A5082975435- Prostate Cancer Treatment and Research
- Cancer, Hypoxia, and Metabolism
- Bone health and treatments
- Cancer Cells and Metastasis
- TGF-β signaling in diseases
- Cancer, Lipids, and Metabolism
- Radiopharmaceutical Chemistry and Applications
- Cancer Research and Treatments
- Cell Adhesion Molecules Research
- Medical Imaging Techniques and Applications
- Bladder and Urothelial Cancer Treatments
- Wnt/β-catenin signaling in development and cancer
- Nanoplatforms for cancer theranostics
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer-related gene regulation
- Medical Imaging and Pathology Studies
- Bone Metabolism and Diseases
- Virus-based gene therapy research
- Bone and Dental Protein Studies
- Viral gastroenteritis research and epidemiology
- Steroid Chemistry and Biochemistry
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Connective Tissue Growth Factor Research
- Adipose Tissue and Metabolism
Leiden University Medical Center
2013-2025
Leiden University
2007-2021
Loyola University Medical Center
2011
University of Bern
2010
University of Oxford
2010
University of Sheffield
2010
Neurology, Inc
2007
During bone formation and fracture healing there is a cross-talk between endothelial cells osteoblasts. We previously showed that vascular growth factor A (VEGF-A) might be an important in this cross-talk, as osteoblast-like produce angiogenic differentiation-dependent manner. Moreover, exogenously added VEGF-A enhances osteoblast differentiation. In the present study we investigated, given coupling angiogenesis formation, whether morphogenetic proteins (BMPs) stimulate osteoblastogenesis...
Metastatic progression of advanced prostate cancer is a major clinical problem. Identifying the cell(s) origin in and its distant metastases may permit development more effective treatment preventive therapies. In this study, aldehyde dehydrogenase (ALDH) activity was used as basis to isolate compare subpopulations primary human cells cell lines. ALDH-high displayed strongly elevated clonogenicity migratory behavior vitro. More strikingly, readily formed with enhanced tumor at both...
Sclerostin is an osteocyte-derived negative regulator of bone formation. It inhibits BMP-stimulated formation both in vitro and vivo but has no direct effect on BMP signaling. Instead, sclerostin Wnt signaling that required for osteoblastic differentiation.Sclerostin a member the Dan family glycoproteins which many members have been reported to antagonize activity. shown inhibit formation, its mechanism action seems be different from classical antagonists. In this study, we investigated by...
Abstract Bone morphogenetic protein 7 (BMP7) counteracts the physiological epithelial-to-mesenchymal transition (EMT), a process that is indicative of epithelial plasticity. Because EMT involved in cancer, we investigated whether BMP7 plays role breast cancer growth and metastasis. In this study, show decreased expression primary significantly associated with formation clinically overt bone metastases patients ≥10 years follow-up. line these clinical observations, inversely related to...
Abstract We examined the role of Wnt/β-catenin signaling in successive stages osteoblast differentiation. It has been shown that Wnt mature osteoblasts needs to be downregulated enable formation a mineralized matrix. Using RNA interference, we showed this is, at least part, accomplished by upregulation antagonists Dickkopf-1 and -2. Introduction: The initiation differentiation well studied. However, during late-stage is less clear. have Materials Methods: treated murine bone marrow...
Acquisition of an invasive phenotype by cancer cells is a requirement for bone metastasis. Transformed epithelial can switch to motile, mesenchymal epithelial-mesenchymal transition (EMT). Recently, it has been shown that EMT functionally linked prostate stem cells, which are not only critically involved in maintenance but also We showed treatment with the non-peptide αv-integrin antagonist GLPG0187 dose-dependently increased E-cadherin/vimentin ratio, rendering more epithelial, sessile...
High aldehyde dehydrogenase (ALDH) activity can be used to identify tumor-initiating and metastasis-initiating cells in various human carcinomas, including prostate cancer. To date, the functional importance of ALDH enzymes carcinogenesis, progression metastasis has remained elusive. Previously we identified strong expression ALDH7A1 cancer cell lines, primary tumors matched bone metastases. In this study, evaluated whether is required for acquisition a metastatic stem/progenitor phenotype...
Background/Aims Brown adipose tissue (BAT) dissipates energy stored in triglycerides as heat via the uncoupling protein UCP-1 and is a promising target to combat hyperlipidemia obesity. BAT densely innervated by sympathetic nervous system, which increases differentiation activity upon cold exposure. Recently, Bone Morphogenetic Protein 7 (BMP7) was identified an inducer of differentiation. We aimed elucidate role activation effect BMP7 on treating mice with at varying ambient temperature,...
Abstract Altered microRNA (miRNA; miR) expression is associated with tumor formation and progression of various solid cancers. A major challenge in miRNA profiling bulk tumors represented by the heterogeneity subpopulations cells that constitute organ, as well tissue. Here, we analyzed miRNAs a subpopulation epithelial stem/progenitor-like human prostate cancer [prostate stem cell (PCSC)] compared their profile to more differentiated cells. In both lines clinical specimens, identified miR-25...
Improved targeted therapies are needed to combat metastatic prostate cancer. Here, we report the identification of spleen kinase SYK as a mediator dissemination in zebrafish and mouse xenograft models human Although has not been implicated previously this disease, found that its expression is upregulated cancers associated with malignant progression. RNAi-mediated silencing prevented invasive outgrowth vitro bone colonization vivo, effects were reversed by wild-type but kinase-dead...
Resistance to docetaxel is a major clinical problem in advanced prostate cancer (PCa). Although glucocorticoids (GCs) are frequently used combination with docetaxel, it unclear what extent GCs and their receptor, the glucocorticoid receptor (GR), contribute chemotherapy resistance. In this study, we aim elucidate role of GR docetaxel-resistant PCa order improve current therapies. expression was analyzed tissue microarray primary specimens from chemonaive docetaxel-treated patients, cultured...
Background The inflammatory tumor microenvironment, and more specifically the tumor‐associated macrophages, plays an essential role in development progression of prostate cancer towards metastatic bone disease. Tumors are often characterized by a leaky vasculature, which ‐ combined with prolonged circulation kinetics liposomes leads to efficient localization these drug carriers, via so‐called enhanced permeability retention (EPR) ‐effect. In this study, we evaluated utility targeted,...
Urological malignancies, including prostate and bladder carcinoma, represent a major clinical problem due to the frequent occurrence of therapy resistance formation incurable distant metastases. As result, there is an urgent need for versatile predictive disease models assessment individualized drug response in urological malignancies. Compound testing on ex vivo cultured patient-derived tumor tissues could promising approach. In this study, we have optimized culture system explanted human...
The transforming growth factor (TGF)-β superfamily comprises cytokines such as TGF-β and Bone Morphogenetic Proteins (BMPs), which have a critical role in multitude of biological processes. In breast cancer, high levels are associated with poor outcome, whereas inhibition TGF-β-signaling reduces metastasis. contrast, BMP-7 inhibits bone metastasis cancer cells.In this study, we investigated the effect on TGF-β-induced invasion 3 dimensional assay.BMP-7 inhibited metastatic cell line...
Abstract Background Tumor cell migration and metastasis require dynamic rearrangements of the actin cytoskeleton. Interestingly, F-actin cross-linking stabilizing protein L-plastin, originally described as a leukocyte specific protein, is aberrantly expressed in several non-hematopoietic malignant tumors. Therefore, it has been discussed tumor marker. However, systematic vivo analyses functional relevance L-plastin for were so far lacking. Methods We investigated expression phosphorylation...