A5052926194- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogenic Bacteria Studies
- Cocoa and Sweet Potato Agronomy
- Genetic and phenotypic traits in livestock
- Plant Pathogens and Fungal Diseases
- Plant Disease Management Techniques
- Animal Nutrition and Physiology
- Plant Disease Resistance and Genetics
- Berry genetics and cultivation research
- Insect-Plant Interactions and Control
- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Animal Behavior and Welfare Studies
- Insect symbiosis and bacterial influences
- Viral gastroenteritis research and epidemiology
- Phytochemical compounds biological activities
- Agriculture and Biological Studies
- Phytase and its Applications
- Plant Pathogens and Resistance
- Chronic Kidney Disease and Diabetes
- Animal Virus Infections Studies
- Rabbits: Nutrition, Reproduction, Health
- Effects of Environmental Stressors on Livestock
Czech Academy of Sciences, Biology Centre
2015-2025
Czech Academy of Sciences
2015-2025
Institute of Plant Molecular Biology
2015-2025
Institute of Animal Science
2007-2015
Ústav pro Péči o Matku a Dítě
1979
Virus diseases of strawberry present several complex problems. More than 25 viruses have been described in the genus Fragaria thus far. Here, we describe a novel rhabdovirus, tentatively named virus 1 (StrV-1), that infects F. ananassa and vesca plants. Genomic sequences three distinct StrV-1 genotypes co-infecting single host were obtained using combined Illumina Ion Proton high-throughput sequencing. was transmitted to herbaceous plants via Aphis fabae A. ruborum, further mechanically...
Apple viruses pose significant threat to global apple production. In this study, HTS technology was used investigate the virome in Czech Republic. Previously reported viruses, including ACLSV, ASPV, ASGV, ApMV, AGCaV, and CCGaV, were confirmed, near-complete genomes assembled. Additionally, two novel ARWV1 ARWV2 identified for first time Phylogenetic analyses showed low genetic variability among isolates, suggesting a possible recent introduction or limited diversification. contrast,...
A novel RNA virus infecting strawberry plants was discovered using high-throughput sequencing. The analyzed plant simultaneously infected with three different genetic variants of the virus, provisionally named (StrVA). Although StrVA is phylogenetically clustered several recently discovered, unclassified viruses, it has a smaller genome and unique features in its genomic organization. specific sensitive qPCR system for detection identified designed. survey conducted Czech Republic revealed...
Members of the genus Luteovirus are responsible for economically destructive plant diseases worldwide. Over past few years, three luteoviruses infecting Prunus trees have been characterized. However, biological properties, prevalence, and genetic diversity those viruses not yet studied. High-throughput sequencing samples various wild, cultivated, ornamental species enabled identification four novel in which we obtained complete or nearly genomes. Additionally, identified another new putative...
The breeding value (EBV) of Holstein cattle milk performance from the first lactation was evaluated using a regular Animal Model or by Single-Step Prediction genomic (GEBV). A total 838 bulls were genotyped Illumina BovineSNP50 Beadchip V2. Two overlapping sets performances evaluated: calving years 1991-2004, with 729 341 lactations and 1 394 487 animals in pedigree 1996-2009, 808 436 608 pedigree. older data set included 526 bulls, which daughters' known for 210 individuals. All newer set....
During their lifetime, perennial woody plants are expected to face multiple infection events. Furthermore, genotypes of individual virus species may co-infect the same host. This eventually lead a situation where harbor complex communities viral species/strains. Using high-throughput sequencing, we describe co-infection sweet and sour cherry trees with diverse genomic variants two closely related viruses, namely prunus F (PrVF) (CVF). Both viruses most homologous members Fabavirus genus...
Five isolates of a new member the family Closteroviridae, tentatively named blackcurrant leafroll-associated virus 1 (BcLRaV-1), were identified in currant. The 17-kb-long genome codes for 10 putative proteins. replication-associated polyprotein has several functional domains, including papain-like proteases, methyltransferase, Zemlya, helicase, and RNA-dependent RNA polymerase. Additional open reading frames code small protein predicted to integrate into host cell wall, heat-shock 70...
Estimated breeding values and genomic enhanced for milk production of young genotyped Holstein bulls were predicted using a conventional animal model, ridge regression prediction procedure, best linear unbiased prediction, single-step one-step blending procedures. For the nation-wide database domestic Czech records was combined with deregressed proofs from Interbull files through 2008, which had been transformed by multiple across country evaluation to reflect conditions. 1259 already proven...
The changes of insulin, blood sugars, lactate and free fatty acid levels were studied in 12 newborns diabetic mothers 21 healthy the course intravenous infusions glucose galactose at a dose 0.5 g.kg-1.h-1. During infusion striking increase insulin took place, which was higher infants mothers. Galactose administration caused only mild insulinemia 1st h infusion. In increased during 1 st but decreased afterwards. lasted for whole time decrease present both sugars. results show that is quickly...
Before being used for insemination, young bulls of Czech Fleckvieh (CF) are tested growth at performance-test stations. While stations, the weighed monthly. Evaluation included 7 448 with 82 676 records weight measured from 6 to 520 days life. In station-year-period (HYS), which can be prolonged up 3 months, different groups were according beginning curve and test-days weighing. Weight analyses handle heterogeneous variability based on age. Legendre Polynomials (LP) 5 parameters described...
6 508 bulls of dual-purpose cattle at performance-test stations were weighed intervals 30 days from birth to 420 days. After all editing a total 74 558 weight records available. Live was evaluated by orthogonal Legendre Polynomial (LP) degree 4 and Linear Spline function (SP) with 5 knots. The fixed effects test-day-year-station weighing (TDS) LP within station-year explain together 97% variability. Variance components estimated REML (REMLF90 programme) taking into account heterogeneous...
The intraspecies variability of gooseberry vein banding associated virus (GVBaV) was analyzed by using 5 complete and 9 partial sequences compared with other badnavirus species. GVBaV recognized to be a monophyletic very homogeneous species up 94% identities in distinct proteins. Analysis non-synonymous synonymous substitution ratios (dNS/dS) revealed higher values for ORF4 comparison genes. This could reflect different evolutionary pressure upon this ORF. A highly variable region possible...
Virus diseases of strawberry present several complex problems. More than 25 viruses have been described in the genus Fragaria thus far. Here, we describe a novel rhabdovirus, tentatively named virus 1 (StrV-1), that infects F. ananassa and vesca plants. Genomic sequences three distinct StrV-1 genotypes co-infecting single host were obtained using combined Illumina Ion Proton high-throughput sequencing. was transmitted to herbaceous plants via Aphis fabae A. ruborum, further mechanically...
Apple hammerhead viroid (AHVd) was detected in the apple cultivar ‘Šampion’ and mixed infection with Solanum nigrum ilarvirus 1 (SnIV-1) cultivars ‘Selena’ ‘Jonagored Supra’, using a high-throughput sequencing method. Experiments were conducted to eliminate both pathogens apples meristem tip cultures combination antivirotics ribavirin, rimantadine, zidovudine. Elimination of verified by repeated RT-PCR qRT-PCR assays after 7–11 months. SnIV-1 from all successful each three at concentrations...
Raspberry plants, valued for their fruits, are vulnerable to a range of viruses that adversely affect yield and quality. Utilizing high-throughput sequencing (HTS), we identified novel virus, tentatively named raspberry enamovirus 1 (RaEV1), in three distinct plants. This study provides comprehensive characterization RaEV1, focusing on its genomic structure, phylogeny, possible transmission routes. Analysis nearly complete genomes from 14 RaEV1 isolates highlighted regions variance,...
A collection of highbush blueberry (Vaccinium corymbosum L.) cultivars planted in the field for propagation South Bohemia was surveyed May and July 2009 occurrence detrimental viruses. total 67 plants 10 (Berkeley, Burlington, Blue Crop, Bluetta, Darrow, Duke, Gila, Jersey, Late Blue, Northland), were observed typical Blueberry red ringspot virus (BRRV) symptoms that appear as reddish ring spots blotches on stems fruits, exclusively upper surface older leaves but not underside. Samples...