Hanseniaspora vineae is an apiculate yeast that plays a significant role at the beginning of fermentation, and it has been studied for its application in improvement aromatic profile commercial wines. This work evaluates use H. alcoholic fermentation compared to Saccharomyces cerevisiae ageing on lees process (AOL) non-Saccharomyces yeasts. The results indicated there were not differences basic oenological parameters. completed until 11.9% v/v ethanol with residual sugars content less than 2...

Abstract Collagen hydrolysates are dietary supplements used for nutritional and medical purposes. They complex mixtures of low‐molecular‐weight peptides obtained from the enzymatic hydrolysis collagen, which provide intrinsic batch‐to‐batch heterogeneity. In consequence, quality these products, is related to reproducibility their mass distribution pattern, should be addressed. Here, we propose an analytical approach determine peptide pattern as a attribute Colagenart®, product containing...

Dichondra argentea is a plant used in traditional medicine, where it has been torelieve stomach pain, regulate fever and treat heart conditions, among other uses.Therefore, essential to support its use with scientific studies that validate itseffectiveness safety the medicinal field. In present work, total contentof phenols flavonoids was determined by spectrometric analysis, antioxidantactivity evaluated measuring blocking capacity of free radicals 2,2-diphenyl-1-picrylhydrazyl (DPPH *)...

The analytical potential of multinuclear NMR using chiral liquid crystals to discriminate between enantiomers axially biaryls is explored.

Novel band selective decoupled pure shift refocusing experiments allowed simplification of the measurement all δ¹H, and <italic>J</italic>_HH couplings with an ultrahigh spectral resolution in peptides.

Abstract Annona purpurea has been traditionally used by indigenous and socioeconomically disadvantaged people to treat infectious parasitic diseases, including amoebiasis. The goal of this study was assess the effect a crude methanolic extract, an alkaloid aporphine alkaloids from leaves A. on viability Entamoeba histolytica trophozoite cultures identify mechanism action. Different concentrations extracts purpureine (1), 3-hydroxyglaucine (2), norpurpureine (3) glaziovine (4), oxopurpureine...

Hanseniaspora vineae and opuntiae are apiculate yeasts normally found on the skins of ripe grapes at beginning alcoholic fermentation. Several studies have reported that these species can provide interesting sensory characteristics to wine by contributing high levels acetate esters increase mouthfeel body wines. The present work aims evaluate use two sequentially with Saccharomyces cerevisiae improve profile Albillo Mayor white fermentations were carried out in triplicate 150 L stainless...

The quality of foods has led researchers to use various analytical methods determine the amounts principal food constituents; some them are NMR techniques with a multivariate statistical analysis (NMR-MSA). present work introduces set NMR-MSA novelties. First, double pulsed-field-gradient echo (DPFGE) experiment refocusing band-selective uniform response pure-phase selective pulse for excitation 5–10-ppm range wine samples reveals novel broad 1H resonances. Second, an foodomics approach...

Identity is a critical quality attribute that must be determined before releasing batches of medicinal and dietary products. However, the identities peptide-derived products composed large number diverse molecules challenging since most analytical techniques cannot analyze multiple simultaneously. Here, we proposed determination weight-average molecular weight (Mw) polydispersity index (PDI) by mass spectrometry for control batch release complex products, namely, glatiramer acetate...

The oenological industry has benefited from the use of Nuclear Magnetic Resonance (1H-NMR) spectroscopy in combination with Multivariate Statistical Analysis (MSA) as a foodomics tool for retrieving discriminant features related to geographical origins, grape varieties, and further quality controls. Said omics methods have gained such attention that Intergovernmental Organizations Control Agencies are currently recommending their massive amongst countries compliances tracking standard...

Elucidation of the coordination modes in pyrimidylmethylamines by NMR and DFT methods.

Overlapping 13C or 15N solid-state NMR spectra from crystallographically different forms of l-arginine hydrochloride can be separated by exploiting differential proton T1 relaxation in conjunction with cross-polarization. Dipolar 13C–13C and 15N–15N two-dimensional correlation experiments reveal resonances belonging to magnetically inequivalent molecules.

Abstract The presence of water has been shown to deeply impact the stability and geometry Zn complexes in solution. Evidence for tetra‐ penta‐coordinated species a pyridylmethylamine–Zn II model complex is presented. Novel 1 H NMR tools such as T ‐filtered selective exchange spectroscopy pure shifted gradient‐encoded refocusing well classical 2D ( H– H) spectroscopy, diffusion‐ordered measurements, combination with density functional theory methods allow full conformational dynamics be...

In agreement with the draft resolution OENO-SCMA 17-618 at step 5 “ Quantitation of glucose, malic acid, acetic fumaric shikimic acid and sorbic in wine using proton nuclear magnetic resonance spectroscopy ( 1 H NMR)” said technique has been recently accepted within OIV chair as a primary quantitative analytical for beverage analysis such wine. However, poor chemical shift dispersion NMR spectra severely penalizes quantification overlapped or crowded regions. To outflank penalization...

Insight into structural and motional features of the C-terminal part Human Centrin 2 in complex with peptide P17-XPC was obtained by using complementary solid-state NMR methods. We demonstrate that experimental conditions procedures sample crystallization determine quality spectra internal mobility protein. Two-dimensional (2D) 13C–13C 15N–15N correlation reveal intra- inter-residue dipolar connectivities provide partial, site-specific assignments 13C 15N resonance signals. The secondary...

Present work comprises the use of different multivariate spectroscopic methods for tracking novel metabolomics signatures related to red wine chemistry. It is presented first time proton nuclear magnetic resonance fingerprint a monovarietal Mexican Merlot, obtained with acquisition improvements recently proposed OIV Methods Analysis sub-commission. Effective multi-presaturation solvent schemes have revealed rich (poly)-phenolics aromatic region, so far not exploited wine-fingerprinting or –...

The deterioration of the skin accentuates over time, affecting its aesthetic appearance. This is characterized by weakening mechanisms involved in regeneration and repair dermal matrix. Consequently, losses elasticity smoothness resulting formation wrinkles. alternatives for facial rejuvenation include surgery, injection botulinum toxin, application masks. Topic products are less invasive, can be self-applied, have an increased benefit/risk relationship.We developed a liquid formulation...

Abstract Zea mays L corps apport to human consumption, complex matrices of compounds such as free and bounded phenolics, flavonoids anthocyanins with high nutritional values proved health benefits, which are dynamically synthesized since sprouting grains. This study presents for the first time a Nuclear Magnetic Resonance (NMR) non-targeted metabolomics aqueous methanolic extracts Mexican native blue red L. sprouts, produced specific germination scheme, at three different controlled...

The optimum conditions for the formation of plate-like and urchin-like microcrystals biomolecules their transfer to rotors solid-state NMR spectroscopy depend on a variety factors, which minimizing manipulation storing sample several months at 277 K (4 °C) play an important role. Three biological systems were investigated: Hen Egg-White (HEW) lysozyme (129 residues), lengthened C-terminal domain (LCter) Human centrin 2 (89 complex between (Cter) (79 residues) P17-XPC peptide (17 residues).