A5100976816- Occupational and environmental lung diseases
- Cancer Research and Treatments
- Radiomics and Machine Learning in Medical Imaging
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Head and Neck Cancer Studies
- Cancer Immunotherapy and Biomarkers
- Cancer Genomics and Diagnostics
- Gene expression and cancer classification
- Single-cell and spatial transcriptomics
- Sarcoma Diagnosis and Treatment
- RNA Research and Splicing
- Cancer Cells and Metastasis
- Immunotherapy and Immune Responses
- Hepatitis B Virus Studies
- Medical Imaging and Pathology Studies
- Bioinformatics and Genomic Networks
- 3D Printing in Biomedical Research
- Breast Cancer Treatment Studies
- Pancreatic and Hepatic Oncology Research
- Plant responses to water stress
- Plant Molecular Biology Research
- Hepatitis C virus research
- CAR-T cell therapy research
Sun Yat-sen University
2025
Shanghai Jiao Tong University
2015-2025
Shanghai First People's Hospital
2016-2025
Ningxia Medical University
2025
Ningxia Medical University General Hospital
2025
First Affiliated Hospital of Zhengzhou University
2022-2025
The Fourth People's Hospital of Ningxia Hui Autonomous Region
2025
Shandong Agricultural University
2024
Peking Union Medical College Hospital
2011-2024
UPMC Hillman Cancer Center
2021-2024
Although distinct pathological stages of breast cancer have been described, the molecular differences among these are largely unknown. Here, through combined use laser capture microdissection and DNA microarrays, we generated in situ gene expression profiles premalignant, preinvasive, invasive human cancer. Our data reveal extensive similarities at transcriptome level progression suggest that alterations conferring potential for growth already present preinvasive stages. In contrast to tumor...
Abstract Introduction The importance of the tumor microenvironment in breast cancer has been increasingly recognized. Critical molecular changes stroma accompanying progression, however, remain largely unknown. We conducted a comparative analysis global gene expression stromal and epithelial compartments during progression from normal to preinvasive invasive ductal carcinoma. Methods combined laser capture microdissection microarrays analyze 14 patient-matched epithelium, stroma, epithelium...
Histamine is a multifunctional hormone that regulates smooth muscle contraction in the airways, acid secretion gut, and neurotransmitter release central nervous system through three well characterized receptor subtypes, H(1), H(2), H(3), respectively. As part of directed effort to discover novel G-protein-coupled receptors homology searching genomic databases, we identified partial clone (GPCR105) had significant recently histamine H(3) cDNA. Expression full-length human GPCR105 cells...
Kinase inhibitors constitute an important new class of cancer drugs, whose selective efficacy is largely determined by underlying tumor cell genetics. We established a high-throughput platform to profile 500 lines derived from diverse epithelial cancers for sensitivity 14 kinase inhibitors. Most were ineffective against unselected but exhibited dramatic killing small nonoverlapping subsets. Cells with exquisite EGFR, HER2, MET, or BRAF marked activating mutations amplification the drug...
Histologic tumor grade is a well-established prognostic factor for breast cancer, and grade-associated genes are the common denominator of many gene signatures. The objectives this study as follows: (a) to develop simple expression index (molecular or MGI), (b) determine whether MGI our previously described HOXB13:IL17BR together provide improved information.From published list whose correlates with both stage progression, we selected five cell cycle-related build evaluated in two publicly...
Human papillomavirus (HPV) is established as causative in oropharyngeal squamous cell carcinomas (OSCCs), being detected 50% to 80% of tumors by DNA situ hybridization (ISH) and/or polymerase chain reaction. However, these tests do not assess viral transcription. Many consider E6/E7 messenger ribonucleic acid (mRNA) the best indicator HPV status, but it has been OSCC. We constructed tissue microarrays (TMAs) from a cohort OSCC for which p16 immunohistochemistry and ISH were previously...
Abstract Microchannel networks within engineered 3D scaffold can allow nutrient exchange and rapid blood vessels formation. However, fabrication of a bone microenvironment‐mimicking with hierarchical micro/nanofibrous microchannel structures is still challenge. Herein, inspired by structural functional cues remodeling, networks‐enriched nanofibrous using printing thermally induced phase separation techniques, which facilitate cells migration nutrients transportation, developed. The...
The favorable microstructure and bioactivity of tissue-engineered bone scaffolds are closely associated with the regenerative efficacy defects. For treatment large defects, however, most them fail to meet requirements such as adequate mechanical strength, highly porous structure, excellent angiogenic osteogenic activities. Herein, inspired by characteristics a "flowerbed", we construct short nanofiber aggregates-enriched dual-factor delivery scaffold via 3D printing electrospinning...
This report demonstrates that a rapid decrease of peripheral T cell subsets is unique characteristic in patients with SARS during acute infection, although total white blood counts, red and platelet counts remain relatively normal. In recovering patients, dramatic restoration was seen the periphery. Although underlying mechanism observed stage remains unknown, this clinical can facilitate an earlier more accurate diagnosis SARS.
We previously identified three genes, HOXB13, IL17BR and CHDH, the HOXB13:IL17BR ratio index in particular, that strongly predicted clinical outcome breast cancer patients receiving tamoxifen monotherapy. Confirmation larger independent patient cohorts was needed to fully validate their utility.Expression of IL17BR, estrogen receptor (ER) progesterone (PR) were quantified by real-time polymerase chain reaction 852 formalin-fixed, paraffin-embedded primary cancers from 566 untreated 286...
The application of genome-wide expression profiling to determine how drugs achieve their therapeutic effect has provided the pharmaceutical industry with an exciting new tool for drug mode-of-action studies. We used DNA chip technology study cellular responses perturbations ergosterol biosynthesis caused by broad-spectrum antifungal agent itraconazole. Simultaneous examination over 6,600 Candida albicans gene transcript levels, representing entire genome, upon treatment cells 10 microM...
Abstract Context.—Correct diagnosis of the tissue origin a metastatic cancer is first step in disease management, but it frequently difficult using standard pathologic methods. Microarray-based gene expression profiling has shown great promise as new tool to address this challenge. Objective.—Adoption microarray technologies clinic remains limited. We aimed bridge technological gap by developing real-time quantitative polymerase chain reaction (RT-PCR) assay. Design.—We constructed database...
Abstract Context.—Correct diagnosis of the tissue origin a metastatic cancer is first step in disease management, but it frequently difficult using standard pathologic methods. Microarray-based gene expression profiling has shown great promise as new tool to address this challenge. Objective.—Adoption microarray technologies clinic remains limited. We aimed bridge technological gap by developing real-time quantitative polymerase chain reaction (RT-PCR) assay. Design.—We constructed database...
Abstract Introduction. This retrospective, multi-institutional study evaluated the accuracy of tissue-of-origin prediction by molecular profiling in patients with carcinoma unknown primary site (CUP). Methods. Thirty-eight 501 (7.6%) CUP, seen 2000–2008, had their latent tumor subsequently identified during life. Twenty-eight these (73.7%) adequate initial tissue biopsies available for a reverse transcriptase-polymerase chain reaction (RT-PCR) assay (Cancer Type ID; bioTheranostics, Inc.,...
Multiple factors including long-term treatment with tamoxifen are involved in the development of selective estrogen receptor (ER) modulator resistance ERα-positive breast cancer. Many underlying molecular events that confer known but a unifying theme is yet to be revealed. In this report, we provide evidence HOXB7 overexpression renders MCF-7 cells resistant via cross-talk between tyrosine kinases and ERα signaling. an ERα-responsive gene. Extended resulted progressively increasing levels...
Abstract Inflammation in the colon contributes significantly to colorectal cancer development. While aspirin reduces risk, its action mechanism, especially inflammation tumor microenvironment, still remains obscure. Here, we examined this issue by subjecting mice deficient each prostaglandin (PG) receptor colitis-associated model. Deficiency of PGE subtype EP2 selectively reduced, and deficiency EP1 EP3 enhanced, formation. is expressed infiltrating neutrophils tumor-associated fibroblasts...
T helper 1 (Th1) cells have critical roles in various autoimmune and proinflammatory diseases. cAMP has long been believed to act as a suppressor of IFN-γ production Th1 cell-mediated immune inflammation. Here we show that actively promotes differentiation by inducing gene expression cytokine receptors involved this process. PGE2 signalling through EP2/EP4 mobilizes the cAMP-PKA pathway, which induces CREB- its co-activator CRTC2-mediated transcription IL-12Rβ2 IFN-γR1. Meanwhile,...
Biomarkers such as DNA, RNA, and protein are powerful tools in clinical diagnostics therapeutic development for many diseases. Identifying RNA expression at the single cell level within morphological context by situ hybridization provides a great deal of information on gene changes over conventional techniques that analyze bulk tissue, yet widespread use this technique setting has been hampered dearth automated ISH assays. Here we present an version technology RNAscope is adaptable to...