A5023355464- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Hepatocellular Carcinoma Treatment and Prognosis
- Genetic factors in colorectal cancer
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Cancer-related molecular mechanisms research
- Lung Cancer Treatments and Mutations
- Cancer, Lipids, and Metabolism
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- CAR-T cell therapy research
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Atherosclerosis and Cardiovascular Diseases
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- Biosensors and Analytical Detection
- Radiomics and Machine Learning in Medical Imaging
- Advanced biosensing and bioanalysis techniques
- Wnt/β-catenin signaling in development and cancer
- CRISPR and Genetic Engineering
- Immune cells in cancer
- Inflammatory Biomarkers in Disease Prognosis
- Extracellular vesicles in disease
Mengchao Hepatobiliary Hospital
2018-2025
Fujian Medical University
2018-2025
Fuzhou University
2019-2024
Fujian University of Traditional Chinese Medicine
2016-2019
Shanghai East Hospital
2016
Tongji University
2016
Fudan University
2016
State Key Laboratory of Genetic Engineering
2016
Shanghai Changzheng Hospital
2016
Circulating tumor DNA (ctDNA) provides a novel approach for detecting burden and predicting clinical outcomes of hepatocellular carcinoma (HCC). Here, we performed thorough evaluation HCC circulating genetic features further fully integrated them to build robust strategy monitoring prognostic outcome assessment.We target sequencing low-coverage whole-genome on plasma samples collected from 34 long-term follow-up patients with capture somatic SNVs CNVs, respectively. Clinical information was...
Circular RNA (circRNA) can participate in various biological processes, including tumorigenesis, through their microRNA response elements. Alterations circRNA profiles during hepatocellular carcinoma (HCC) progression and clinical significance remain unclear. Here, we present extensive analysis of tumor matched peritumor tissues collected from 10 HCC patients, conducted to identify related progression. A total 42 dysregulated (38 down-regulated 4 up-regulated) were identified compared with...
Abstract Background Clinically, prophylactic anti-recurrence treatments for hepatocellular carcinoma (HCC) patients after radical surgery are extremely limited. Neoantigen based vaccine can generate robust anti-tumor immune response in several solid tumors but whether it could induce HCC and serve as a safe effective strategy preventing postoperative recurrence still remain largely unclear. Methods Personalized neoantigen was designed immunized 10 with high risk of prime-boost schedule. The...
Personalized neoantigen vaccine could induce a robust antitumor immune response in multiple cancers, whose efficacy be further enhanced by combining with programmed cell death 1 blockade (α-PD-1). However, the corresponding and synergistic mechanisms remain largely unclear. Here, we aimed to develop clinically available combinational therapeutic strategy explore its potential hepatocellular carcinoma (HCC).Neoantigen peptide (NeoVAC) for murine HCC line Hepa1-6 was developed optimized...
Cell survival rate determines engraftment efficiency in adipose-derived mesenchymal stem cell (ADSC)-based regenerative medicine. In vivo monitoring of ADSC viability to achieve effective tissue regeneration is a major challenge for therapy. Here, we developed an activated near-infrared II (NIR-II) fluorescent nanoparticle consisting lanthanide-based down-conversion nanoparticles (DCNPs) and IR786s (DCNP@IR786s) labeling real-time tracking vivo. dying ADSCs due excessive ROS generation,...
Background: Myocardial infarction is one of the leading causes morbidity and mortality worldwide, triggering irreversible myocardial cell damage heart failure. The role low-density lipoprotein receptor-related proteins 5 6 (LRP5/6) as coreceptors Wnt/β-catenin pathway in adult remain unknown. Insulin-like growth factor binding protein 4 dickkopf-related 1 (Dkk1) are 2 secreted LRP5/6 that play a crucial development through preventing activation. However, their roles unexplored. Methods: To...
Abstract The RNA-binding protein PNO1 is critical for ribosome biogenesis, but its potential role in cancer remains unknown. In this study, online data mining, cDNA, and tissue microarrays indicated that expression was higher colorectal than noncancerous tissue, overexpression associated with worse patient survival. Gain-of-function loss-of-function studies demonstrated knockdown suppressed growth of cells vitro vivo, while promoted cell proliferation vitro. expressing wild-type p53,...
Aberrant RNA editing, mediated by adenosine deaminases acting on (ADAR), serves as a post-transcriptional event participating in tumorigenesis and prognosis. However, the editing profiles during HCC progression their clinical correlations remain unclear.Multiple tissue samples were collected from an advanced patient. RNA-seq was performed to obtain for each sample. Two sites CDK13 further validated 60 patients; potential regulatory mechanisms investigated.In-depth analysis of data revealed...
Background Collecting duct carcinoma (CDC) of the kidney is a rare and highly aggressive malignant tumor with worst prognosis among all renal cancers. Nevertheless, first-line treatments, including chemotherapy target therapy, usually show poor response to CDC. Recent studies have suggested that immunotherapy targeting personal tumor-specific neoantigens could be promising strategy for several solid However, whether it has therapeutic potential in CDC remains unclear. Case presentation Here,...
While the value of Des-γ-carboxy prothrombin in hepatocellular carcinoma diagnosis has been widely acknowledged, whether or how could be used recurrence evaluation remains largely unexplored. We performed a multicenter retrospective analysis including an Exploration Cohort (1074 patients, 5133 measurements) and Validation (263 612 to investigate evaluate patients' prognosis. introduced dynamic rate as normalized quantitative measurement change. rates were further applied high-risk liver...
Baicalin is one of the active ingredients extracted from dry root Scutellaria baicalensis Georgi, which has been reported to be effective in preventing myocardial ischemia reperfusion injury. However, mechanisms underlying its cardioprotective activities remain elucidated. In present study, H2O2‑treated cardiomyocyte H9c2 cell line served as an vitro model oxidation‑damaged cardiomyocytes evaluate effects baicalin on cardiac injury, and investigate molecular mechanism. The results...
As key players in HCC antitumor response, the functions of tumor infiltrated CD8+ T cells are significantly affected by surrounding microenvironment. A detailed profiling their genomic and transcriptional changes could provide valuable insights for both future immunotherapy development prognosis evaluation. We performed whole exome transcriptome sequencing on isolated from other tissue origins (peritumor tissues corresponding PBMCs) eight treatment-naive patients. The results demonstrated...
Neoantigens derived from tumor-specific genomic alterations have demonstrated great potential for immunotherapeutic interventions in cancers. However, the comprehensive profile of hepatocellular carcinoma (HCC) neoantigens and their complex interplay with immune microenvironment tumor evolution not been fully addressed.Here we integrated whole exome sequencing data, transcriptome data clinical information 72 primary HCC patients to characterize neoantigen profile, systematically explored its...
Abstract The long reads of Nanopore sequencing permit accurate transcript assembly and ease in discovering novel transcripts with potentially important functions cancers. wide adoption for quantification, however, is largely limited by high costs. To address this issue, we developed a bioinformatics software, NovelQuant, that can specifically quantify long‐read‐assembled short‐read data. Direct RNA Sequencing was carried out on three hepatocellular carcinoma (HCC) patients’ tumor, matched...
Circular RNAs (circRNAs) could interact with miRNAs to regulate gene expression, participating in hepatocellular carcinoma (HCC) initiation and development. This work aimed determine the potential function molecular mechanism of circEPB41L2 (hsa_circ_0077837) during HCC progression.The expression tissues cell lines was quantified using real-time quantitative PCR (qRT-PCR). CCK-8 assays colony formation were utilized detect proliferation cells. Wound healing assay transwell performed...
Transarterial chemoembolization (TACE) is the most commonly used treatment for advanced hepatocellular carcinoma (HCC), but still lacks accurate real-time biomarkers monitoring its therapeutic efficacy. Here, we explored whether copy number profiling of circulating free DNA (cfDNA) could be utilized to predict responses and prognosis in HCC patients with TACE treatment. In total, 266 plasma cfDNA samples were collected from 64 patients, 57 liver cirrhosis (LC) 32 healthy volunteers. We...
To overcome the dependency of strategies utilizing cell-free DNA (cfDNA) on tissue sampling, emergence sequencing panels for non-invasive mutation screening was promoted. However, cfDNA with still suffers from either inaccuracy or omission, and novel approaches accurately tumor mutations solely based plasma without gene panel restriction are urgently needed. We performed unique molecular identifier (UMI) target samples peripheral blood mononuclear cells (PBMCs) 85 hepatocellular carcinoma...
Abstract CircRNAs have been reported to play crucial roles in tumor progression and recurrence, showing potential as biomarkers cancer. However, the global abundance of circRNA their involvement hepatocellular carcinoma (HCC) development not fully explored. Whole transcriptome sequencing was performed on peritumor from 60 patients with HCC quantify expression circRNAs, calculated by index (CRI). Gene‐set enrichment analysis weighted gene co‐expression network were used reveal biological...
<div>Abstract<p>The RNA-binding protein PNO1 is critical for ribosome biogenesis, but its potential role in cancer remains unknown. In this study, online data mining, cDNA, and tissue microarrays indicated that expression was higher colorectal than noncancerous tissue, overexpression associated with worse patient survival. Gain-of-function loss-of-function studies demonstrated knockdown suppressed growth of cells <i>in vitro</i> vivo</i>, while promoted cell...
<p>Figure S2 indicates that higher PNO1 mRNA levels are associated with poorer overall survival, relapse-free survival and shorter time of first progression patients breast or lung cancer. knockdown decreased cell viability cancer cells.</p>