A5053189930- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Diabetes Treatment and Management
- Genetic and Kidney Cyst Diseases
- SARS-CoV-2 and COVID-19 Research
- Liver Disease Diagnosis and Treatment
- Diabetes Management and Research
- COVID-19 Clinical Research Studies
- Biochemical and Molecular Research
- Genetic Syndromes and Imprinting
- Long-Term Effects of COVID-19
- Diet, Metabolism, and Disease
- Adipose Tissue and Metabolism
- Diabetes and associated disorders
Instituto de Biomedicina y Genética Molecular de Valladolid
2020-2025
Consejo Superior de Investigaciones Científicas
2022-2025
Universidad de Valladolid
2020-2025
The insulin-degrading enzyme (IDE) is a metalloendopeptidase with high affinity for insulin. Human genetic polymorphisms in Ide have been linked to increased risk T2DM. In mice, hepatic ablation causes glucose intolerance and insulin resistance when mice are fed regular diet.ObjectiveThese studies were undertaken further investigate its regulatory role homeostasis sensitivity diet-induced obesity.MethodsTo this end, we compared the metabolic effects of loss versus gain IDE function high-fat...
Type 2 diabetes is characterised by hyperglucagonaemia and perturbed function of pancreatic glucagon-secreting alpha cells but the molecular mechanisms contributing to these phenotypes are poorly understood. Insulin-degrading enzyme (IDE) present within all islet cells, mostly in both mice humans. Furthermore, IDE can degrade glucagon as well insulin, suggesting that may play an important role cell vivo.We have generated a novel mouse model with cell-specific deletion Ide, A-IDE-KO line....
Insulin-degrading enzyme (IDE) is a highly conserved and ubiquitously expressed Zn2+-metallopeptidase that regulates hepatic insulin sensitivity, albeit its regulation in response to the fasting-to-postprandial transition poorly understood. In this work, we studied of IDE mRNA protein levels as well proteolytic activity liver, skeletal muscle, kidneys under fasting (18 h) refeeding (30 min 3 conditions, mice fed standard (SD) or high-fat (HFD) diets. liver an HFD, reduced (~30%); whereas...
Although the COVID-19 disease has developed into a worldwide pandemic, its pathophysiology remains to be fully understood. Insulin-degrading enzyme (IDE), zinc-metalloprotease with high affinity for insulin, been found in interactomes of multiple SARS-CoV-2 proteins. However, relevance IDE innate and adaptative immune responses elicited by circulating peripheral blood mononuclear cells is unknown. Here, we show that highly expressed on surface monocytes, T-cells (both CD4+ CD4-), and, lower...
IDE is a ubiquitous metalloprotease with cytosolic and mitochondrial subcellular localization that degrades insulin glucagon. People T2D diet-induced obese mice show lower hepatic levels. We revealed key role of on the insulin-mediated repression gluconeogenesis, but its function glucagon-dependent activation gluconeogenesis respiration in hepatocytes remains completely unknown. Here, we aim to elucidate glucagon signalling impact energy metabolism hepatocytes. Liver homogenized primary...