A5053965636- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Insect symbiosis and bacterial influences
- Cytomegalovirus and herpesvirus research
- Innovation and Socioeconomic Development
- Enzyme Structure and Function
- Glycosylation and Glycoproteins Research
- Heat shock proteins research
- thermodynamics and calorimetric analyses
- Genetics, Bioinformatics, and Biomedical Research
- Animal Genetics and Reproduction
- Machine Learning in Bioinformatics
- Epigenetics and DNA Methylation
- Evolution and Genetic Dynamics
University of California, San Francisco
2019-2022
Indian Institute of Science Bangalore
2020-2022
Jawaharlal Nehru Centre for Advanced Scientific Research
2012-2022
Abstract The viruses that infect bacteria, bacteriophages (or phages), possess numerous genes of unknown function. Genetic tools are required to understand their biology and enhance efficacy as antimicrobials. Pseudomonas aeruginosa jumbo phage ΦKZ its relatives a broad host range family assemble proteinaceous “phage nucleus” structure during infection. Due the nucleus, DNA-targeting CRISPR-Cas is ineffective against this thus there currently no reverse genetic for family. Here, we develop...
SUMMARY Bacterial CRISPR-Cas systems employ RNA-guided nucleases to destroy foreign DNA. Bacteriophages, in turn, have evolved diverse “anti-CRISPR” proteins (Acrs) counteract acquired immunity. In Listeria monocytogenes , prophages encode 2-3 distinct anti-Cas9 proteins, with acrIIA1 always present; however, its mechanism is unknown. Here, we report that AcrIIA1 binds high affinity Cas9 via the catalytic HNH domain and, triggers degradation. displays broad-spectrum inhibition of Type II-A...
Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) systems are prokaryotic adaptive immune that have been well characterized biochemically, but in vivo spatiotemporal regulation and cell biology remain largely unaddressed. Here, we used fluorescent fusion proteins introduced at the chromosomal CRISPR-Cas locus to study localization of type I-F system Pseudomonas aeruginosa. When lacking a target cell, Cascade complex is broadly nucleoid bound, while...
Bacteria and bacteriophages have evolved DNA modification as a strategy to protect their genomes. Mom protein of bacteriophage Mu modifies the phage DNA, rendering it refractile numerous restriction enzymes in turn enabling successfully invade variety hosts. A strong fortification, combined activity host factors, prevents untimely expression mom associated toxic effects. Here, we identify bacterial chromatin architectural Fis an additional player this crowded regulatory cascade. Both vivo...
Abstract The broad host range bacteriophage Mu employs a novel ‘methylcarbamoyl’ modification to protect its DNA from diverse restriction systems of hosts. is catalyzed by phage-encoded protein Mom, whose mechanism action mystery. Here, we characterized the co-factor and metal-binding properties Mom provide molecular explain ‘methylcarbamoyl’ation Mom. Computational analyses revealed conserved GNAT (GCN5-related N-acetyltransferase) fold in We demonstrate that binds acetyl CoA identify...
Epigenetic modifications play diverse roles in biological systems. Nucleic acid control gene expression, protein synthesis, and sensitivity to nucleic acid-cleaving enzymes. However, the mechanisms underlying biosynthesis of can be challenging identify. Studying protein-ligand interactions helps decipher biosynthetic regulatory pathways reactions. Here, we describe a fluorescence labeling-based quantitative method for unraveling biomolecular bacteriophage Mu DNA modification Mom with its...
Abstract The broad host range bacteriophage Mu employs a novel ‘methylcarbamoyl’ modification to protect its DNA from diverse restriction systems. Biosynthesis of the unusual is longstanding mystery. Moreover, isolation Mom, phage protein involved in has remained elusive date. Here, we characterized co-factor and metal binding properties Mom provide molecular mechanism explain ‘methylcarbamoyl’ation by Mom. Our computational analyses revealed conserved GNAT (GCN5-related N-acetyltransferase)...