INTRODUCTION: A novel coronavirus (severe acute respiratory syndrome-related 2 (SARS-CoV-2)) emerged in December 2019 and rapidly spread over the world having provoked a pandemic of disease. This highly pathogenic virus can attack lung tissue derange gas exchange leading to distress syndrome systemic hypoxia. Hypoxic conditions trigger activation adaptation mechanisms including hypoxia-inducible factor-1á (HIF-1á) which is involved regulation key processes, e. g, proliferation metabolism...

Background: The aim of this work is to study the significance carnitine fractions and NO metabolites for assessment spermatozoa resistance cryopreservation, which purpose these parameters were investigated in seminal plasma animals divided according indices stallions subjected cryopreservation. Methods: There 18 breeding with an average age 11.28±5.46 years, all Arabian breed. Using cluster analysis indicators semen quality, identified into two groups: high low sperm...

Introduction. Recent work demonstrates that HIF-1α and NOS isoforms are detectable in plasma, representing their potential practical relevance as biomarkers. At the same time, data on stability of these proteins plasma contradictory. Aim study. To investigate amount iNOS patients with COVID-19 infection different severity to assess possi-bilities adaptation hypoxia. Material methods. The pilot study involved 50 divided into three groups: 1) 17 moderate disease; 2) pa-tients severe condition;...

Рязанский государственный медицинский университет имени

INTRODUCTION: The study of markers oxidative stress, metabolites nitric oxide (II) (NOx) and the balance carnitine fractions in mitochondria rat epididymis, permits to evaluate protective role L-arginine experimental hyperhomocysteinemia.
 AIM: To influence on parameters energy metabolism, level NO metabolites, modification proteins head tail epididymis MATERIALS AND METHODS: In animals group 1 (n = 8), severe hyperhomocysteinemia (HHcy) was modeled by administration methionine...

Aim. To study the concentration of succinate and activity dehydrogenase (SDH) mononuclear blood leukocytes as markers rapid adaptation mitochondria to hypoxia in patients with exacerbation chronic obstructive pulmonary disease (COPD).
 Materials Methods. The involved 58 COPD 13 conventionally healthy volunteers 40-75 years age. In accordance GOLD 2018 principles complex assessment, were divided groups B (n=18), C (n=20), D (n=20) comparable age, FEV1 pack-of-cigarettes/year index....

Hyperhomocysteinemia is a risk factor for many human diseases, including pulmonary pathologies. In this context much interest attracts secondary mitochondrial dysfunction, which an important link in pathogenesis of diseases associated with hyperhomocysteinemia. The study was conducted using male Wistar rats. It found that under conditions severe hyperhomocysteinemia caused by administration methionine, homocysteine accumulated lung mitochondria thus suggesting direct toxic effect on these...

Проблема коморбидности приобретает все большую актуальность в клинической практике.С каждым годом растет популяция больных с сочетанной патологией.Закономерно увеличение количества заболеваний у одного пациента старшей возрастной группе.Под «коморбидностью» подразумевают наличие больного двух взаимосвязанных заболеваний.При патологии неоспорима необходимость изучения особенностей патогенетического взаимодействия заболеваний, внедрения новых методов диагностики, разработка индивидуальных...

Aim. To evaluate the clinical and pathogenetic value of nitric oxide metabolites measurement in blood patients with bronchial asthma essential hypertension. 
 Methods. The study included 91 patients. Three groups were identified: (30 patients, group 1), combination hypertension (31 2), 3). Measurement level total patients’ was performed use spectrophotometric method light spectrum based on Griess reaction. Results. median had highest accounted for 75.6 [70.4; 80.8] umol/L. Level lower,...

Aim. To study the levels of nitric oxide (II) metabolites, lactate, endogenic carnitine and mitochondrial oxidoreductase activity in epididymis tissue at low synthesis and, based on obtained data, to assess functional condition rat mitochondria. Methods. 16 Wistar rats were allocated two equal groups: The first group was administered L-NG-nitroarginine methyl ester (L-NAME), which is non-selective NO-synthase inhibitor, a dose 25 mg/kg for 7 days. second (the control group) got 0.9% sodium...

Звягина В.И. 1 , Бельских Э

INTRODUCTION: Patients with chronic obstructive pulmonary disease (COPD) have a characteristic problem of comorbid and combined pathology, which impedes the objective assessment severity patients’ condition. Of special attention is COPD in patients overweigh obesity, since adipose tissue not only worsens dysfunction, but indirectly enhances oxidative damage to cells, especially hypoxic conditions. Therefore, level markers that directly characterize degree distress, interesting as possible...

Aim. To study the indicators of energy metabolism and oxidative stress in mononuclear leukocytes peripheral blood to assess possibility mitochondrial dysfunction development chronic obstructive pulmonary disease bronchitis.
 Methods. The included 50 patients aged 40 75 years with (COPD) or bronchitis. first group 13 In accordance GOLD spirometric classification, second third groups COPD moderate severity (COPD 2) (n=17) severe 3) (n=20) respectively. isolated leukocytes, activity...

<b>Background:</b> in the formation of asthma and essential hypertension (EH) there is role nitric oxide (NO), which synthesis by NO-synthases (NOS). NOS genes polymorphism can affect NO production play pathogenesis comorbidity. <b>Aims:</b> to study comorbidity EH effect on level blood exhaled air. <b>Methods:</b> Our recruited 91 patients with EH. All were genotyped for NOS3 786C/T (rs2070744) polymorphism, measured plasma metabolites Griess reaction, FeNO NObreath (Cosmed, Italy)....

Hyperhomocysteinemia is a risk factor for many diseases, including reproductive disorders in men. L-carnitine used medical practice to correct impaired bioenergetic conditions; patients with idiopathic forms of infertility its effects are associated improvement the sperm parameters. However, effect exogenous on level homocysteine gonadal tissues, as fertility, has not been investigated yet. The aim this study was investigate activity enzymes epididymal mitochondrial fraction, dynamics...

Aim. To study the effect of carnitine chloride on functional state mitochondria and endogenous content in conditions L-NAME-induced decrease nitric oxide synthesis (II). Materials methods. Twenty four Wistar rats were divided into following groups: control group, group with intraperitoneal introduction L-NAME dose 25 mg/kg for 7 days receiving by analogous scheme against background 300 21 days. The animals experienced lactate dehydrogenase (LDG), succinate (SDG), superoxide dismutase (SOD),...

Study of thrombolytic therapy and concomitant antiplatelet treatment in myocardial infarction with ST-segment elevation stay a very actual problem. The article presents the analysis effecacy safety thrombolysis background ticagrelor during first 24 h. Ticagrelor intake were accompanied by significant reduction frequency reperfusion arrhythmias comparison clopidogrel composition therapy.

Background. Chronic obstructive pulmonary disease and bronchial asthma, when combined in one patient, are characterized by a low level of control. Excess weight aggravates the course diseases. The study features this syntropy will improve effectiveness therapeutic measures. Aim. cytokines carbonylated proteins patients with combination asthma chronic overweight normal during an exacerbation. Material methods. included 136 people: first group (n=30), second (n=36), third (n=29), fourth...

ОЦЕНКА ВЗАИМОСВЯЗИ МЕЖДУ БАЛАНСОМ ФРАКЦИЙ КАРНИТИНА И МЕТАБОЛИТАМИ NO (

<b>Introduction:</b> The mitochondrial membrane potential (Δψ) and level of carbonylated proteins in mononuclear leukocytes peripheral blood (ML) is interest as a possible marker systemic dysfunction COPD. <b>Aim:</b> Evaluation the indicators secondary ML smokers ex-smokers with exacerbation <b>Methods:</b> study included 25 people. control group consisted 8 healthy volunteers. 17 patients non-infectious COPD were divided into groups comparable age: (n=9) (n=8). Blood was taken on second...

<b>Introduction:</b> The increase in the incidence of bronchial asthma, COPD and obesity causes a high probability their combination one patient. activity systemic inflammation each diseases is able to modify clinical picture, which makes assessment effect excess body weight on level markers patients with asthma-COPD overlap (ACO) relevant. <b>Aim:</b> To estimate pro-inflammatory cytokines ACO normal during exacerbation disease. <b>Methods:</b> study included 30 period, (mean age 60.5±4.7),...