A5012617221- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Neuroendocrine regulation and behavior
- Stress Responses and Cortisol
- Neurogenetic and Muscular Disorders Research
- Ion channel regulation and function
- Photoreceptor and optogenetics research
- Amyotrophic Lateral Sclerosis Research
- Zebrafish Biomedical Research Applications
- Neural dynamics and brain function
- Receptor Mechanisms and Signaling
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neural Engineering
- Parkinson's Disease Mechanisms and Treatments
- Adipose Tissue and Metabolism
- Congenital heart defects research
- Epilepsy research and treatment
- Neurogenesis and neuroplasticity mechanisms
- Historical and Linguistic Studies
- Neurobiology and Insect Physiology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA modifications and cancer
- Muscle activation and electromyography studies
- Mitochondrial Function and Pathology
- Retinal Development and Disorders
Columbia University
2017-2025
Instituto de Neurociencias
2021-2024
Universitat de Miguel Hernández d'Elx
2023-2024
Consejo Superior de Investigaciones Científicas
2023-2024
Columbia University Irving Medical Center
2017-2024
University of Valparaíso
2018
Université Paris Cité
2009-2016
Centre de Neurophysique Physiologie et Pathologie
2009-2016
Centre National pour la Recherche Scientifique et Technique (CNRST)
2016
Centre National de la Recherche Scientifique
2013-2016
Recent results suggest that social memory requires the dorsal hippocampal CA2 region as well a subset of ventral CA1 neurons. However, it is unclear whether and represent parallel or sequential circuits. Moreover, because evidence implicating in comes largely from long-term inactivation experiments, dynamic role remains unclear. Here, we use pharmacogenetics optogenetics mice to acutely reversibly silence its projections hippocampus. We show activity critical for encoding, consolidation,...
The hippocampus is essential for different forms of declarative memory, including social the ability to recognize and remember a conspecific. Although recent studies identify importance dorsal CA2 region in memory storage, little known about its sources information. Because CA2, like other hippocampal regions, receives major source spatial non-spatial information from medial lateral subdivisions entorhinal cortex (MEC LEC), respectively, we investigated these inputs memory. Whereas MEC are...
In amyotrophic lateral sclerosis (ALS) the large motoneurons that innervate fast-contracting muscle fibers (F-type motoneurons) are vulnerable and degenerate in adulthood. contrast, small slow-contracting (S-type resistant do not degenerate. Intrinsic hyperexcitability of F-type during early postnatal development has long been hypothesized to contribute neural degeneration adult. Here, we performed a critical test this hypothesis by recording from identified F- S-type superoxide dismutase-1...
The fast contraction time of mouse motor units creates a unique situation in which motoneurons have to fire at low frequencies produce small forces but also very high frequency (much higher than cat or rat motoneurons) reach the fusion their units. To understand how this problem is solved, we performed intracellular recordings adult spinal and investigated systematically subthreshold properties discharge pattern. We show that much wider range firing because three salient features. First,...
Spinal muscular atrophy (SMA), a recessive neurodegenerative disease, is characterized by the selective loss of spinal motor neurons. No available therapy exists for SMA, which represents one leading genetic causes death in childhood. SMA caused mutation survival-of-motor-neuron 1 ( SMN1 ) gene, to quantitative defect survival-motor-neuron (SMN) protein expression. All patients retain or more copies SMN 2 modulates disease severity producing small amount stable protein. We reported recently...
Alterations in thalamic dopamine (DA) or DA D2 receptors (D2Rs) have been measured drug addiction and schizophrenia, but the relevance of D2Rs for behavior is largely unknown. Using situ hybridization mice expressing green fluorescent protein (GFP) under Drd2 promoter, we found that D2R expression within thalamus enriched paraventricular nucleus (PVT) as well more ventral midline nuclei. Within PVT, are inhibitory their activation inhibits neuronal action potentials brain slices....
Neuronal activity modulates the membrane diffusion of postsynaptic γ-aminobutyric acid (GABA)(A) receptors (GABA(A)Rs), thereby regulating efficacy GABAergic synapses. The K289M mutation in GABA(A)Rs subunit γ2 has been associated with generalized epilepsy febrile seizures plus (GEFS+) syndrome. This accelerates receptor deactivation and therefore reduces inhibitory synaptic transmission. Yet, it is not clear why this specifically promotes seizures. We show that upon raising temperature both...
The dorsal and ventral regions of the CA1 field hippocampus play distinct roles in encoding cognitive vs. emotional behaviors, respectively. Whether this distinction applies to other hippocampal fields behaviors is unclear. Here, we focus on CA2 compare properties behavioral its (dCA2) (vCA2) regions. Although dCA2 known be required for social memory promote aggression, role vCA2 unknown. We report that a defined region extends extreme pole hippocampus, with certain distinctions dCA2. Unlike...
Amyotrophic lateral sclerosis (ALS) is a lethal disorder characterized by the gradual degeneration of brainstem and spinal motoneurons as well cortico-spinal tracts. The onset generally occurs during adult age except for some juvenile aggressive forms. Until recently, vast majority cases (90%) were deemed sporadic. Mutations in SOD1 gene have been long time only ones reported familiar forms ALS. However, recent implication new genes little known function cast view on this disease. C9ORF72,...
During cerebral cortex development, excitatory pyramidal neurons (PNs) establish specific projection patterns while receiving inputs from GABAergic inhibitory interneurons (INs). Whether these can shape PNs’ is, however, unknown. While layer 4 (L4) PNs of the primary somatosensory (S1) are all born as long-range callosal (CPNs), most them acquire local connectivity upon activity-dependent elimination their interhemispheric axons during postnatal development. Here, we demonstrate that precise...
De novo mutations in voltage- and ligand-gated channels have been associated with an increasing number of cases developmental epileptic encephalopathies, which often fail to respond classic antiseizure medications. Here, we examine two knock-in mouse models replicating de sequence variations the human HCN1 voltage-gated channel gene, p.G391D p.M153I (Hcn1G380D/+ Hcn1M142I/+ mouse), severe drug-resistant neonatal- childhood-onset epilepsy, respectively. Heterozygous mice from both lines...
In neonatal mice, fast- and slow-type motoneurons display different patterns of discharge. response to a long liminal current pulse, the discharge is delayed up several seconds in fast-type their firing frequency accelerates. contrast, immediately, decreases at beginning pulse. Here, we identify ionic currents that underlie motoneurons. We find delay caused by combination an A-like potassium transiently suppresses on short time scale slowly-inactivating inhibits over much longer scale. then...
Hyperpolarization-activated, cyclic nucleotide–gated (HCN) channels generate the cationic I h current in neurons and regulate excitability of neuronal networks. The function HCN depends, part, on their subcellular localization. Of four isoforms (HCN1-4), HCN1 is strongly expressed dendrites pyramidal (PNs) hippocampal area CA1 but also presynaptic terminals parvalbumin-positive interneurons (PV+ INs), which provide strong inhibitory control over activity. Yet, little known about how these...
A17 amacrine cells are an important part of the scotopic pathway. Their synaptic varicosities receive glutamatergic inputs from rod bipolar (RBC) and release GABA onto same RBC terminal, forming a reciprocal feedback that shapes depolarization. Here, using patch-clamp recordings, we characterized electrical coupling between rat retina report presence strongly interconnected non-coupled cells. In coupled cells, evoked currents preferentially flow out cell through GJs cross-synchronization...