A5085235406- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Birth, Development, and Health
- Gene Regulatory Network Analysis
- Epigenetics and DNA Methylation
- Prenatal Substance Exposure Effects
University of California, Los Angeles
2014
Stem cells, especially human embryonic stem cells (hESCs), are useful models to study molecular mechanisms of disorders that originate during gestation. Alcohol (ethanol, EtOH) consumption pregnancy causes a variety prenatal and postnatal collectively referred as fetal alcohol spectrum (FASDs). To better understand the events leading FASDs, we performed genome-wide analysis EtOH's effects on maintenance differentiation hESCs in culture. Gene Co-expression Network Analysis showed significant...
Molecular markers defining self-renewing pluripotent embryonic stem cells (ESCs) have been identified by relative comparisons between undifferentiated and differentiated cells. Most of analysis has done under a specific differentiation condition that may present significantly different molecular changes over others. Therefore, it is currently unclear if there are true consensus human ESCs (hESCs). To identify set key genes consistently altered during hESCs regardless conditions, we performed...
Potential teratogenic effects of alcohol on fetal development have been documented. Especially studies demonstrated deleterious effect ethanol exposure neuronal in animal models and the maintenance differentiation precursor cells derived from stem cells. To better understand molecular process neural differentiation, we performed gene expression microarray analysis human embryonic being directed to rosettes presence treatment. Here provide detailed experimental methods, information associated...