A5079207369- Cancer Genomics and Diagnostics
- Neonatal and Maternal Infections
- Bacterial Infections and Vaccines
- Genetic factors in colorectal cancer
- Hepatocellular Carcinoma Treatment and Prognosis
- Neonatal and fetal brain pathology
- Preterm Birth and Chorioamnionitis
- CRISPR and Genetic Engineering
- Immunotherapy and Immune Responses
- Cancer, Lipids, and Metabolism
- CAR-T cell therapy research
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Lung Cancer Treatments and Mutations
- Biosensors and Analytical Detection
- Nanoplatforms for cancer theranostics
- Malaria Research and Control
- RNA Research and Splicing
- Mosquito-borne diseases and control
- MicroRNA in disease regulation
- Cancer Immunotherapy and Biomarkers
- Circular RNAs in diseases
- Radiomics and Machine Learning in Medical Imaging
- COVID-19 Impact on Reproduction
Mengchao Hepatobiliary Hospital
2018-2025
Fujian Medical University
2018-2025
Fuzhou University
2019-2024
Circulating tumor DNA (ctDNA) provides a novel approach for detecting burden and predicting clinical outcomes of hepatocellular carcinoma (HCC). Here, we performed thorough evaluation HCC circulating genetic features further fully integrated them to build robust strategy monitoring prognostic outcome assessment.We target sequencing low-coverage whole-genome on plasma samples collected from 34 long-term follow-up patients with capture somatic SNVs CNVs, respectively. Clinical information was...
Circular RNA (circRNA) can participate in various biological processes, including tumorigenesis, through their microRNA response elements. Alterations circRNA profiles during hepatocellular carcinoma (HCC) progression and clinical significance remain unclear. Here, we present extensive analysis of tumor matched peritumor tissues collected from 10 HCC patients, conducted to identify related progression. A total 42 dysregulated (38 down-regulated 4 up-regulated) were identified compared with...
Abstract Growing evidences have proved that tumors evade recognition and attack by the immune system through escape mechanisms, PDL1/Pbrm1 genes a strong correlation with poor response or resistance to checkpoint blockade (ICB) therapy. Herein, multifunctional biomimetic nanocarrier (siRNA‐CaP@PD1‐NVs) is developed, which can not only enhance cytotoxic activity of cells blocking PD1/PDL1 axis, but also reduce tumor via Pbrm1/PDL1 gene silencing, leading significant improvement in...
Aberrant RNA editing, mediated by adenosine deaminases acting on (ADAR), serves as a post-transcriptional event participating in tumorigenesis and prognosis. However, the editing profiles during HCC progression their clinical correlations remain unclear.Multiple tissue samples were collected from an advanced patient. RNA-seq was performed to obtain for each sample. Two sites CDK13 further validated 60 patients; potential regulatory mechanisms investigated.In-depth analysis of data revealed...
Abstract Background Streptococcus agalactiae or group B (GBS) is a leading infectious cause of neonatal morbidity and mortality. It essential to establish robust method for the rapid ultra-sensitive detection GBS in pregnant women with premature rupture membrane (PROM). Methods This study developed CRISPR-GBS assay that combined advantages recombinase polymerase amplification (RPA) CRISPR/Cas12a system detection. The clinical performance was assessed using vaginal cervical swabs were...
Tumor-infiltrating CD8+ T cells and programmed death-1 (PD1) levels are critical indicators for tumor immunophenotyping therapeutic decision-making. Noninvasive optical imaging in the second near infrared window (NIR-II) is particularly well-suited investigating biological processes within tumors live mammals, thanks to its deep-tissue penetration superior spatiotemporal resolution. However, vivo NIR-II has primarily been restricted a single probe at time. Methods: Herein, we developed...
As key players in HCC antitumor response, the functions of tumor infiltrated CD8+ T cells are significantly affected by surrounding microenvironment. A detailed profiling their genomic and transcriptional changes could provide valuable insights for both future immunotherapy development prognosis evaluation. We performed whole exome transcriptome sequencing on isolated from other tissue origins (peritumor tissues corresponding PBMCs) eight treatment-naive patients. The results demonstrated...
To investigate tumor clonal evolution in hepatocellular carcinoma (HCC), we collected 31 samples,16 peritumor samples and matched PBMCs from 11 long‐term follow‐up patients with HCC. Whole‐exome sequencing was performed to obtain SNVs CNVs for each sample. An average of 652.2 somatic mutations were identified patient the mean percentage nonubiquitous 63.7% (range, 0.7%–100%), reflecting variety heterogeneity. Further analysis conducted based on mutation clustering results revealed that...
Abstract The long reads of Nanopore sequencing permit accurate transcript assembly and ease in discovering novel transcripts with potentially important functions cancers. wide adoption for quantification, however, is largely limited by high costs. To address this issue, we developed a bioinformatics software, NovelQuant, that can specifically quantify long‐read‐assembled short‐read data. Direct RNA Sequencing was carried out on three hepatocellular carcinoma (HCC) patients’ tumor, matched...
Circular RNAs (circRNAs) could interact with miRNAs to regulate gene expression, participating in hepatocellular carcinoma (HCC) initiation and development. This work aimed determine the potential function molecular mechanism of circEPB41L2 (hsa_circ_0077837) during HCC progression.The expression tissues cell lines was quantified using real-time quantitative PCR (qRT-PCR). CCK-8 assays colony formation were utilized detect proliferation cells. Wound healing assay transwell performed...
Currently, malaria is still one of the major public health problems commonly caused by four Plasmodium species. The similar symptoms and COVID-19 epidemic fever or fatigue lead to frequent misdiagnosis. disadvantages existing detection methods, such as time-consuming, costly, complicated operation, need for experienced technicians, indistinguishable typing, difficulties in meeting clinical requirements rapid, easy, accurate typing common In this study, we developed optimized a universal...
Abstract The immune status of tumor‐infiltrating lymphocytes (TILs) is essential for the effectiveness cancer immunotherapies. However, due to diversity in TILs, cellular heterogeneity, and applicability clinic, it still lacking effective strategies meet clinical needs. We developed a novel immuno‐recognition‐induced method based on rolling circle amplification (RCA), namely immunoRCA, situ visualize TILs actual samples. This immunoRCA method, which, feature mRNAs were used as biomarkers has...
<p>Figure S2. The concordance of somatic SNVs detected in preoperative plasma sample and matched tumor enrolled patients. A. bar plot demonstrating the R2 Pearson correlation between SNVs' mutant allele frequency corresponding tissues. B. representative scatter plots showing whole exome sequencing target sequencing. Each SNV is represented as a single circle.</p>
<p>Figure S5. Kaplan-Meier analysis demonstrating RFS and OS for all enrolled patients stratified by their status of follow up ctDNA.</p>
<p>Figure S3. CNVs levels between preoperative plasma samples and matched tumor in enrolled patients. The genome-wide copy number plots showed the correlation of tumor. calculated CNV fractions were also provided. Only patients with fraction>0 sample shown.</p>
<p>Figure S1. The distribution of known driver genes' somatic SNVs in enrolled patient.</p>
<p>Clinical characteristics of 34 enrolled HCC patients.</p>
<p>Figure S4.The relationship between SNV fraction, CNV fraction and clinical features. A. The scatter plot showed the Pearson correlation fraction. B. Tumor size C. D. box differences of in patient groups with without microvascular invasion using students' t test. E. different level tumor differentiation F. or recurrence G. H. I. test.</p>
<p>Clinical characteristics of 34 enrolled HCC patients.</p>