Photobiomodulation (PBM) is rapidly gaining traction as a valuable tool in dermatology for treating many inflammatory skin conditions using low levels of visible light or near-infrared radiation. However, the physiological regulatory pathways responsible anti-inflammatory effect PBM have not been well defined. Since previous studies showed that nuclear factor-erythroid 2 like (Nrf2) master regulator response, we addressed its role controlling inflammation by PBM. Primary human keratinocytes...

The influence of injectable hyaluronic gels on skin's microbiota is unclear. As well, skin a key factor modulating final effect gels. ex-vivo study was aimed at alterations following acid injection into the dermis in non-sterile surface conditions. Ex vivo human explants presence or absence either S. epidermidis aureus, were treated with control excipient (0.9% sodium chloride) test product (Hyaluronic S, HA-S). Bacterial analysis performed, as well structural integrity. Histological imaging...

Synopsis Polyphenols in general are compounds that known to promote health and have a preventive effect against various chronic diseases. The influence of cocoa polyphenols on skin, however, has scarcely been studied from histological point view. aim this study is assess the several indicators skin elasticity tonus, namely, glycosaminoglycans collagen I, III IV. This was carried out by using model ex vivo human explants maintained survival, which polyphenol extract applied. After processing...

Hydrofluoric acid (HF) is particularly dangerous due to the potential for systemic effects and induction of severe skin necrosis through two mechanisms: corrosiveness local tissue toxicity. In addition, because it only partially dissociated (pK(a) 3.2), capable penetrating deeply into tissues. There a lack experimental studies that objectively characterize behavior HF diffusion human skin, specifically kinetics penetration resulting in cellular lesions.We describe cutaneous using an...

<b><i>Background:</i></b> Accumulation of advanced glycation end-products (AGEs) in skin has been associated with aging. Inhibition proteins extracellular matrix may help texture and appearance. The objective the study was to demonstrate antiglycation activity topically applied carnosine novel facial cream (FC) containing human explants ex vivo. <b><i>Methods:</i></b> Glycation induced by methylglyoxal (MG) culture media. FC (FC-CARN) or...

Background: Hydrofluoric acid (HF) is a small and partially dissociated (pKa 3.2), able to deeply penetrate into human skin in addition the corrosiveness of hydrogen ion (H+) toxicity fluoride (F−). However, there has been lack experimental studies objectively characterize results HF exposure decontamination.Methodology/principal findings: A previously established method using explants ex vivo model (Part 1. Experimental 70% hydrofluoric burns: Histological observations an model) described...

Synopsis Glycation is an ageing reaction of naturally occurring sugars with dermal proteins, whose clinical signs may appear in vivo around age 30, and increases steadily/regularly age. The suppleness the dermis affected by formation bridges between proteins (Maillard’s reaction). residues formed (Amadori products, Advanced End products) as well they alter, can be visualized specific immunostainings. Induced a few days on living skin explants methylglyoxal, glycation prevented application...

The aim of the paper presented herein is description and safety evaluation process dissolution an 86-microneedle patch composed hyaluronic acid, when applied topically to human abdominal skin explants. Such explants were chosen replace inability obtaining periorbital skin. In order evaluate penetration microneedles, we employed histochemical methods a fluorescent dye FITC (fluorescein isothiocyanate).Abdominoplasty treated with square microneedle patches 1.5-cm2 surface area, containing 86...

Skin is being increasingly exposed to artificial blue light due the extensive use of electronic devices, which can induce cell oxidative stress, causing signs early photo aging. The Melissa officinalis phytocomplex a new standardized cosmetic ingredient obtained by an in vitro plant culture with high content rosmarinic acid. In this study, we examine activity protect skin against and infrared damages, evaluating ROS (Radical Oxygen Species) level keratinocyte line from human (HaCaT) Nrf2...

Fitchia nutans (astéracée), plante d'un genre endémique de Polynésie française, autrefois utilisée traditionnellement pour les soins la peau comme ingrédient des monoï sacrés, fait partie cosmétopée polynésienne. Les extraits feuille F. ont été soumis à tests d'activité anti-âge, sur explants ex vivo montrant leurs potentialités stimuler croissance collagène et d'élastine dermique. L'étude phytochimique cette plante, réalisée première fois, montre que constituants principaux sont...

Synopsis In a normal and healthy skin, the regular elimination of superficial corneocytes, called desquamation, is fundamental physiologic process intended to protect barrier function skin. This invisible loss individually or in small groups, incessantly compensated by divisions proliferative layer upward cellular maturation order maintain harmonious renewal epidermis integrity stratum corneum. The harmony this desquamation intimately conditioned sufficient hydration corneum: (i) an abnormal...

Existing methods to evaluate skin care products suffer limitations. This is the case for ex vivo explants, a first-choice 3D model. While essential analyse mid- long-term biological effects, this classical model hinders assessing microrelief variations. To circumvent these limitations, we developed an PERFused EXplant setup (Perfex) that maintains outer surface in open air, closely mirroring physiological conditions.

Fitchia nutans Hook.f. (Asteraceae), a Polynesian endemic plant locally called "'anei", was previously used as skin care ingredient included in sacred traditional monoï preparation French Polynesia [1]. Such use indicated interesting properties to investigate. For that purpose, leaves extract of F. tested on ex-vivo human for anti-ageing activity assays systems could mirror the morphological and immunophenotypic tissues [2]. Abdominal this study were collected from surgical procedures....

The skin is a formidable physical and biological barrier which communicates continuously with the outside of body. And stratum corneum, outermost layer human epidermis, plays central role in interaction between cutaneous tissue external environment. horny layer, more generally whole layers, avoid penetration harmful exogenous agents, produce molecules named anti-microbial peptides impact composition microbiota, regulate internal corporal temperature, water loss from inside body constitute an...

Hydrofluoric acid (HF) induces severe skin necrosis through corrosive damage and tissue toxicity. As it is only partially dissociated (pKa 3.2), the small HF molecule penetrates deeply into tissues. When absorbed circulatory system, can dissociate H+ F- ions (the latter binds calcium magnesium releases potassium from erythrocytes), which together with resultant metabolic acidosis may result in cardiovascular system toxicity dysrhythmias death. A summary review of recent experimental data...