A5053396495- Inflammatory mediators and NSAID effects
- DNA Repair Mechanisms
- Advanced Neuroimaging Techniques and Applications
- Cardiac electrophysiology and arrhythmias
- Mass Spectrometry Techniques and Applications
- Receptor Mechanisms and Signaling
- Adipose Tissue and Metabolism
- Estrogen and related hormone effects
- Advanced MRI Techniques and Applications
- Carcinogens and Genotoxicity Assessment
- Ion channel regulation and function
- Ophthalmology and Visual Impairment Studies
- Nuclear Structure and Function
- Epigenetics and DNA Methylation
- Agriculture, Soil, Plant Science
- Retinal Imaging and Analysis
- CRISPR and Genetic Engineering
- DNA and Nucleic Acid Chemistry
- Dietary Effects on Health
- Advanced biosensing and bioanalysis techniques
- Click Chemistry and Applications
- Anesthesia and Neurotoxicity Research
- Horticultural and Viticultural Research
- Gut microbiota and health
- Corneal surgery and disorders
Xuzhou Medical College
2025
Second People’s Hospital of Huai’an
2025
First Hospital of Shanxi Medical University
2021-2025
Shanxi Medical University
2021-2025
First Hospital of China Medical University
2024
University of Electronic Science and Technology of China
2024
First People's Hospital of Foshan
2007-2024
China Medical University
2024
Tianjin First Center Hospital
2024
Shandong First Medical University
2024
A sensitive regulator of cellular potassium class channels called K2P modulates resting membrane potential in most cells. The are regulated by multiple ligands, including the antidepressant drug Prozac, as well factors such mechanical stretch and voltage. Dong et al. determined structure human channel, TREK-2, two conformations bound to a metabolite Prozac. structures show how ligand binding or might induce switching between states. Although both states have open channels, one appears primed...
Mutations in the nuclear membrane zinc metalloprotease ZMPSTE24 lead to diseases of lamin processing (laminopathies), such as premature aging disease progeria and metabolic disorders. processes prelamin A, a component lamina intermediate filaments, by cleaving it at two sites. Failure this results accumulation farnesylated, membrane-associated A. The 3.4 angstrom crystal structure human has seven transmembrane α-helical barrel structure, surrounding large, water-filled, intramembrane...
We present Hunyuan3D 2.0, an advanced large-scale 3D synthesis system for generating high-resolution textured assets. This includes two foundation components: a shape generation model -- Hunyuan3D-DiT, and texture Hunyuan3D-Paint. The generative model, built on scalable flow-based diffusion transformer, aims to create geometry that properly aligns with given condition image, laying solid downstream applications. benefiting from strong geometric priors, produces vibrant maps either generated...
Cyclooxygenase-2 (COX-2) overexpression is prominent in inflammatory diseases, neurodegenerative disorders, and cancer. Directly monitoring COX-2 activity within its native environment poses an exciting approach to account for illuminate the effect of local environments on protein activity. Herein, we report development CoxFluor, first activity-based sensing live cells with confocal microscopy flow cytometry. CoxFluor strategically links a natural substrate dye precursor engage both...
Fusarium head blight (FHB), one of the most important diseases wheat, mainly occurs in ear. Given that severity disease cannot be accurately identified, cost pesticide application increases every year, and agricultural ecological environment is also polluted. In this study, a neural network (NN) method was proposed based on red-green-blue (RGB) image to segment wheat ear spot field environment, then determine grade. Firstly, segmentation dataset single constructed provide benchmark for...
Background: Sheng-Mai Yin (SMY), a modern Chinese formula based on Traditional Medicine theory, has been used to treat cardiovascular diseases in Eastern Asia.Our study focuses the cardioprotection of SMY against doxorubicin (DOX)-induced cardiac toxicity vivo.Methods: Rats were injected with DOX (2.5 mg/kg) six injections over 2-week period.SMY was administrated intragastrically at dose 8.35, 16.7 and 33.4 g/kg, or g/kg only twice day concurrently for 2-weeks.A series assays performed...
Milk fat globule membrane (MFGM), which contains abundant glycoproteins and phospholipids, exerts beneficial effects on intestinal health immunomodulation. The aim of this study was to evaluate the protective possible underlying mechanisms MFGM cow's milk allergy (CMA) in a <i>β</i>-lactoglobulin (BLG)-induced allergic mice model. supplemented induced by BLG at dose 400 mg/kg body weight. Results demonstrated that alleviated food symptoms, decreased serum levels lipopolysaccharide,...
Oxanine (Oxa) is a deaminated base lesion derived from guanine in which the <i>N</i><sup>1</sup>-nitrogen substituted by oxygen. This work reports mutagenicity of oxanine as well DNA glycosylase (ODG) activities mammalian systems. Using human polymerase β, deoxyoxanosine triphosphate only incorporated opposite cytosine (Cyt). When an template, Cyt efficiently template oxanine; however, adenine and thymine are also Oxa with efficiency ∼80% Cyt/Oxa (C/O) pair. Guanine least efficiency, 16%...
Aims Asprosin is a newly discovered adipokine, it associated with the insulin resistance, lipid metabolism disorder, diabetes and obesity. However, there have been no reports on relationship between asprosin type 2 diabetic patients This study aims to investigate serum obesity in community. Materials methods A total of 491 Type Diabetes Mellitus (T2DM) were recruited from Zhuoma Community Care Station Chengbei West Street Service Center Changzhi City Shanxi Province November 2019 July 2021....
We have developed a method for intact mass analysis of detergent-solubilized and purified integral membrane proteins using liquid chromatography–mass spectrometry (LC–MS) with methanol as the organic mobile phase. Membrane detergents are separated chromatographically during isocratic stage gradient profile from 150-mm C3 reversed-phase column. The accuracy is comparable to standard methods employed soluble proteins; sensitivity 10-fold lower, requiring 0.2–5 μg protein. also compatible our...
Abstract Cyclooxygenase‐2 (COX‐2) overexpression is prominent in inflammatory diseases, neurodegenerative disorders, and cancer. Directly monitoring COX‐2 activity within its native environment poses an exciting approach to account for illuminate the effect of local environments on protein activity. Herein, we report development CoxFluor, first activity‐based sensing live cells with confocal microscopy flow cytometry. CoxFluor strategically links a natural substrate dye precursor engage both...
Detail features of magnetic resonance images play a cru-cial role in accurate medical diagnosis and treatment, as they capture subtle changes that pose challenges for doc-tors when performing precise judgments. However, the widely utilized naive diffusion model has limitations, it fails to accurately more intricate details. To en-hance quality MRI reconstruction, we propose comprehensive detail-preserving reconstruction method using multiple models extract structure detail k-space domain...
Abstract Background Airway epithelium is an important component of airway structure and the initiator remodeling in asthma. The changes extracellular matrix (ECM), such as collagen deposition structural disturbance, are typical pathological features remodeling. Thus, identifying key mediators that derived from capable modulating ECM may provide valuable insights for targeted therapy Methods datasets Gene Expression Omnibus database were analyzed to screen differentially expressed genes We...
Endonuclease V is an enzyme that initiates a conserved DNA repair pathway by making endonucleolytic incision at the 3' side one nucleotide from deaminated base lesion. Site-directed mutagenesis analysis was conducted seven motifs of thermostable Thermotoga maritima endonuclease to probe for residues affect DNA−protein interactions. Y80, G83, and L85 in motif III, H116 G121 IV, A138 V, S182 VI binding both double-stranded inosine-containing substrate nicked product, resulting multiple...
The enzyme endonuclease V initiates repair of deaminated DNA bases by making an endonucleolytic incision on the 3' side one nucleotide from a base lesion. In this study, we have used site-directed mutagenesis to characterize role highly conserved residues D43, E89, D110, and H214 in Thermotoga maritima catalysis. cleavage Mn2+-rescue analysis suggest that amino acid substitutions at D43 impede enzymatic activity severely while mutations E89 D110 may be tolerated. Mutations yield maintains...
Prostaglandin (PG) endoperoxide H synthase (PGHS)-2, also known as cyclooxygenase (COX)-2, can convert arachidonic acid (AA) to PGH2 in the committed step of PG synthesis. PGHS-2 functions a conformational heterodimer composed an allosteric (Eallo) and catalytic (Ecat) monomer. Here we investigated interplay between human (hu)PGHS-2 alternative COX substrate, endocannabinoid, 2-arachidonoylglycerol (2-AG), well stable analog, 2-O-arachidonylglycerol ether (2-AG ether). We compared inhibition...