A5052566755- MicroRNA in disease regulation
- Hemoglobinopathies and Related Disorders
- Cancer-related molecular mechanisms research
- Erythrocyte Function and Pathophysiology
- RNA modifications and cancer
- Digital Imaging for Blood Diseases
- Advanced oxidation water treatment
- Iron Metabolism and Disorders
- Radioactive element chemistry and processing
- Bacterial biofilms and quorum sensing
- Blood groups and transfusion
- Blood properties and coagulation
- Antiplatelet Therapy and Cardiovascular Diseases
- Venous Thromboembolism Diagnosis and Management
- Electrochemical Analysis and Applications
- Gut microbiota and health
- Vibrio bacteria research studies
- Plant-Derived Bioactive Compounds
- Cell death mechanisms and regulation
- RNA Research and Splicing
- Helicobacter pylori-related gastroenterology studies
- Chemical Analysis and Environmental Impact
- Listeria monocytogenes in Food Safety
- Natural product bioactivities and synthesis
- Systemic Lupus Erythematosus Research
Prince of Songkla University
2017-2025
Mahidol University
2009-2022
ABSTRACT Bacterial communication system known as quorum sensing (QS) is a pivotal for bacterial survival, adaptation and pathogenesis. Members in the multicellular community may synthesize or acquire signaling molecule order to elicit downstream cellular processes. Roles of indole derivatives, new class quorum-sensing signal molecules, various physiologies virulence have been reported recently. Indole normally found mammal gastrointestinal tract metabolite tryptophan metabolism by...
Familial adenomatous polyposis (FAP) is an autosomal dominant syndrome associated with germline mutations in the coli (APC) gene. Patients eventually may develop colorectal cancer (CRC) if they are not diagnosed early stages. Dysbiosis important contributing factor to complex events carcinogenesis, which poorly understood. First, 25 tissue samples from 13 patients FAP at Songklanagarind Hospital were classified as nontumor (n = 18) or tumor tissues 7). Following isolation, 5 DNA of...
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Background β-thalassemia occurs from the imbalanced globin chain synthesis due to absence or inadequate β-globin production. The excessive unbound α-globin chains precipitate in erythroid precursors and mature red blood cells leading ineffective erythropoiesis hemolysis.Design Methods In vitro reticulocytes different types of thalassemic mice was performed. effect assessed changes cell properties including increased numbers vesicles apoptotic cells, reactive oxygen species decreased...
Abstract β-Thalassaemia results from defects in β-globin chain production, leading to ineffective erythropoiesis and subsequently severe anaemia other complications. Apoptosis autophagy are the main pathways that regulate balance between cell survival death response diverse cellular stresses. Herein, of erythroid lineage cells bone marrow both β IVS2-654 -thalassaemic mice β-thalassaemia/HbE patients was investigated. Phosphatidylserine (PS)-bearing basophilic erythroblasts...
β-Thalassemia (β-thal), is an inherited blood disorder caused by reduced or absent synthesis of β-globin chains leading to imbalance globin chain synthesis. The clearance β-thalassemic abnormal red cells (RBCs) that result from excessive unbound α-globin mainly achieved activated monocytes. phagocytic activity β-thal monocytes significantly increases when co-cultured with normal and RBC individuals compare RBCs. present study indicates microRNA (miR) plays a role in monocyte activation. In...
Abstract Background The Mindray BC‐5000Vet hematology analyzer is a flow cytometry‐based automated that generates complete blood count with five‐part white cell (WBC) differential count. Objectives We aimed to validate reliability results of the for use in dog and cat blood. Methods Imprecision was performed using manufacturer’s quality control material at low, normal, high levels. Blood sample healthy ill dogs cats were studied comparability between manual methods analyzer. Forty 40...
The phorbol 12-myristate 13-acetate (PMA)-induced U937 cell line has been widely used as an in vitro model for studying the functions of human macrophages. However, there are several concentrations PMA commonly to drive differentiation monocytic macrophage. Also, expression microRNA-155 (miR-155) and miR-125b PMA-treated not yet reported. five usual stimulating macrophage 10, 25, 50, 100, 200 nM. In this study we compared levels miR-155, their related genes involved U937-derived cells after...
Thalassemia is a genetic disorder, occurring because of an imbalance in the globin chain production. Oxidative stress erythroid cells thalassemia mainly generated from excess chains, by Fenton reaction, leading to hemolysis and ineffective erythropoiesis. Previously, data has shown that microRNAs (miRNAs) are involved oxidative regulation red blood (RBCs). microR-214 been reported respond with external modulating activating transcription factor 4 (ATF4). In this study, we illustrated...
Protein O-linked-N-acetylglucosaminylation (O-GlcNAcylation) is a dynamic post-translational modification process that plays an essential role in biological activities. Growing evidence indicates aberration of O-GlcNAcylation associated with various diseases, e.g. diabetes, neurological and cancers. However, the mechanistic studies are lagging behind other modifications due to its extremely low abundance, limited analytical tools, specificity. Herein, diagonal strong cation exchange...
Oxidative damage to erythroid cells plays a key role in the pathogenesis of thalassemia. The oxidative stress thalassemia is potentiated by heme, nonheme iron, and free iron produced Fenton reaction, due degradation unstable hemoglobin overload. In addition, levels antioxidant enzymes molecules are significantly decreased erythrocytes α- β-thalassemia. control red blood (RBCs) known be mediated microRNAs (miRNAs). cells, microR-214 (miR-214) has been reported respond external stress....
Background Ineffective erythropoiesis (IE) is the primary cause of anemia and associated pathologies in β -thalassemia. The characterization IE imbalance erythroid proliferation differentiation, resulting increased erythroblast that fails to differentiate gives rise enucleate RBCs. MicroRNAs (miRs) are known play important roles hematopoiesis. miR-155 a multifunctional molecule involved both normal pathological hematopoiesis, its upregulation observed patients with -thalassemia/HbE. However,...
Ethidium bromide, which is commonly applied in molecular biological applications, a mutagenic agent demanding proper treatment. In the present study, magnetic fraction of fly ash, prepared from ASTM class F material, was used as catalyst for heterogeneous Fenton process degrading ethidium bromide aqueous solution. The dosage catalyst, initial pH solution, and concentration hydrogen peroxide significantly influenced removal rate. Under optimum conditions (2 mmol/L 100 g/L ash at 3), an...
Fly ash was used as a heterogeneous Fenton catalyst to degrade methylene blue in aqueous solution. The influence off key operating parameters on the degradation efficiency, including concentrations of hydrogen peroxide and blue, dosage, initial pH, were studied. optimal conditions 2 mmol/L peroxide, 4 mg/mL fly ash, pH 3. Under these conditions, efficiency approximately 93% within h. by heterogenous process followed pseudo-first order kinetics activation energy shows that reaction is...
Abstract Macrophages play essential roles in erythrophagocytosis and iron recycling. β‐thalassemia is characterized by a genetic defect hemoglobin synthesis, which increases the rate of We previously showed that reduced expression BTB CNC homolog 1 (BACH1) gene leads to increased phagocytosis abnormal RBCs activated monocytes. However, mechanisms underlying this RBC clearance remained unclear. Herein, spleen bone marrow cells β‐thalassemic mice were examined for CD markers iron‐recycling...
The regulation of globin gene expression is significantly important to understand the pathogenesis disorders. Recent findings have shown that microRNAs (miRNAs, miRs) play an role in expression. miR-144 erythroid lineage-specific miRNA, which its mediates NRF2 silencing and inhibits fetal hemoglobin However, roles other genes especially ɑ-globin cluster remain unknown. This study, thus, examined functional studies K562 human cell line. results revealed z-globin were silenced by overexpressed...
The erythrocyte sedimentation rate (ESR) analyser is widely used in haematological testing. In addition to the Westergren method, new automatic methods for ESR measurements have been developed. We aimed study reliability, precision, accuracy and stability of Caretium XC-A30 automated analyser.Ethylenediamine tetraacetic acid (EDTA)-treated blood samples were analysed via method compare accuracy. Precision was assessed using control patient classified into three groups-low, medium...
Objective: Heparins can interfere with lupus anticoagulant (LA) testing, especially when the detection assays do not contain a heparin neutralizer. In this study we evaluated whether hexadimethrine bromide commonly known as polybrene was able to neutralize effects of unfractionated (UFH) on activated partial thromboplastin time (aPTT)-based LA determination. Material and Methods: The influence aPTT-based testing results 14 patients receiving UFH therapy studied. Measurements were performed...