A5010447791- Polyomavirus and related diseases
- Plant Virus Research Studies
- Inflammasome and immune disorders
- Bacteriophages and microbial interactions
- Full-Duplex Wireless Communications
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Fungal and yeast genetics research
- Cellular transport and secretion
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- Parvovirus B19 Infection Studies
- Plant Disease Resistance and Genetics
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Phytoplasmas and Hemiptera pathogens
- RNA modifications and cancer
- Plant Pathogenic Bacteria Studies
- Insect and Pesticide Research
- Antenna Design and Analysis
- Immune Response and Inflammation
- RNA Research and Splicing
- vaccines and immunoinformatics approaches
Vilnius University
2012-2024
Nature Research Centre
2013
Abstract Distinct SARS-CoV-2 lineages, discovered through various genomic surveillance initiatives, have emerged during the pandemic following unprecedented reductions in worldwide human mobility. We here describe a lineage - designated B.1.620 Lithuania and carrying many mutations deletions spike protein shared with widespread variants of concern (VOCs), including E484K, S477N HV69Δ, Y144Δ, LLA241/243Δ. As well as documenting suite this carries, we also its potential to be resistant...
ABSTRACT Many high-income countries have met the SARS-CoV-2 pandemic with overwhelming sequencing resources and identified numerous distinct lineages, including some notably altered biology. Over a year into following unprecedented reductions in worldwide human mobility, introduced lineages of without sequenced antecedents are increasingly discovered as result ongoing genomic surveillance initiatives. We here describe one such lineage, carrying many mutations deletions spike protein shared...
Eleven new human polyomaviruses (HPyVs) have been identified in the last decade. Serological studies show that these novel HPyVs sub-clinically infect humans at an early age. The routes of infection, entry pathways, and cell tropism remain unknown. VP1 proteins can assembly into virus-like particles (VLPs). As culturing systems for HPyV are currently not available, VP1-derived VLPs may be useful tools basic research biotechnological applications.Recombinant from 11 newly were efficiently...
Heterologous gene expression is dependent on multistep processes involving regulation at the level of transcription, mRNA turnover, protein translation, and posttranslational modifications. Codon bias has a significant influence yields. However, sometimes it not clear which parameter causes observed differences in heterologous as codon adaptation typically optimizes many sequence properties once. In current study, we evaluated production human papillomavirus type 16 (HPV-16) major structural...
Abstract Data on the course of viral infections revealed severe inflammation as a consequence antiviral immune response. Despite extensive research, there are insufficient data role innate cells in promoting mediated by complexes (IC) antigens and their specific antibodies. Recently, we demonstrated that human polyomaviruses (PyVs) induce an inflammatory response macrophages. Here, investigated macrophage activation IC. We used primary murine macrophages cell model, virus-like particles...
Protein engineering provides an opportunity to generate new immunogens with desired features. Previously, we have demonstrated that hamster polyomavirus major capsid protein VP1-derived virus-like particles (VLPs) are highly immunogenic and can be employed for the insertion of foreign epitopes at certain surface-exposed positions. In current study, designed pseudotype VLPs consisting intact VP1 VP2 fused target antigen—cellular marker p16 INK4A —at its N terminus. Both proteins coexpressed...
Polyomaviruses (PyVs) are highly prevalent in humans and animals. PyVs cause mild illness, however, they can also elicit severe diseases. Some potentially zoonotic, such as simian virus 40 (SV40). However, data still lacking about their biology, infectivity, host interaction with different PyVs. We investigated the immunogenic properties of virus-like particles (VLPs) derived from viral protein 1 (VP1) human immunised mice recombinant HPyV VP1 VLPs mimicking structure viruses compared...
Viral antigens can activate phagocytes, inducing inflammation, but the mechanisms are barely explored. The aim of this study is to investigate how viral oligomeric proteins different structures induce inflammatory response in macrophages. Human THP-1 cell line was used prepare macrophages that were treated with filamentous nucleocapsid-like particles (NLPs) paramyxoviruses and spherical virus-like (VLPs) human polyomaviruses. effects on viability, pro-inflammatory cytokines' production,...
Although there are similarities in the core steps of secretion pathway from yeast to higher eukaryotes, significant functional differences exist even among diverse species. Here, we used next-generation sequencing identify two mutations Kluyveromyces lactis KlSEC59 gene, encoding dolichol kinase (DK), which responsible for an enhanced phenotype a previously isolated mutant, MD2/1-9. Compared with temperature-sensitive Saccharomyces cerevisiae sec59-1 exhibits reduced N-glycosylation and...
Protein glycosylation is a fundamental modification crucial for numerous intra- and extracellular functions in all eukaryotes. The phosphorylated dolichol (Dol-P) utilized N-linked protein other pathways. Dolichol kinase (DK) plays key role catalyzing the phosphorylation of dolichol. patterns Kluyveromyces lactis DK mutant revealed that yeast well tolerated minor deficiency Dol-P by adjusting glycosylation. Comparative analysis sequences homologs from different species eukaryotes, archaea...
Abstract Viral antigens can activate phagocytes inducing inflammation but the mechanisms are barely explored. This study aimed to investigate capability of viral oligomeric proteins different structure induce inflammatory response in macrophages. Human THP-1 cell line was used prepare macrophages which were treated with filamentous nucleocapsid-like particles (NLPs) paramyxoviruses and spherical virus-like (VLPs) human polyomaviruses. The effects on viability, pro-inflammatory cytokines’...