A5030279651- Advanced NMR Techniques and Applications
- NMR spectroscopy and applications
- Molecular spectroscopy and chirality
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Hemophilia Treatment and Research
- Advanced MRI Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Electron Spin Resonance Studies
- Lanthanide and Transition Metal Complexes
- Synthesis and Characterization of Heterocyclic Compounds
- Solid-state spectroscopy and crystallography
- X-ray Diffraction in Crystallography
- Synthesis and biological activity
- Amino Acid Enzymes and Metabolism
- Magnetism in coordination complexes
- Cancer, Hypoxia, and Metabolism
- Adipose Tissue and Metabolism
- Spectroscopy and Quantum Chemical Studies
- Synthesis and Reactions of Organic Compounds
- Crystallization and Solubility Studies
- Advanced Glycation End Products research
- Economic and Financial Impacts of Cancer
- Adolescent and Pediatric Healthcare
- Metabolism, Diabetes, and Cancer
Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques
2020-2025
Université Paris Cité
2019-2025
Sorbonne Paris Cité
2019-2025
Centre National de la Recherche Scientifique
2013-2025
Association Française de Chirurgie
2022-2025
Inserm
2024-2025
Association Francaise contre les Myopathies
2023-2024
Biomedtech Facilities
2024
Délégation Paris 5
2019-2024
Centre Hospitalier Universitaire de Besançon
2023
Ultrahigh-resolution pure shift NMR has recently been shown as a promising approach for providing quantitative metabolic profiles that can be used to study the footprint left by cancer cells in their aqueous growth medium. In this approach, library of reference 1H spectra with water suppression was implemented determine metabolite concentrations from NOESY-presat-PSYCHE-SAPPHIRE spectrum recorded on extracellular This achievement clearly called generalization quantification method relying...
A detailed analysis of nitrogen-15 longitudinal relaxation times in microcrystalline proteins is presented. theoretical model to quantitatively interpret developed terms motional amplitude and characteristic time scale. Different averaging schemes are examined order propose an curves that takes into account the specificity MAS experiments. In particular, it shown magic angle spinning averages rate experienced by a single spin over one rotor period, resulting individual dependent on...
It is shown how coherence lifetimes in solid-state NMR experiments can be controlled. New decoupling schemes are introduced which actively optimize dephasing times, providing increases of up to a factor 2 with respect the best existing schemes. The new implemented transverse-dephasing-optimized (TDOP) for disorded solid cellulose, and microcrystalline protein, where sensitivity improvements 5 obtained.
Finger on the pulse: Selective experiments can be simultaneously run different parts of sample when NMR sequences based a spatial encoding are used. This approach was applied to gradient-encoded selective refocusing sequence. The resulting data provide collection all couplings that involve given proton spin, which conveniently assigned and measured from only one spectrum. Detailed facts importance specialist readers published as "Supporting Information". Such documents peer-reviewed, but not...
A new correlation experiment cited as "push-G-SERF" is reported. In the resulting phased 2D spectrum, chemical shift information selected along direct dimension, whereas scalar couplings involving a proton nucleus are edited in indirect domain. The robustness of this pulse sequence demonstrated on compounds with increasing structural and spectral complexity, using state-of-the-art spectrometers. It allows for full resolution both dimensions yielding straightforward assignment measurement...
Site-specific nitrogen-15 longitudinal relaxation rates are measured for the microcrystalline dimeric form of protein Crh using multidimensional high-resolution solid-state NMR methods. The used to provide a qualitative description site-specific internal mobility present in solid state.
We present the application of a 2D broadband homodecoupled proton NMR experiment to visualization enantiomers. In chiral environment, existence diastereoisomeric intermolecular interactions can yield-generally slight-variations chemical shifts from one enantiomer another. show that this approach, which relies on spatial encoding sample, is particularly well suited analysis enantiomeric mixtures, since it allows, within single experiment, detect subtle shift differences between enantiomers,...
Crystal clear: Strong supramolecular interactions between tris(dipicolinate)lanthanide complexes and arginine-like moieties have been evidenced by NMR chemical shift titration X-ray diffraction analyses. Since the lanthanide atom is a strong anomalous scatterer, complex itself luminescent, this interaction could be used as powerful tool in preparation of crystals protein derivatives with high phasing power. Supporting information for article available on WWW under...
In humans, genetic defects of the synthesis or regeneration tetrahydrobiopterin (BH4), an essential cofactor in hydroxylation reactions, are associated with severe neurological disorders. The diagnosis these conditions relies on determination BH4, dihydrobiopterin (BH2), and dihydroneopterin (NH2) cerebrospinal fluid (CSF). As MS/MS is less sensitive than fluorescence detection (FD) for this purpose, most widely used method since 1980 involves two HPLC runs including differential off-line...
Background/Objectives: Diffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin lymphoma, characterized by aggressive and heterogeneous tumors originating from B-cells. Especially in patients with relapsed or refractory (R/R) disease, DLBCL remains a challenging cancer to treat. Metabolic reprogramming hallmark malignant cells. Our research focuses on developing strategies enhance clinical outcomes for R/R targeting metabolic vulnerabilities. Methods: We investigated...
ABSTRACT Introduction There are few data on healthcare resource use and related costs of French haemophilia A (HA) B (HB) patients. Aims This study aimed to describe the profile HA HB patients, current disease management, clinical burden costs. Methods Data patients all ages alive 1/1/2022 were extracted from nationwide claims database (SNDS). Patients divided into four treatment groups: on‐demand or prophylaxis with without inhibitors. Haemophilia compared a control group (ratio 1:3)...
Abstract Background Methylglyoxal (MGO) is a potent glycating agent that contributes to the pathogenesis of diabetes. However, MGO unstable in plasma without demanding sample preparation at blood collection, limiting its clinical utility as biomarker. We aimed discover reliable MGO-glycated albumin (ALB)-derived biomarkers and assess their association with new-onset diabetes (NOD) people prediabetes. Methods Bottom-up mass spectrometry-based proteomics was used peptide ALB, including...
Abstract Diffusion ordered NMR is implemented to determine accurately the mobility of paramagnetic tris‐dipicolinate lanthanide complexes that are versatile probes protein structure. It shown diffusion coefficient ratios can be measured with an accuracy 1 % using a standard BPPLED pulse sequence, which allows for observing significant, though weak, variations when different species interacting compound. We demonstrate this approach complementary classical chemical shift titration...
Novel band selective decoupled pure shift refocusing experiments allowed simplification of the measurement all δ<sup>1</sup>H, and <italic>J</italic><sub>HH</sub> couplings with an ultrahigh spectral resolution in peptides.
Ultrahigh-resolution NMR has recently attracted considerable attention in the field of complex samples analysis. Indeed, implementation broadband homonuclear decoupling techniques allowed us to greatly simplify crowded 1H spectra, yielding singlets for almost every proton site from analyzed molecules. Pure shift methods have notably shown be particularly suitable deciphering mixtures metabolites biological samples. Here, we successfully implemented a new pure pulse sequence based on PSYCHE...
The observation of proton to nitrogen-15 heteronuclear Overhauser effects in the microcrystalline protein Crh is used confirm that principal mechanism relaxation amide nitrogens due fluctuation N-H dipolar couplings caused by bond dynamics. Our observations reveal central role water as main source magnetization, and we provide an analysis different pathways could lead observed results.