A5102903430- Drug Transport and Resistance Mechanisms
- Cholesterol and Lipid Metabolism
- Lipid Membrane Structure and Behavior
- Pneumonia and Respiratory Infections
- Pediatric Hepatobiliary Diseases and Treatments
- Amino Acid Enzymes and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Peptidase Inhibition and Analysis
- ATP Synthase and ATPases Research
- Trace Elements in Health
- Streptococcal Infections and Treatments
- Metabolism and Genetic Disorders
- Enzyme Structure and Function
- High Entropy Alloys Studies
- Antibiotic Resistance in Bacteria
- Transcranial Magnetic Stimulation Studies
- Photosynthetic Processes and Mechanisms
- Glycosylation and Glycoproteins Research
- RNA modifications and cancer
- Anesthesia and Neurotoxicity Research
- Plant nutrient uptake and metabolism
- Particle physics theoretical and experimental studies
- Histone Deacetylase Inhibitors Research
- Digestive system and related health
- Phytase and its Applications
University of Science and Technology of China
2013-2025
Zhejiang University of Technology
2024
Hebei University of Technology
2022-2023
Hefei National Center for Physical Sciences at Nanoscale
2007-2022
Chinese Academy of Sciences
2021-2022
Nanjing Tech University
2016
Nankai University
2013
Bilirubin is mainly generated from the breakdown of heme when red blood cells reach end their lifespan. Accumulation bilirubin in human body usually leads to various disorders, including jaundice and liver disease. conjugated hepatocytes excreted bile duct via ATP-binding cassette transporter ABCC2, dysfunction which would lead Dubin-Johnson syndrome. Here we determine structures ABCC2 apo, substrate-bound ATP/ADP-bound forms using cryo-electron microscopy, exhibiting a full with regulatory...
Abstract A facile visible‐light‐assisted solution‐phase approach has been successfully developed to synthesize trigonal Te 1D nanostructures. By varying the relative amount of H 2 TeO 3 and water‐soluble polymers, wirelike, beltlike, tubular Te, nanoparticle‐joined aggregates, as well a novel thorny assembly nanothreads can be synthesized on large scale. The diameter nanowires modulated by controlling nucleation growth process through modulation pH value reaction mixture. It is believed that...
Previous studies have found that the novel low-elastic-modulus Ti2448 alloy can significantly reduce stress shielding and contribute to better bone repair than conventional Ti6Al4V alloy. In this study, promotion of osteogenesis angiogenesis by three-dimensionally printed were also observed in vivo. However, these not significant a series vitro tests. The stiffness materials has been reported greatly affect response macrophages, immunological regulation mediated macrophages directly...
SignificanceATP8B1 is a P4 ATPase that maintains membrane asymmetry by transporting phospholipids across the cell membrane. Disturbance of lipid will lead to imbalance and eventually, death. Thus, defects in ATP8B1 are usually associated with severe human diseases, such as intrahepatic cholestasis. The present structures complexed its auxiliary noncatalytic partners CDC50A CDC50B reveal an autoinhibited state could be released upon substrate binding. Moreover, release this autoinhibition...
The wall teichoic acid (WTA) is a major cell component of Gram-positive bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), common cause fatal clinical infections in humans. Thus, the indispensable ABC transporter TarGH, which flips WTA from cytoplasm to extracellular space, becomes promising target anti-MRSA drugs. Here, we report 3.9-Å cryo-electron microscopy (cryo-EM) structure 50% sequence-identical homolog TarGH Alicyclobacillus herbarius at an ATP-free and...
The reverse cholesterol transport pathway is responsible for the maintenance of human homeostasis, an imbalance which usually leads to atherosclerosis. As a key component this pathway, ATP-binding cassette transporter ABCG1 forwards cellular extracellular acceptor nascent high-density lipoprotein (HDL). Here, we report 3.26-Å cryo-electron microscopy structure cholesterol-bound in inward-facing conformation, represents turnover condition upon ATP binding. Structural analyses combined with...
Human ABC transporter ABCD1 transports very long-chain fatty acids from cytosol to peroxisome for β-oxidation, dysfunction of which usually causes the X-linked adrenoleukodystrophy (X-ALD). Here, we report three cryogenic electron microscopy structures ABCD1: apo-form, substrate- and ATP-bound forms. Distinct what was seen in previously reported transporters, two symmetric molecules behenoyl coenzyme A (C22:0-CoA) cooperatively bind transmembrane domains (TMDs). For each C22:0-CoA,...
The spr0693-spr0694-spr0695 operon of Streptococcus pneumoniae encodes a putative ATP-binding cassette (ABC)-type efflux pump involved in the resistance antibiotics and antimicrobial peptides. Here we report crystal structures Spr0694-0695 at 3.3 Å Spr0693 3.0 resolution, revealing MacAB-like pump. dimeric adopts non-canonical fold ABC transporter, transmembrane domain which consists eight tightly packed helices with an insertion extracellular between first second helices, whereas forms...
Abstract The human ABC transporter ABCC3 (also known as MRP3) transports a wide spectrum of substrates, including endogenous metabolites and exogenous drugs. Accordingly, it participates in multiple physiological processes is involved diverse diseases such intrahepatic cholestasis pregnancy, which caused by the intracellular accumulation bile acids estrogens. Here, we report three cryogenic electron microscopy structures ABCC3: apo‐form complexed forms bound to either conjugated sex hormones...
Coordinated carbon and nitrogen metabolism is crucial for bacteria living in the fluctuating environments. Intracellular homeostasis maintained by a sophisticated network, which widespread signaling protein PII acts as major regulatory hub. In cyanobacteria, was proposed to regulate nitrate uptake an ABC (ATP-binding cassette)-type transporter NrtABCD, nucleotide-binding domain of NrtC fused with C-terminal (CRD). Here, we solved three cryoelectron microscopy structures NrtBCD, bound...
Abstract Life on Earth relies carbon and nitrogen assimilation by RubisCO GS-GOGAT enzymes, respectively, whose activities depend a constant supply of inorganic (C i ) (N). Members the PII signal transduction superfamily are among most ancient widespread cell signaling proteins in nature. One their highly conserved functions is controlling C - N-transporters, feature found different phyla Archaea both Gram-positive Gram-negative bacteria. Recently, we identified PII-like protein SbtB as...
The homeostasis of intracellular diadenosine 5′,5″′-P1,P4-tetraphosphate (Ap4A) in the yeast Saccharomyces cerevisiae is maintained by two 60% sequence-identical paralogs Ap4A phosphorylases (Apa1 and Apa2). Enzymatic assays show that, compared to Apa1, Apa2 has a relatively higher phosphorylase activity towards Ap3A (5′,5″′-P1,P3-tetraphosphate), Ap4A, Ap5A (5′,5″′-P1,P5-tetraphosphate), favorable substrate for both enzymes. To decipher catalytic insights, we determined crystal structures...
Abstract Two new 3D organic‐inorganic hybrid materials Cu(bim)V 2 O 6 ( 1 ) (bim=bis(1‐imidazolyl)methane) and Co(2‐mbim)V (2‐mbim=bis(2‐methylimidazol‐1‐yl)methane) based on polyoxovanadates (POVs) organic ligands decorated transition metal units have been synthesized by solvothermal methods. Single crystal XRD, soild UV‐vis IR spectra, powder magnetic measurement thermogravimetric (TG) measurements were performed to analyze the structures properties of . The topological both compounds are...