A5071875283- Barrier Structure and Function Studies
- Enzyme Production and Characterization
- Connexins and lens biology
- Synthesis and Catalytic Reactions
- Gut microbiota and health
- Connective tissue disorders research
- Protease and Inhibitor Mechanisms
- Protein Hydrolysis and Bioactive Peptides
- Pancreatic function and diabetes
- Insect Resistance and Genetics
- Viral Infectious Diseases and Gene Expression in Insects
- Fibroblast Growth Factor Research
- Eicosanoids and Hypertension Pharmacology
- Bee Products Chemical Analysis
- Probiotics and Fermented Foods
- Signaling Pathways in Disease
- S100 Proteins and Annexins
- Biochemical effects in animals
- Enzyme function and inhibition
- Collagen: Extraction and Characterization
- Blood Coagulation and Thrombosis Mechanisms
- Sphingolipid Metabolism and Signaling
- Bone and Dental Protein Studies
Shanghai Ocean University
2023-2025
Shanghai Innovative Research Center of Traditional Chinese Medicine
2023
Tokyo University of Marine Science and Technology
2022
Collagen and its peptides exhibit remarkable antioxidant activity, superior biocompatibility, water solubility, making them a significant research focus in skin care. Hence, the recombinant humanized collagen types I, III, XVII complexed with niacinamide were developed to address damage human foreskin fibroblasts (HFF-1) caused by ultraviolet radiation evaluate basement membrane proteins rat model. The Cell Counting Kit-8 (CCK-8) assay showed that higher concentrations of complex increased...
The functional exploration of marine-derived proteins is at the forefront nutritional research. Argentine squid protein (ASP) was extracted from carcasses and hydrolyzed using neutral protease, with degree hydrolysis serving as response variable. Using single-factor experiments surface methodology, we identified optimal conditions for preparing peptides (ASPP). reached 41.32% ± 0.27 under 7% enzyme preparation addition, 2.4 h digestion time, 6% substrate concentration. ASPP subsequently...
Tight junctional complexes (TJCs) between cerebral microvascular endothelial cells (CMECs) are essential parts of the blood–brain barrier (BBB), whose regulation closely correlates to BBB’s integrity and function. hCMEC/D3 is typical cell line used imitate investigate function BBB via construction an in vitro model. This study aims protective effect deep-sea-derived fibrinolytic compound FGFC1 against H2O2-induced dysfunction TJCs elucidate underlying mechanism. The was shown decline...
This study aimed to investigate the protective effect of metabolites produced by a new Lactiplantibacillus plantarum strain BF1-13, isolated from deep seawater (DSW), on intestinal epithelial barrier against dysfunction induced hydrogen peroxide (H2O2) and elucidate mechanism underlying effect. Protective BF1-13 function model treated with H2O2 was investigated transepithelial electrical resistance (TEER). The enhanced Claudin-4 (CLDN-4) expression, including at transcription level,...
One of the supporting factors behind biomolecules recently used in anti-aging and skin nourishment is their antioxidant properties. Hydrogen peroxide (H2O2) a well-known small molecule oxidant that induces apoptosis human fibroblast (HSF) cells through synthesis inflammatory cytokines. Hence, this study aimed to investigate activities protein hydrolysates prepared from Iris squid (Symplectoteuthis oualaniensis) (PHCSO) vitro. Firstly, two peptides with MWs more than 10 kDa (PHCSO-1) less...
The fibrinolytic kinase identified in the nereid worm (Perinereis aibuhitensis Grub) displays exceptional activity, stability, and specificity, suggesting its potential as a promising candidate for advancement of new thrombolytic drugs. In this study, process was optimized production using Escherichia coli, effects factors such inoculum, pH, OD, temperature, inducer concentration, time on protein yield were investigated. optimum points key parameters determined by single-factor experiments,...
As an essential thrombolytic agent, the tissue plasminogen activator receives increasing attention due to its longer half-life, lower immunogenicity, and easier administration, which are superior other agents. In this study, isolated purified from sandworm (
The thrombin-activatable fibrinolysis inhibitor (TAFI) is an important regulator in the balance between blood clot formation (coagulation) and dissolution (fibrinolysis), which mainly activated by thrombin bonded with thrombomodulin (TM).
Glucokinase (GK), as a key enzyme regulating carbohydrate metabolism, has been widely researched therapeutic target for diabetes.This study aimed to investigate the influence of magnesium ion concentration, ATP and concentration on catalytic activity GK by response surface.A cellular model insulin resistance (IR) was established observe ameliorating effect IR screening duration action.The results indicated that optimal concentrations were found be 1.1 mM ion, 1.4 ATP, 0.5 U/mL glucokinase.In...