A5043443505- HIV Research and Treatment
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- HIV/AIDS drug development and treatment
- RNA modifications and cancer
- interferon and immune responses
- Viral Infections and Immunology Research
- Molecular Biology Techniques and Applications
- Nanopore and Nanochannel Transport Studies
- RNA regulation and disease
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Herpesvirus Infections and Treatments
- Mosquito-borne diseases and control
- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
- Influenza Virus Research Studies
- DNA and Nucleic Acid Chemistry
- Cytomegalovirus and herpesvirus research
- HIV/AIDS Research and Interventions
- Plant Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Phagocytosis and Immune Regulation
- Antimicrobial Peptides and Activities
- HIV-related health complications and treatments
Architecture et Réactivité de l'arN
2015-2025
Université de Strasbourg
2014-2025
Centre National de la Recherche Scientifique
2014-2025
Institut de Biologie Moléculaire et Cellulaire
2004-2020
Universidad del Sureste
2020
Science Applications International Corporation (United States)
2007
Institut de Chimie
2007
Institut de Biologie Moléculaire des Plantes
1994-2004
Dana-Farber Cancer Institute
1999
Harvard University
1999
The diploid genome of all retroviruses is made two homologous copies RNA intimately associated near their 5' end, in a region called the dimer linkage structure. Dimerization genomic thought to be important for crucial functions retroviral life cycle (reverse transcription, translation, encapsidation). Previous vitro studies mapped structure human immunodeficiency virus type 1 (HIV-1) downstream splice donor site, containing conserved purine tracts that were postulated mediate dimerization,...
RNA-RNA interactions govern a number of biological processes. Several RNAs, including natural sense and antisense interact by means two-step mechanism: recognition is mediated loop-loop complex, which then stabilized formation an extended intermolecular duplex. It was proposed that the same mechanism holds for dimerization genomic RNA human immunodeficiency virus type 1 (HIV-1), event thought to control crucial steps HIV-1 replication. However, whereas interaction between partially...
The genome of all retroviruses consists in two homologous RNA molecules associated near their 5' end i n a region called the dimer linkage structure.Dimerization genomic is thought to be important for several functions retroviral cycle such as encapsidation, reverse transcription, and translation.In human immunodeficiency virus type 1 (HIV-l), downstream splice donor site was initially postulated mediate dimerization.However, we recently showed that dimerization initiation located upstream...
In retroviruses, the genomic RNA is in form of a 60S-70S complex composed two identical genome-length molecules tightly associated through numerous interactions. A major interaction, called dimer linkage structure, has been found near 5' end and probably involved control translation, packaging, recombination during proviral DNA synthesis. Recently, small sequence corresponding to stem-loop structure located leader human immunodeficiency virus type 1 (HIV-1) was be required for initiation...
The influenza A virus genome consists of eight viral RNAs (vRNAs) that form ribonucleoproteins (vRNPs). Even though evidence supporting segment-specific packaging vRNAs is accumulating, the mechanism ensuring selective one copy each vRNA into particles remains largely unknown. We used electron tomography to show vRNPs emerge from a common 'transition zone' located underneath matrix layer at budding tip virions, where they appear be interconnected and often star-like structure. This zone...
Human immunodeficiency virus type-1 (HIV-1) is a complex retrovirus that relies on alternative splicing, translational, and post-translational mechanisms to produce over 15 functional proteins from its single ~10 kb transcriptional unit. Using ribosome profiling, nascent protein labeling, RNA sequencing, whole-proteomics of infected CD4 + T lymphocytes, we characterized the transcriptional, landscape during infection. While viral infection exerts significant impact host transcript abundance,...
ABSTRACT Targeting of the human immunodeficiency virus type 1 (HIV-1) Gag precursor Pr55 gag to plasma membrane, site assembly, is primarily mediated by N-terminal matrix (MA) domain. N-myristylation MA essential for stable association with membranes and assembly. We now show that single amino acid substitutions near N terminus can dramatically impair assembly without compromising myristylation. Subcellular fractionation demonstrated membrane binding was compromised a similar extent as in...
With the increasing interest of RNAs in regulating a range cell biological processes, very little is known about structure tissue culture cells. We focused on 5'-untranslated region human immunodeficiency virus type 1 RNA genome, highly conserved region, which contains structural domains that regulate key steps viral replication cycle. Up until now, information only came from vitro studies. Here, we developed chemical modification assays to test nucleotide accessibility directly infected...
The positive transcription elongation factor b (P-TEFb) is involved in physiological and pathological events including inflammation, cancer, AIDS, cardiac hypertrophy. balance between its active inactive form tightly controlled to ensure cellular integrity. We report that the transcriptional repressor CTIP2 a major modulator of P-TEFb activity. copurifies interacts with an complex containing 7SK snRNA HEXIM1. associates directly HEXIM1 and, via loop 2 snRNA, P-TEFb. In this nucleoprotein...
The retrovlral genome consists of two homologous RNA molecules associated close to their 5′ ends. We studied the spontaneous dimerization four HIV-1 fragments (RNAs 1–707, 1–615, 311–612, and 311–415) containing previously defined domain, a fragment (RNA 1–311) corresponding upstream sequences. Significant all RNAs Is observed on agarose gels when magnesium is Included In electrophoresls buffer. contrast 311–612 311–415, 1–615 1–311 strongly depends size monovalent cation present in...
The 5'-untranslated leader region of human immunodeficiency virus type 1 (HIV-1) RNA contains multiple signals that control distinct steps the viral replication cycle such as transcription, reverse genomic dimerization, splicing, and packaging. It is likely fine tuned coordinated regulation these functions achieved through specific RNA-protein RNA-RNA interactions. In a search for cis-acting elements important tertiary structure HIV-1 RNA, we identified, by ladder selection experiments,...
In addition to genomic RNA, HIV-1 particles package cellular and spliced viral RNAs. order determine the encapsidation mechanisms of these RNAs, we determined packaging efficiencies specificities singly fully HIV mRNAs different host RNAs species: 7SL U6 snRNA GAPDH mRNA using RT-QPCR. Except mRNA, all are selectively encapsidated. Singly harboring Rev-responsible element, which do not contain this motif, enriched in virions similar levels, even though they exported from nucleus by routes....
The HIV-1 viral infectivity factor (Vif) allows productive infection of non-permissive cells (including most natural targets) by counteracting the cellular cytosine deaminases APOBEC-3G (hA3G) and hA3F. Vif-induced degradation these restriction factors proteasome has been extensively studied, but little is known about translational repression hA3G hA3F Vif, which also proposed to participate in Vif function. Here, we studied binding mRNA its role repression. Filter assays fluorescence...