relevant variables or features in massive datasets for dimensionality reduction can lead to improved and more informative display, faster computation times, explainable models of complex systems. Despite significant advances available algorithms, this task generally remains challenging, especially unsupervised settings. In work, we propose a method that constructs correlation networks using all intervening then selects ones based on network bootstrapping. The be applied...