A5027210076- Helicobacter pylori-related gastroenterology studies
- Antifungal resistance and susceptibility
- Galectins and Cancer Biology
- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Eosinophilic Esophagitis
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Microbial Metabolites in Food Biotechnology
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Neonatal and Maternal Infections
- Veterinary medicine and infectious diseases
- Tryptophan and brain disorders
- Gut microbiota and health
- Pneumonia and Respiratory Infections
- Probiotics and Fermented Foods
- Influenza Virus Research Studies
- Fungal Infections and Studies
- vaccines and immunoinformatics approaches
- Bacterial Infections and Vaccines
- Complement system in diseases
- Neuropeptides and Animal Physiology
- Ear Surgery and Otitis Media
- Bacterial Identification and Susceptibility Testing
The Kids Research Institute Australia
2022-2025
The University of Western Australia
2003-2024
Charité - Universitätsmedizin Berlin
2024
Humboldt-Universität zu Berlin
2024
Freie Universität Berlin
2024
Queen Elizabeth II Medical Centre
2009-2016
Pathwest Laboratory Medicine
2016
World Health Organization - Pakistan
2001-2004
World Health Organization
2003
University of Geneva
2003
Despite the availability of efficacious drugs, success treating hypertension is limited by patients' inconsistent drug intake. Immunization against angiotensin II may offer a valuable alternative to conventional drugs for treatment hypertension, because vaccines induce relatively long-lasting effects and do not require daily dosing. Here we describe preclinical development phase I clinical trial testing virus-like particle (VLP)-based antihypertensive vaccine.An II-derived peptide was...
The in-vivo plasma concentration of penicillin needed to prevent Streptococcus pyogenes pharyngitis, recurrent acute rheumatic fever, and progressive heart disease is not known. We used a human challenge model assess the minimum required streptococcal pharyngitis. In CHIPS, randomised, double-blind, placebo-controlled, trial, healthy adult volunteers were randomly assigned by computer-generated random sequence target steady-state concentrations (placebo, 3, 6, 9, 12, or 20 ng/mL). study was...
Background According to the WHO, more than 1 billion people worldwide are overweight and at risk of developing chronic illnesses, including cardiovascular disease, type 2 diabetes, hypertension stroke. Current therapies show limited efficacy often associated with unpleasant side-effect profiles, hence there is a medical need for new therapeutic interventions in field obesity. Gastric inhibitory peptide (GIP, also known as glucose-dependent insulinotropic polypeptide) has recently been...
Helicobacter pylori infection causes chronic active gastritis that after many years of can develop into peptic ulceration or gastric adenocarcinoma. The bacterium is highly adapted to surviving in the environment and a key adaptation virulence factor urease. Although widely postulated, requirement urease expression for persistent has not been elucidated experimentally as conventional knockout mutants are incapable colonization. To overcome this constraint, conditional H. were constructed by...
Abstract The factors limiting neonatal and infant IgG Ab responses to T-dependent Ags are only partly known. In this study, we assess how these B cell influenced by the postnatal development of spleen lymph node microarchitecture. When BALB/c mice were immunized with alum-adsorbed tetanus toxoid at various stages their immune development, a major functional maturation step for induction serum IgG, Ab-secreting cells, germinal center (GC) was identified between second third week life. This...
Infections caused by the yeast Candida albicans represent an increasing threat to debilitated and immunosuppressed patients, neutropenia is important risk factor. Monoclonal antibody depletion of neutrophils in mice was used study role these cells host resistance. Ablation increased susceptibility both systemic vaginal challenge. The fungal burden kidney threefold on day 1, 100-fold 4, infection associated with extensive tissue destruction. However, a striking feature disseminated disease...
Early life antibody responses are characterized by a rapid decline, such that antigen-specific IgG antibodies decline to baseline levels within months following infant immunization. This generic observation remains unexplained. Here, we have analyzed the induction and organ-localization of antibody-secreting cells (ASC) immunization 1-week-old or adult BALB/c mice with tetanus toxoid (TT), T-dependent antigen. priming induced only slightly lower numbers TT-specific ASC in spleen, these...
The enzymatic activity of Helicobacter pylori's urease neutralises stomach acidity, thereby promoting infection by this pathogen. Urease protein has also been found to interact with host cells in vitro, although property's possible functional importance not studied vivo. To test for a role the surface host/pathogen interaction, exposed loops that display high thermal mobility were targeted inframe insertion mutagenesis. H. pylori expressing insertions at four eight sites tested retained...
Helicobacter pylori lipopolysaccharide promotes chronic gastric colonisation through O-antigen host mimicry and resistance to mucosal antimicrobial peptides mediated primarily by modifications of the lipid A. The structural organisation core domains H. remains unclear, as attachment site has still be identified experimentally. Here, investigations lipopolysaccharides purified from two wild-type strains ligase mutant revealed that core-oligosaccharide domain is a short conserved...
Helicobacter pylori genomes typically contain 8 or 9 genes that code for secreted and highly disulfide‐bridged proteins designated cysteine‐rich (Hcp). Here we show HcpA (hp0211) but not HcpC (hp1098) triggers the differentiation of human myeloid Thp1 monocytes into macrophages. Small amounts cause transition round‐shaped cells with star‐like morphologies, adherence to culture dish surface, phagocytosis opsonized fluorescent microspheres, expression surface marker protein CD11b, all which...
Streptococcus pyogenes (Strep A) infections result in a vastly underestimated burden of acute and chronic disease globally. The Strep A Vaccine Global Consortium's (SAVAC's) mission is to accelerate the development safe, effective, affordable S. vaccines. safety vaccine recipients paramount importance. single clinical trial conducted 1960s raised important concerns. SAVAC Safety Working Group was established review assessment methodology results more recent early-phase trials consider future...
After systemic infection with the yeast Candida albicans, inbred mice show substantial differences in mortality, organ colonization, and severity of tissue damage. To examine relationships between these variables, which are not directly correlated each other, fungal colonization kidneys brain was enumerated six strains that exhibit different patterns damage mortality. Mice lacking fifth component complement (C5) highly susceptible to lethal challenge, A/J DBA/2 mice, both C5 deficient,...
ABSTRACT Although benzylpenicillin (penicillin G) is listed by the World Health Organization as an Essential Medicine, dose optimization a persistent challenge, especially for long-acting intramuscular formulations. Maintaining sustained antibiotic exposure at target concentrations crucial secondary chemoprophylaxis of rheumatic heart disease and treatment syphilis. This study compared pharmacokinetic profile continuous low-dose infusions with standard-dose bolus evaluated which renal...
One mechanism utilized by bacterial pathogens for host adaptation and immune evasion is the generation of phenotypic diversity phasevarion that results from differential expression a suite genes regulated activity phase-variable methyltransferase within restriction modification (RM) system. Phasevarions are active in Helicobacter pylori, however there have been no studies investigating significance RM systems on colonization.Two mutant types incapable phase variation were constructed; clean...
In the model organism E. coli, recombination mediated by related XerC and XerD recombinases complexed with FtsK translocase at specialized dif sites, resolves dimeric chromosomes into free monomers to allow efficient chromosome segregation cell division. Computational genome analysis of Helicobacter pylori, a slow growing gastric pathogen, identified just one chromosomal xer gene (xerH) its cognate site (difH). Here we show that between directly repeated difH sites requires XerH, but not...
Abstract Background Despite vaccination, influenza and otitis media (OM) remain leading causes of illness. We previously found that the human respiratory commensal Haemophilus haemolyticus prevents bacterial infection in vitro related murine Muribacter muris delays OM development mice. The observation M pretreatment reduced lung titer inflammation suggests these bacteria could be exploited for protection against influenza/OM. Methods Safety efficacy intranasal H at 5 × 107 colony-forming...
Abstract Background A future Streptococcus pyogenes (Strep A) vaccine will ideally prevent a significant burden of lower limb cellulitis; however, natural immune responses to proposed antigens following an episode cellulitis remain uncharacterized. Methods We enrolled 63 patients with and 26 invasive beta hemolytic streptococci infection, using multiplexed assay measure immunoglobulin G against Strep candidate antigens, including: streptolysin O (SLO), deoxyribonuclease B (DNB), group...
Pharyngitis, more commonly known as sore throat, is caused by viral and/or bacterial infections. Group A Streptococcus (Strep A) the most common cause of pharyngitis. Strep pharyngitis an acute, self-limiting disease but if undertreated can lead to suppurative complications, nonsuppurative poststreptococcal immune-mediated diseases, and toxigenic presentations. We present a standardized surveillance protocol, including case definitions for pharyngitis, well classifications that be used...
The role of T lymphocytes in host responses to sublethal systemic infection with Candida albicans was evaluated by mAb depletion CD4+ and CD8+ cells from BALB/c CBA/CaH mice, which develop mild severe tissue damage, respectively. Depletion mice markedly increased but did not alter the course infection. In abrogated destruction both brain kidney at day 4 after infection, significantly decreased fungal colonization brain. However, severity lesions relative controls 8 onwards. A small increase...