BackgroundDiabetic foot ulcers (DFUs) are a severe complication of diabetes. Persistent inflammation and impaired vascularization present considerable challenges in tissue wound healing. The aim this study was to identify the crucial regulators DFU healing investigate their specific mechanisms DFU.MethodsDFU RNA sequencing data were obtained feature genes. expression levels genes corresponding microRNAs (miRNAs) verified clinical samples. Subsequently, CD68 determined non-diabetic skin RAW...

Abstract Chronic atrophic gastritis (AG) is initiated mainly by Helicobacter pylori infection, which may progress to stomach cancer following the Correa's cascade. The current treatment regimen H. eradication, yet evidence lacking that this effective on later stages of AG especially gastric gland atrophy. Here, using mouse model, patient samples, organoids, and lineage tracing, study unraveled stem cell (GSC) defect as a crucial pathogenic factor in human. Moreover, natural peptide isolated...

Inflammation, initiated by polymorphonuclear neutrophil (PMNs) infiltration, is the first step in wound healing. The aim of this study to investigate function neutrophils a diabetes-impaired healing model and explore underlying mechanisms leading dysfunction. Superficial second-degree burns were created streptozotocin (STZ)-induced diabetic rat model, changes levels advanced glycation end products (AGE), receptor AGE (RAGE), inflammatory cytokines oxidative markers, as well cell apoptosis...

Dermatological problems in diabetes might play an important role the spontaneous ulcers and impaired wound healing that are seen diabetic patients. Investigation of cause skin disorders is critical for identifying effective treatment. The abdominal full-thickness tissues 33 patients (14 nondiabetic 19 diabetic) were analyzed. cell viability malondialdehyde (MDA) production fibroblasts measured after advanced glycosylation end product (AGE)-bovine serum albumin (BSA) exposure. Cutaneous...

Purpose: The study aimed to investigate whether astrocyte pyroptosis, and the subsequent neuroinflammatory response that exerts amyloid β (Aβ) neurotoxic effects, has an effect on endothelial cells, along with underlying mechanisms. Methods: In vivo, 5 μL of disease venom was injected into lateral ventricle APP/PS1 mice for treatment. Pyroptosis induced by treating astrocytes Aβ42 in vitro. Small interfering RNA (siRNA) used silence caspase-1 Gasdermin D (GSDMD) mRNA expression. Cell...

ABSTRACT Ferroptosis is an important factor affecting the progression of bladder cancer (BC). Previous studies have confirmed that discoidin domain receptor 1 (DDR1) promotes BC progression. However, regulatory mechanisms ferroptosis are largely unknown. Therefore, this study aimed to investigate effects DDR1 on cell ferroptosis. Ferroptosis‐sensitive and ‐resistant cells were screened, reverse‐transcription quantitative PCR western blotting used determine expression in cells. In vitro vivo...

BACKGROUND Stress ulcers are common complications in critically ill patients, with a higher incidence observed older patients following gastrointestinal surgery. This study aimed to develop and evaluate the effectiveness of multi-modal intervention protocol prevent stress this high-risk population. AIM To assess impact on preventing intensive care unit (ICU) postoperatively. METHODS A randomized controlled trial involving (aged ≥ 65 years) admitted ICU after surgery was conducted. Patients...

Complement factor I (CFI) deficiency is a rare primary immunodeficiency that disrupts the classical and alternative complement pathways, potentially causing severe recurrent infections autoimmune manifestations in pediatric patients. However, coexistence of both pathways patient extremely uncommon. We report seven-year-old with disorder who presented middle ear infections, paronychia, gastrointestinal respiratory infections. Genetic testing revealed previously unreported homozygous variant...

Abstract We aimed to explore the mechanism of circular RNAs (circRNAs) and provide potential biomarkers for molecular therapy diabetic foot ulcers (DFU). Gene expression profile GSE114248, including five normal samples DFU samples, was downloaded from GEO database. Differentially expressed circRNAs (DEcircRNAs) between two groups were identified. Then, DEcircRNA‐miRNA miRNA‐mRNA interaction revealed, followed by circRNA‐miRNA‐mRNA network construction. Moreover, functional pathway analysis...

This study investigated the gastroprotective effects and possible mechanism of Kangfuxin (KFX), an ethanol extract Periplaneta americana L. (Dictyoptera; Blattidae), on improving healing quality preventing recurrence gastric ulcer. The KFX were in patients treated with endoscopic submucosal dissection (ESD), ulcer animal model, rat mucosal cells fibroblasts. Moreover, relationship between p38/NF-κB pathway explored both vivo vitro. In patients, exhibited protective against ulcers resulted a...

To investigate the characteristics of diabetic patients with foot ulcers, their health-related quality life (HRQoL), and link between them. The study population included 131 consecutive presenting in a clinic new ulcer December 1, 2011, May 2012. authors collected sociodemographic data, using Wagner Grade, HRQoL (using SF-36 Scale) information; 54.2% were 2 or 3 categories. In all 8 subscales summary scales, had significantly poorer than general China ( P < .01). Their Grade negative...

To explore aminoguanidine (AG) effect on neutrophil functions and associated signal transduction way in diabetic rats wound healing. Sprague-Dawley (SD) were divided into 3 groups, Group A (control+burns), B (diabetes+burns), C (diabetes+burns+AG). Wound skin tissue was harvested at 6h, 24h 36h after trauma, then immunohistochemistry used to detect AGE (advanced glycation end products) contents RAGE (receptor of AGE) expression. Western blotting applied NF-κB. Oxidative stress changes...

Increased accumulation of advanced glycation end products (AGEs) in diabetic skin is closely related to delayed wound healing. Studies have shown that the concentration AGEs elevated tissues and not subcutaneous refractory wounds, which suggests there may be a causal relationship between two. In present study, vitro experiments revealed activated neutrophils, migratory adhesive functions neutrophils decreased once AGE levels reached certain threshold. Different expression differentially...

Abstract Negative pressure wound therapy (NPWT) has been widely used in various lesions. This study aimed to explore the biological effects of negative on polymorphonuclear neutrophils (PMNs), macrophages, and epidermal keratinocyte cells involved healing. PMNs differentiated from HL‐60, macrophages were derived THP‐1 monocytes, keratinocytes cultured vitro, they treated with 0, −0.03 mp, −0.05 respectively. Cell ultrastructure; viability; apoptosis; protein factors such as tumour necrosis...

The aim of the study was to explore effect aminoguanidine cream on skin tissue microenvironment in diabetic rats.A total 51 healthy male Sprague Dawley (SD) rats were randomly divided into three groups: diabetes group (n = 18), 18) and control 15). Rats injected with 65 mg/kg streptozotocin induce model, citrate buffer. After successful induction diabetes, back hair all stripped by barium sulfide, treated using disinfected cotton swabs twice every day for 40 days, while groups matrix....

To explore potential molecular mechanisms and novel biomarkers of mild cognitive impairment (MCI) Alzheimer's disease (AD).The mRNA expression datasets GSE63060 GSE63061 the miRNA dataset GSE120584 were obtained from Gene Expression Omnibus database. The differentially expressed genes (DEGs) (DEmiRs) identified in normal, MCI, AD groups. Mfuzz clustering weighted correlation network analyses (WGCNA) conducted, followed by pathway functional enrichment miRNA-mRNA construction. Furthermore,...

Methylglyoxal (MGO) is a highly reactive dicarbonyl compound formed during hyperglycaemia. MGO combines with proteins to form advanced glycation end products (AGEs), leading cellular dysfunction and organ damage. In type 2 diabetes mellitus (T2DM), the higher plasma concentration, lower extremity amputation rate. Here, we aimed identify mechanisms of MGO-induced dysfunction. We observed that accumulation MGO-derived AGEs in human diabetic wounds increased, whereas expression glyoxalase 1...

Background Diabetic peripheral neuropathy (DPN) is a serious complication in Diabetes Mellitus (DM) patients and the underlying mechanism yet unclear. Ferroptosis has been recently intensively researched as key process pathogenesis of diabetes but there no related bioinformatics-based studies context DPN Methods We used data mining analysis techniques to screen differentially expressed genes (DEGs) immune cell content with DPN, DM healthy participants (dataset GSE95849). These DEGs were then...

Mild cognitive impairment (MCI) is an intermediate state between normal aging and dementia characterized by pathological decline. The study aimed at revealing the risk of MCI progressing to through a follow-up investigation.In 2011, 441 subjects were recruited, disease status was tracked survey in 2017. Subjects with stable (MCIs; N = 356) progressed into (MCIp; 77) analysed our study. Community-dwelling old people age ≥ 55 recruited from 30 streets 24 committees (or communities) Pudong New...

To systematically review the diagnostic accuracy of spot urinary protein to creatinine ratio (PCR) and 12-h proteinuria in preeclampsia estimate which is a preferred alternative method for 24-h proteinuria, we carried out this meta-analysis. 25 primary studies were included based on searching strategy. For PCR, our results showed pooled sensitivity 87% (95% confidence interval [CI] 83%–91%) specificity 86% CI 79%–91%), with an area under curve (AUC) 0.93 (0.90–0.95). 92% 87%–96%) 99%...

Background: The pathological damage mechanism of type 2 diabetes (T2D) and macroangiopathy is extremely complex, T2D arteriosclerosis obliterans have different biological behaviors clinical features. To explore the lower extremity occlusion (LEAOD) in patients, we utilized RNA-seq to identify unique gene expression signatures LEAOD through transcriptomic analysis. Methods: We obtained blood samples performed RNA sequencing from four patients with T2D, five whom had LEAOD. Another six age-...

The correct thoughts and principles of diagnosis treatment chronic refractory wounds need to be formulated. Through the relevant domestic international consensus based on clinical experience, Thoughts in China is proposed. It considered that wounds, case fully understanding patient's medical history, following should complied order. (1) Pay attention cleanliness wound after being cleaned. (2) Reasonably perform debridement avoid " excessive" or not thorough". (3) examination, diagnosis,...

This study reports the case of a 71-year-old woman with type 2 diabetes whose paronychia rapidly progressed to right middle finger and then whole dorsal aspect necrosis hand. After admission, diagnosis diabetic hand ulcer was established Streptococcus agalactiae found as pathogen. The authors controlled glucose, used 3% hydrogen peroxide sulfadiazine silver in routine dressing, well surgical debridement, topical negative pressure, skin grafting. wound closed 32 days after surgery. Diabetic...