A5028084685- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Inflammasome and immune disorders
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Neonatal Respiratory Health Research
- Rabies epidemiology and control
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cell death mechanisms and regulation
- Viral Infections and Immunology Research
- Ferroptosis and cancer prognosis
- Immune Response and Inflammation
- Bacillus and Francisella bacterial research
- Viral Infections and Outbreaks Research
- Insect Resistance and Genetics
- Cytomegalovirus and herpesvirus research
- Zoonotic diseases and public health
- Respiratory viral infections research
Hospital Israelita Albert Einstein
2018-2024
Instituto de Ensino e Pesquisa Santa Casa
2024
World Health Organization Regional Office for the Americas
2023
Brigham and Women's Hospital
2020-2022
Harvard University
2020-2022
Abstract Prime editing enables search-and-replace genome but is limited by low efficiency. We present a high-throughput approach, the Peptide Self-Editing sequencing assay (PepSEq), to measure how fusion of 12,000 85-amino acid peptides influences prime show that peptide can enhance editing, prime-enhancing combine productively, and top dual peptide-prime editor increases significantly in multiple cell lines across dozens target sites. Top function increasing translation efficiency serve as...
Respiratory syncytial virus (RSV) is the major cause of acute bronchiolitis in infants under 2 years old. Necroptosis has been implicated outcomes respiratory infections. We report that RSV infection triggers necroptosis primary mouse macrophages and human monocytes a RIPK1-, RIPK3- MLKL-dependent manner. Moreover, pathways are harmful to clearance from alveolar macrophages. Additionally, Ripk3 −/− mice were protected RSV-induced weight loss presented with reduced viral loads lungs. Alveolar...
The adenosine analogue remdesivir has emerged as a frontline antiviral treatment for SARS-CoV-2, with preliminary evidence that it reduces the duration and severity of illness 1 . Prior clinical studies have identified adverse events 1,2 , been shown to inhibit mitochondrial RNA polymerase in biochemical experiments 7 yet little is known about specific genetic pathways involved cellular metabolism cytotoxicity. Through genome-wide CRISPR-Cas9 screening sequencing, we show leads repression...
Abstract Prime editing enables search-and-replace genome but is limited by low efficiency. We present a high-throughput approach, PepSEq, to measure how fusion of 12,000 85-amino acid peptides derived from human DNA repair-related proteins influences prime show that peptide can enhance editing, prime-enhancing combine productively, and top dual peptide-prime editor increases significantly in multiple cell lines across dozens target sites.