A5090648189- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- Cardiovascular Disease and Adiposity
- Immune Cell Function and Interaction
- Atherosclerosis and Cardiovascular Diseases
- Connective tissue disorders research
- Galectins and Cancer Biology
- Mobile Health and mHealth Applications
- Telemedicine and Telehealth Implementation
- Sex and Gender in Healthcare
- Lymphatic System and Diseases
- RNA modifications and cancer
- Lipid metabolism and disorders
- Dietary Effects on Health
- Reproductive System and Pregnancy
- Metabolism, Diabetes, and Cancer
- Fibroblast Growth Factor Research
- TGF-β signaling in diseases
- Immune cells in cancer
- RNA Research and Splicing
- Wnt/β-catenin signaling in development and cancer
- IL-33, ST2, and ILC Pathways
- Mental Health and Patient Involvement
- RNA and protein synthesis mechanisms
University of Michigan
2011-2020
Association on Higher Education And Disability
2016
Michigan United
2016
Ann Arbor Center for Independent Living
2015
Objective The relationship between adipose tissue fibrosis, adipocyte hypertrophy, and preadipocyte hyperplasia in the context of obesity correlation these tissue‐based phenomena with systemic metabolic disease are poorly defined. goal this study was to clarify human determine adipose‐tissue based diabetes. Methods Visceral subcutaneous tissues from humans collected during bariatric surgery were studied QRTPCR, immunohistochemistry, flow cytometry for expression collagens fibrosis‐related...
Age-related adiposity has been linked to chronic inflammatory diseases in late life. To date, the studies on adipose tissue leukocytes and aging have not taken into account heterogeneity of macrophages (ATMs), nor they examined how age impacts other such as T cells fat. Therefore, we performed a detailed examination ATM subtypes young old mice using state art techniques. Our results demonstrate qualitative changes ATMs with that generate decrease resident type 2 (M2) ATMs. The profile fat...
Obesity is associated with an activated macrophage phenotype in multiple tissues that contributes to tissue inflammation and metabolic disease. To evaluate the mechanisms by which obesity potentiates myeloid activation, we evaluated hypothesis activates cell production from bone marrow progenitors potentiate inflammatory responses tissues. High fat diet-induced generated both quantitative increases as well a potentiation of macrophages derived these progenitors. In vivo, hematopoietic stem...
The proinflammatory activation of leukocytes in adipose tissue contributes to metabolic disease. How crosstalk between immune cells initiates and sustains inflammation remains an unresolved question. We have examined the hypothesis that macrophages (ATMs) interact with regulate function T cells. Dietary obesity was shown activate proliferation effector memory CD4(+) tissue. Our studies further demonstrate ATMs are functional antigen-presenting promote interferon-γ-producing from lean obese...
An adaptive immune response triggered by obesity is characterized the activation of adipose tissue CD4(+) T cells unclear mechanisms. We have examined whether interactions between macrophages (ATMs) and contribute to metainflammation. Intravital microscopy identifies dynamic antigen-dependent ATMs in visceral fat. Mice deficient major histocompatibility complex class II (MHC II) showed protection from diet-induced obesity. Deletion MHC expression led an tissue-specific decrease...
Obesity causes dramatic proinflammatory changes in the adipose tissue immune environment, but relatively little is known regarding how this inflammation responds to weight loss (WL). To understand mechanisms by which meta-inflammation resolves during WL, we examined leukocytes mice after withdrawal of a high-fat diet. After 8 weeks achieved similar weights and glucose tolerance values as age-matched lean controls showed abnormal insulin tolerance. Despite fat mass normalization, total CD11c+...
Women of reproductive age are protected from metabolic disease relative to postmenopausal women and men. Most preclinical rodent studies skewed toward the use male mice study obesity-induced dysfunction because a similar protection observed in female mice. How sex differences inflammatory responses contribute these observations is unknown. We have compared contrasted effects high fat diet-induced obesity on glucose metabolism leukocyte activation multiple depots C57Bl/6 With both short term...
Thrombospondin 1 (THBS1 or TSP-1) is a circulating glycoprotein highly expressed in hypertrophic visceral adipose tissues of humans and mice. High-fat diet (HFD) feeding induces the robust increase THBS1 early stages HFD challenge. The loss Thbs1 protects male mice from diet-induced weight gain adipocyte hypertrophy. Hyperinsulinemic euglycemic clamp study has demonstrated that Thbs1-null are protected HFD-induced insulin resistance. Tissue-specific glucose uptake revealed insulin-sensitive...
Abstract Obesity-related changes in adipose tissue leukocytes, particular macrophages (ATMs) and dendritic cells (ATDCs), are implicated metabolic inflammation, insulin resistance, altered regulation of adipocyte function. We evaluated stromal cell white (WAT) expansion dynamics with high fat diet (HFD) feeding for 3–56 days, quantifying ATMs, ATDCs, endothelial (ECs), preadipocytes (PAs) visceral epididymal WAT subcutaneous inguinal WAT. To better understand mechanisms the early response to...
The extracellular matrix (ECM) plays important roles in maintaining adequate adipose tissue function and metabolic regulation. Here we have examined the organization of a relatively unexplored ECM component, elastin its response to diet induced obesity mice. Additionally, explored regulation requirement macrophage metalloelastase, MMP-12, remodeling obesity. In visceral fat depots, fibers form mesh-like net that becomes denser with diet-induced contrast, subcutaneous depots are more linear...
Objective Weight regain after weight loss is common, and there evidence to suggest negative effects on health because of cycling. This study sought investigate the impact in formerly obese mice adipose tissue architecture stromal cell function. Methods A diet‐switch model was employed for obesity induction, loss, mice. Flow cytometry quantified leukocytes tissue. Liver depots were compared determine tissue‐specific Results Epididymal white failed expand response repeat exposure high‐fat diet...
Abstract Obesity induces inflammation in adipose tissue involving the recruitment and activation of macrophages, CD8+ CD4+ T cells. These cells play a critical role development insulin resistance seen with obesity. Weight loss can improve health, but little is known concerning effects weight on immune activity tissue. Diet switch from 60% high-fat diet to 13% normal-fat was used induce diet-induced obese C57Bl/6 mice. Adipose fibrosis macrophage accumulation evaluated by histology. Leukocyte...
Partners Connected Health develops and validates technology-based solutions aimed at facilitating collaborative care, self-management, improved quality. From ideation to implementation, our team envisions builds innovations that facilitate Current often do not meet their goals of promoting self-care due limited integration into the clinical workflow, low sustainability patient, provider, caregiver engagement. The core mission is define future technology-enabled care delivery by integrating...
Abstract Obesity causes dramatic changes to the adipose tissue immune environment including recruitment and activation of macrophages. The inflammatory polarization these cells is thought play a critical role in severity insulin resistance associated with obesity. Weight loss can improve resistance, but little known concerning effects weight on activity adipose. Diet switch from 60% high-fat diet 13% normal-fat was used induce diet-induced obese C57Bl/6 mice. Adipose structural were...