- Fermentation and Sensory Analysis
- Phytochemicals and Antioxidant Activities
- Horticultural and Viticultural Research
- Metabolomics and Mass Spectrometry Studies
- Tea Polyphenols and Effects
- Biochemical Analysis and Sensing Techniques
- Biochemical and biochemical processes
- African Botany and Ecology Studies
- Food Chemistry and Fat Analysis
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Meat and Animal Product Quality
- Analytical Chemistry and Chromatography
- Tannin, Tannase and Anticancer Activities
- Phytochemistry and Biological Activities
- Microbial Metabolites in Food Biotechnology
- Antioxidant Activity and Oxidative Stress
- Essential Oils and Antimicrobial Activity
- Protein Hydrolysis and Bioactive Peptides
- Polysaccharides and Plant Cell Walls
- Advanced Chemical Sensor Technologies
- Nuts composition and effects
- Chromatography in Natural Products
- Food Quality and Safety Studies
- Biochemical effects in animals
Sciences pour L’Œnologie
2016-2025
Institut d'Etudes Politiques de Paris
2015-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Université de Montpellier
2009-2025
Institut Agro Montpellier
2011-2025
L'Institut Agro
2022-2024
Institut Charles Gerhardt Montpellier
2017
Institut de Chimie
2017
Institut National de la Recherche Agronomique du Niger
2014
Institut National de la Recherche Agronomique
2003-2011
Tannins are believed to be particularly abundant in tropical tree foliage and mainly associated with plant herbivore defense. Very little is known of the quantity, variation, potential role tannins leaf litter. Here we report on interspecific variability litter condensed tannin (CT) concentration among 16 co‐occurring rain forest species French Guiana explore functional significance variable CT for decomposition. We compared some classical methods ecological literature a method based...
Abstract Anthocyanins are major pigments in colored grape (Vitis vinifera) berries, and most of them monomethoxylated or dimethoxylated. We report here the functional characterization an anthocyanin O-methyltransferase (AOMT) from grapevine. The expression pattern two cultivars with different methylation profiles (Syrah Nebbiolo) showed a peak at start ripening (véraison), when concentrations all methylated anthocyanins begin to increase. purified recombinant AOMT protein was active on both...
Date fruit seeds have been demonstrated to possess high antioxidant activities due their content of flavonoids and phenolic compounds. The objective this work was identify quantify the composition date seeds.Two UPLC-DAD-ESI-MS analyses were performed on seed Khalas variety as follows: (1) an analysis simple compounds [phenolic acids, hydroxycinnamic flavonols, flavones, flavan-3-ols (monomers, dimers trimers)]; (2) all proanthocyanidin oligomers polymers) after depolymerisation.The amount...
A rapid, sensitive and selective analysis method using Ultra High Performance Liquid Chromatography coupled to triple-quadrupole Mass Spectrometry (UHPLC-QqQ-MS) has been developed for the quantification of polyphenols in rosé wines. The compound detection being based on specific MS transitions Multiple Reaction Monitoring (MRM) mode, present allows up 152 phenolic two additional non-phenolic wine compounds 30 min without sample purification or pre-concentration, even at low concentration...
Phenolic compounds represent a large family of plant secondary metabolites, essential for the quality grape and wine playing major role in defense against biotic abiotic stresses. composition is genetically driven greatly affected by environmental factors, including water stress. A challenge breeding grapevine cultivars adapted to climate change with high potential wine-making dissect complex metabolic response involved adaptation mechanisms. targeted metabolomics approach based on ultra...
The reaction between procyanidin dimer Ec-EcG (B2 3'-O-gallate) and malvidin 3-O-glucoside (Mv3glc) was studied in a model solution system at two different pH values, 2.0 3.8. Disappearance of both species much faster 3.8 than 2.0. That Mv3glc increased the presence Ec-EcG, whereas that same or absence anthocyanin. Values absorbance 520 nm measured were correlated with amount residual Mv3glc. Those hardly changed during incubation, but values 420 620 as well resistance to sulfite bleaching...
A method based on high-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS) following fractionation by a Sephadex LH-20 column has been developed to determine the phenolic composition of fruit Eucalyptus globulus growing in Algeria. The presence 18 gallotannins, 26 ellagitannins, 2 flavonols was established. Tentative identification is provided for these compounds basis UV−visible spectra data. Most described...
In plants, the shikimate pathway provides aromatic amino acids that are used to generate numerous secondary metabolites, including phenolic compounds. this pathway, dehydrogenases (SDH) 'classically' catalyse reversible dehydrogenation of 3-dehydroshikimate shikimate. The capacity SDH produce gallic acid from metabolites has not been studied in depth. grapevine berries, mainly accumulates as galloylated flavan-3-ols. four proteins have produced Escherichia coli vitro, VvSDH1 exhibited...
Abstract Industrial processing of raspberries into juice and jam results in the production with high content lipophilic hydrophilic phytochemicals. Usually considered as waste, raspberry pomace is occasionally cold‐pressed to recover specialty oil. However, resulting press‐cake (PPC) still contains 30% 35% compounds, such essential fatty acids, tocols, phytosterols, ellagitannins initially present pomace. In a perspective sustainable development, we investigate an eco‐friendly process using...
The fractionation of a grape skin extract by multilayer countercurrent chromatography coupled with step gradient elution allowed the preparation fraction almost devoid free anthocyanins. This appeared to be exclusively polymeric, as judged liquid chromatographic−mass spectrometric (LC-MS) analysis, color-bleaching tests sulfur dioxide, and thiolysis. Electrospray mass analysis indicated that pigmented material in this was chiefly composed direct condensation products anthocyanin extending up...
The gamma-aminobutyrate (GABA) shunt, an alternative route for the conversion of alpha-ketoglutarate to succinate, involves glutamate decarboxylase Gad1p, GABA transaminase Uga1p and succinate semialdehyde dehydrogenase Uga2p. This pathway has been extensively described in plants animals, but its function yeast remains unclear. We show that flux through Gad1p is insignificant during fermentation rich sugar-containing medium, excluding a role this redox homeostasis under anaerobic conditions...
Summary Nitrogen‐fixing root nodulation is confined to four plant orders, including > 14 000 L eguminosae, one nonlegume genus P arasponia and c . 200 actinorhizal species that form symbioses with rhizobia F rankia bacterial species, respectively. Flavonoids have been identified as signals developmental regulators for in legumes long hypothesized play a critical role during nodulation. However, direct evidence of their involvement symbiosis lacking. Here, we used RNA interference silence...
Eucalyptus species are widely cultivated in Mediterranean regions. Moreover, plants of this family have been utilized for medicinal purposes. A number studies devoted to the identification eucalypt phenolics, all them focused on specific families compounds, and no exhaustive profiling has reported leaves plant.To develop methods that allows quantification different classes phenolics globulus leaf.Acetonic extract was fractionated by chromatography a Sephadex LH-20 column using consecutive...
Grape proanthocyanidins (PAs) play a major role in the organoleptic properties of wine. They are accumulated mainly grape skin and seeds during early stages berry development. Despite recent progress identification genes involved PA biosynthesis, mechanisms subunit condensation, galloylation, or fine regulation spatio-temporal composition berries PAs still not elucidated. Two Myb transcription factors, VvMybPA1 VvMybPA2, controlling pathway have recently been identified ectopically...
Direct addition of anthocyanins and flavan-3-ols was investigated in a model system by incubating malvidin 3-glucoside (-)-epicatechin ethanol. Analysis reaction products high-performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC/ESI-MS) before after thiolysis showed the formation colorless dimers detected at m/z 781 negative ion mode, with retention times spectroscopic characteristics identical those compounds wine, which contain one unit flavanol...
In sub-Saharan Africa, chronic malnutrition is often associated with intestinal inflammation and oxidative stress. African green leafy vegetables (GLVs), commonly consumed by these populations rich in bioactive compounds, may improve the antioxidant status. The aim of this study was to measure capacity using complementary assays (DPPH, FRAP, ABTS, ORAC NO scavenging) polar non-polar leaf extracts four GLVs, cassava (Manihot esculenta), roselle (Hibiscus sabdariffa), jute mallow (Corchorus...