A5024781595- Receptor Mechanisms and Signaling
- Memory and Neural Mechanisms
- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Nicotinic Acetylcholine Receptors Study
- Optical Coherence Tomography Applications
- Radiomics and Machine Learning in Medical Imaging
- Advanced Fluorescence Microscopy Techniques
- Neural dynamics and brain function
- Photoacoustic and Ultrasonic Imaging
- Genetics and Neurodevelopmental Disorders
- Mitochondrial Function and Pathology
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Immunotherapy and Biomarkers
- Metabolism and Genetic Disorders
- Medical Imaging Techniques and Applications
- Cannabis and Cannabinoid Research
- Endometrial and Cervical Cancer Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
Agilent Technologies (United States)
2023-2024
Columbia University
2017-2022
New York Psychoanalytic Society and Institute
2020-2021
New York State Psychiatric Institute
2020-2021
University of California, San Diego
2017-2018
Glutamate is the dominant excitatory neurotransmitter in brain, but under conditions of metabolic stress it can accumulate to excitotoxic levels. Although pharmacologic modulation amino acid receptors well studied, minimal consideration has been given targeting mitochondrial glutamate metabolism control Here we demonstrate that chemical inhibition pyruvate carrier (MPC) protects primary cortical neurons from death. Reductions uptake do not compromise cellular energy metabolism, suggesting...
Experience-dependent expression of immediate-early gene transcription factors (IEG-TFs) can transiently change the transcriptome active neurons and initiate persistent changes in cellular function. However, impact IEG-TFs on circuit connectivity function is poorly understood. We investigate specificity with which IEG-TF NPAS4 governs experience-dependent inhibitory synaptic input onto CA1 pyramidal (PNs). show that novel sensory experience selectively enhances somatic inhibition mediated by...
Alterations in thalamic dopamine (DA) or DA D2 receptors (D2Rs) have been measured drug addiction and schizophrenia, but the relevance of D2Rs for behavior is largely unknown. Using situ hybridization mice expressing green fluorescent protein (GFP) under Drd2 promoter, we found that D2R expression within thalamus enriched paraventricular nucleus (PVT) as well more ventral midline nuclei. Within PVT, are inhibitory their activation inhibits neuronal action potentials brain slices....
In the striatum, acetylcholine (ACh) neuron activity is modulated co-incident with dopamine (DA) release in response to unpredicted rewards and reward-predicting cues both neuromodulators are thought regulate each other. While this co-regulation has been studied using stimulation studies, existence of mutual regulation vivo during natural behavior still largely unexplored. One long-standing controversy whether striatal DA responsible for induction cholinergic pause or D2 receptors (D2Rs)...
Abstract Ultra-compact micro-optical elements for endoscopic instruments and miniaturized microscopes allow non-invasive non-destructive examination of microstructures tissues. With sub-cellular level resolution such could provide immediate diagnosis that is virtually consistent with a histologic enabling example to differentiate the boundaries between malignant benign tissue. Such are now being developed at rapid rate; however, current manufacturing technologies limit very large sizes, well...
Ultra-compact miniaturized optical components for microendoscopic tools and microscopes are required minimally invasive imaging. Current technologies used deep tissue imaging procedures limited to a large diameter and/or low resolution due manufacturing restrictions. We demonstrate platform miniaturization of an system applications with 1 µm. designed our probe using cascaded micro-lenses waveguides (lensguide) achieve as small 100 µm x field view 60 in diameter. wide-field microscopy based...
In the United States, PD-L1 IHC 22C3 pharmDx indications for use have expanded to include assay’s as an aid in identifying triple-negative breast cancer (TNBC) patients treatment with pembrolizumab (KEYTRUDA®). Analytical validation TNBC was conducted by Agilent Technologies support of device performance and FDA-approval. studies included precision (inter-instrument, inter-operator, inter-day, inter-lot, intra-run) external reproducibility (inter- intra-site, inter- intra-observer) using...
Abstract Cholinergic interneurons (CINs) in the striatum respond to salient stimuli with a multiphasic response, including pause, neuronal activity. Slice physiology experiments have shown importance of dopamine D2 receptors (D2Rs) regulating CIN pausing yet behavioral significance pause and its regulation by vivo is still unclear. Here, we show that D2R upregulation CINs nucleus accumbens (NAc) lengthens activity ex enlarges stimulus-evoked decrease acetylcholine (ACh) levels during...
Abstract Background: PD-L1 IHC 22C3 pharmDx (SK006) is a qualitative immunohistochemical (IHC) assay using anti-PD-L1 clone to detect in formalin-fixed, paraffin-embedded (FFPE) human endometrial cancer (ENDO) tissues. expression determined the Combined Positive Score (CPS) algorithm, which number of staining cells (tumor cells, lymphocytes, macrophages) divided by total viable tumor multiplied 100. Validation testing evaluated sensitivity and analytical performance SK006 FFPE ENDO specimens...
Abstract Ventral striatal dopamine is thought to be important for associative learning. Dopamine exerts its role via activation of D1 and D2 receptors in the ventral striatum. Upregulation D2R indirect pathway nucleus accumbens (NAc) impairs incentive motivation inhibiting synaptic transmission pallidum. Here, we determined whether upregulation D2Rs resulting impairment function modulates learning an auditory Pavlovian reward task as well Go/No-Go operant based driven task. We found that...
Ventral striatal dopamine is thought to be important for associative learning. Dopamine exerts its role via activation of D1 and D2 receptors in the ventral striatum. Upregulation D2R striatopallidal neurons impairs incentive motivation inhibiting synaptic transmission pallidum. Here, we determined whether upregulation D2Rs resulting impairment pathway function modulates learning an auditory Pavlovian reward task as well Go/No-Go operant based driven task. We found that did not affect or...
Abstract In the striatum, acetylcholine (ACh) neuron activity is modulated co-incident with dopamine (DA) release in response to unpredicted rewards and reward predicting cues both neuromodulators are thought regulate each other. While this co-regulation has been studied using stimulation studies, existence of mutual regulation vivo during natural behavior still largely unexplored. One long-standing controversy whether striatal DA responsible for induction cholinergic pause or D2R modulate a...