A5101737676- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Cardiomyopathy and Myosin Studies
- RNA Research and Splicing
- Renal and related cancers
- Cancer, Lipids, and Metabolism
- Amino Acid Enzymes and Metabolism
- RNA modifications and cancer
- Eicosanoids and Hypertension Pharmacology
- Enzyme function and inhibition
- Cancer Mechanisms and Therapy
- RNA and protein synthesis mechanisms
- Neuroscience and Neural Engineering
- HIV/AIDS oral health manifestations
- Molecular Biology Techniques and Applications
- Single-cell and spatial transcriptomics
- Pain Mechanisms and Treatments
- Oral and gingival health research
- Cell Image Analysis Techniques
- Genetic factors in colorectal cancer
- Muscle Physiology and Disorders
- Zoonotic diseases and public health
- Inflammatory mediators and NSAID effects
University of Louisville
2023-2025
The University of Texas Health Science Center at San Antonio
2024
Texas Biomedical Research Institute
2019-2024
The University of Texas at San Antonio
2024
Baylor College of Medicine
2005-2022
Texas Children's Hospital
2010-2022
Colorado State University
2020
Advanced Cell Diagnostics (United States)
2016-2020
The Bronx Defenders
2020
University of California, Irvine
2019
Biomarkers such as DNA, RNA, and protein are powerful tools in clinical diagnostics therapeutic development for many diseases. Identifying RNA expression at the single cell level within morphological context by situ hybridization provides a great deal of information on gene changes over conventional techniques that analyze bulk tissue, yet widespread use this technique setting has been hampered dearth automated ISH assays. Here we present an version technology RNAscope is adaptable to...
Abstract Intra-tumor heterogeneity (ITH) is a major underlying cause of therapy resistance and disease recurrence, read-out tumor growth. Current genetic ITH analysis methods do not preserve spatial context may detect rare subclones. Here, we address these shortfalls by developing validating BaseScope—a novel mutation-specific RNA in situ hybridization assay. We target common point mutations the BRAF , KRAS PIK3CA oncogenes archival colorectal cancer samples to precisely map morphological...
Since polycystic kidney disease (PKD) was first noted over 30 years ago to have neoplastic parallels, there has been a resurgent interest in elucidating neoplasia-relevant pathways PKD. Taking nontargeted metabolomics approach the B6(Cg)-Cys1(cpk/)J (cpk) mouse model of recessive PKD, we now characterized metabolic reprogramming these tissues, leading glutamine-dependent TCA cycle shunt toward total 2-hydroxyglutarate (2-HG) production cpk compared with B6 wild-type tissue. After...
Meloxicam is a nonsteroidal anti-inflammatory analgesic drug that often used in mice. However, doses of 1 to 5 mg/kg given twice daily were recently reported provide inadequate analgesia. Some studies suggest up 20 may be necessary for adequate pain management. We investigated the analgesia provided by high-dose meloxicam female CD1 Pharmacokinetic analyses demonstrated subcutaneous injection 10 or produced therapeutic plasma concentrations at least 12 h. Ovariectomies via ventral laparotomy...
Oct4 is a known master regulator of stem cell renewal and differentiation. Expression during differentiation regulated by promoter methylation the nucleosome remodeling histone deacetylation (NuRD) complex. Here, we show that Cdk2ap1, negative Cdk2 function cycle, promotes murine embryonic to down-regulate expression. We further this repressor Cdk2ap1 dependent on its physical interaction with methyl DNA-binding protein, Mbd3. Our data support potential molecular link between promoters,...
Feline immunodeficiency virus (FIV) is the domestic cat analogue of HIV infection in humans. Both viruses induce oral disease untreated individuals, with clinical signs that include gingivitis and periodontal lesions. Oral manifestations patients are abated by highly effective combination antiretroviral therapy (cART), though certain persist despite therapy. Microorganisms associated cavity opportunistic infections cause similar pathologies cats. To further develop this model, we evaluated...
Feline Immunodeficiency Virus (FIV) causes progressive immune dysfunction in cats similar to human immunodeficiency virus (HIV) humans. Although combination antiretroviral therapy (cART) is effective against HIV, there no definitive improve clinical outcomes with FIV. This study therefore evaluated pharmacokinetics and of cART (2.5 mg/kg Dolutegravir; 20 Tenofovir; 40 Emtricitabine) FIV-infected domestic cats. Specific pathogen free were experimentally infected FIV administered either or...
Molecular markers defining self-renewing pluripotent embryonic stem cells (ESCs) have been identified by relative comparisons between undifferentiated and differentiated cells. Most of analysis has done under a specific differentiation condition that may present significantly different molecular changes over others. Therefore, it is currently unclear if there are true consensus human ESCs (hESCs). To identify set key genes consistently altered during hESCs regardless conditions, we performed...
To evaluate the impact of American Heart Association (AHA) scientific statement regarding electrocardiograms (ECGs) and stimulant medications on practice community pediatricians.Retrospective evaluation survey analysis.Academic tertiary care center.Patients with ECGs referred to our institution by pediatricians an indication medication screening in year after AHA statement.We compared ECG ordering practices outcomes further estimated associated cost before statement.Abnormal findings,...
Mouse embryonic stem cells (mESCs) maintain pluripotency and indefinite self-renewal through yet to be defined molecular mechanisms. Leukemia inhibitory factor has been utilized the symmetrical of mESCs in culture. It suggested that molecules with significant cellular effects on retinoblastoma protein (pRb) or its related pathways should have functional impact mESC proliferation differentiation. However, involvement pRb pluripotent differentiation not extensively elaborated. In this paper,...
Kidney cancer is the sixth most common in United States, and its incidence increasing. The treatment of this malignancy took a major step forward with recent introduction targeted therapeutics, such as kinase inhibitors. Unfortunately, inhibition associated onset resistance after 1 to 2 years treatment. Regorafenib, like many multikinase inhibitors, was designed block activities several key pathways involved oncogenesis (Ras/Raf/MEK/ERK) tumor angiogenesis (VEGF-receptors), we have recently...
Atrial flutter is a rare complication of the insertion an umbilical venous catheter. We report neonate who had catheter placed within first 24 hours life and developed hemodynamically stable tachyarrhythmia. The arrhythmia was successfully managed with synchronized cardioversion, no further complications. presentation management are discussed. Physicians should be watchful for such potentially life-threatening complications from placement indwelling lines.
Potential teratogenic effects of alcohol on fetal development have been documented. Especially studies demonstrated deleterious effect ethanol exposure neuronal in animal models and the maintenance differentiation precursor cells derived from stem cells. To better understand molecular process neural differentiation, we performed gene expression microarray analysis human embryonic being directed to rosettes presence treatment. Here provide detailed experimental methods, information associated...
Drug discovery in whole-organisms such as zebrafish is a promising approach for identifying biologically-relevant lead compounds. However, high content imaging of at cellular resolution challenging due to the difficulty orienting larvae en masse that cell type interest clear view. We report development multi-pose method, which uses 96-well round bottom plates combined with standard liquid handler repose within each well multiple times, an image specific orientation can be acquired. have...
Clinical flow cytometry laboratories require quality control materials for assay development, validation, and performance monitoring, including new reagent lot qualification. However, finding suitable controls populations with uncommonly expressed antigens or rare populations, such as mast cells, can be difficult. To that end, we evaluated synthetic abnormal cell particles (SAMCP), developed together with, manufactured by, Slingshot Biosciences. The SAMCP's were designed to phenotypically...
In this study we present data to support the role for Cdk2ap2 in regulating self-renewal of mouse embryonic stem cells (mESCs) under permissive conditions, and cell survival during differentiation mESCs into terminally differentiated types. To understand function early development, generated with homozygous disruption endogenous locus (Cdk2ap2(tr/tr)). The Cdk2ap2(tr/tr) mESCs, when grown a complete growth medium containing leukemia inhibitory factor (LIF), showed an phenotype characterized...
It has been reported that nicotine/alcohol alter epigenetic control and lead to abrogated DNA methylation histone modifications, which could subsequently perturb transcriptional regulation critically important in cellular transformation. The aim of this study is determine the molecular mechanisms nicotine/alcohol-induced alterations their mechanistic roles human adult stem cells. We hypothesized induces deregulation machinery leads alterations, affect oral epithelial As an initiating step we...