A5011071152- Mesenchymal stem cell research
- Cancer Cells and Metastasis
- Osteoarthritis Treatment and Mechanisms
- Tissue Engineering and Regenerative Medicine
- Cancer, Hypoxia, and Metabolism
- Shoulder Injury and Treatment
- Tracheal and airway disorders
- Epigenetics and DNA Methylation
- Knee injuries and reconstruction techniques
- Periodontal Regeneration and Treatments
- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Shoulder and Clavicle Injuries
- Trauma Management and Diagnosis
- Bone Metabolism and Diseases
- Cancer-related molecular mechanisms research
- Tendon Structure and Treatment
- Bone fractures and treatments
- Cell Adhesion Molecules Research
- Sarcoma Diagnosis and Treatment
- Wound Healing and Treatments
- Adipose Tissue and Metabolism
- Bone health and treatments
China Medical University Hospital
2016-2025
China Medical University
2016-2025
China Medical University
2019-2024
Institute of Biomedical Sciences, Academia Sinica
2014-2024
International Drug Development
2017-2024
Development Center for Biotechnology
2024
National Medical Products Administration
2019-2023
National Yang Ming University Hospital
2010-2021
National Yang Ming Chiao Tung University
2012-2021
Amgen (United States)
2020
The Wharton's jelly of the umbilical cord contains mucoid connective tissue and fibroblast-like cells. Using flow cytometric analysis, we found that mesenchymal cells isolated from express matrix receptors (CD44, CD105) integrin markers (CD29, CD51) but not hematopoietic lineage (CD34, CD45). Interestingly, these also significant amounts stem cell (SH2, SH3). We therefore investigated potential to differentiate into cardiomyocytes by treating them with 5-azacytidine or culturing in...
CD133 (prominin-1), a 5-transmembrane glycoprotein, has recently been considered to be an important marker that represents the subset population of cancer stem-like cells. Herein we report isolation CD133-positive cells (LC-CD133+) and CD133-negative (LC-CD133−) from tissue samples ten patients with non-small cell lung (LC) five LC lines. LC-CD133+ displayed higher Oct-4 expressions ability self-renew may represent reservoir proliferative potential for generating Furthermore, LC-CD133+,...
Recent reports indicated that vascular remodeling and angiogenesis are promoted by conditioned medium from the cells referred to as multipotent stromal (MSCs). However, molecular events triggered MSC-conditioned (CdM) were not defined. We examined effects of CdM human MSCs on cultures primary aortic endothelial (HAECs). The inhibited hypoxia-induced apoptosis cell death HAECs. It also tube formation HAECs in an assay vitro. Conditioned incubated under hypoxic conditions serum-free basal for...
The ability of stem/progenitor cells to migrate and engraft into host tissues is key their potential use in gene cell therapy. Among the interest are adherent from bone marrow, referred as mesenchymal stem or multipotent stromal (MSC). Since marrow environment hypoxic, with oxygen tensions ranging 1% 7%, we decided test whether hypoxia can upregulate chemokine receptors enhance human MSCs vivo. Short-term exposure increased expression CX3CR1and CXCR4, both mRNA protein. After 1-day low...
Pulmonary fibrosis is characterized by fibroblast proliferation and extracellular matrix remodelling, leading to respiratory insufficiency. The mechanisms underlying this progressive devastating disease remain unclear. Conditions that can impair the function of endoplasmic reticulum (ER) cause accumulation unfolded or misfolded proteins, resulting in ER stress activation protein response (UPR). has been implicated many conditions including cancer, diabetes, obesity, inflammation. It also...
Tracheal implants using tissue-engineering and three-dimensional (3D) printing are promising, but challenges remain, including graft composition, anastomosis methods, tracheal tissue regeneration. This study examines the effectiveness of various materials in a large animal model. Thirty-eight surgeries were performed on porcine model, involving 2 cm circumferential excision with end-to-end anastomosis. The grafts used included translucent plastic material crystal (n=16), silicone (n=8),...
Bone marrow mesenchymal stem cells (MSCs) have the capacity for renewal and potential to differentiate into multiple lineages of tissues. In laboratory, MSCs tendency adhere culture dish plastic are characterized by fibroblastic morphology, but possess no specific markers select them. To isolate purify from bone marrow, we use a device-a comprising plate with 3-microm pores-to sieve out homogeneous population (termed size-sieved [SS] cells) aspirates. SS that adhered upper porous surface...
The aim of this study was to assess the efficacy human mesenchymal stem cells (hMSC) for targeting microscopic tumors and suicide gene or cytokine therapy. Immunodeficient mice were transplanted s.c. with colon cancer HT-29 Inv2 CCS line, 3 4 days later, i.v. "tracer" hMSCs expressing herpes simplex virus type 1 thymidine kinase (HSV1-TK) enhanced green fluorescent protein (EGFP) reporter genes. Subsequently, these examined specificity magnitude HSV1-TK(+), EGFP(+) cell engraftment...
Abstract Mesenchymal stem cells (MSCs) have the capacity for self‐renewal and can form bone, fat, cartilage. Alginate forms a viscous solution when dissolved in 0.9% saline gels on contact with divalent cations. The viability phenotype maintenance of chondrocytes alginate beads been well documented. However, little is known about effect microencapsulation chondrogenesis MSCs. In this study, human MSCs encapsulated were cultured serum‐free medium addition transforming growth factor (TGF)β1...
We investigated the effect of human umbilical mesenchymal stem cells (HUMSCs) from Wharton's jelly on carbon tetrachloride (CCl4)–induced liver fibrosis in rats. Rats were treated with CCl4 for 4 weeks, and this was followed by a direct injection HUMSCs into their livers. After more weeks treatment (8 all), rats HUMSC transplants [CCl4 (8W)+HUMSC liver] exhibited significant reduction fibrosis, as evidenced Sirius red staining collagen content assay, comparison 8 without (8W)]. Moreover,...
Recent studies have indicated that cancer stem-like cells (CSCs) exhibit a high resistance to current therapeutic strategies, including photodynamic therapy (PDT), leading the recurrence and progression of colorectal (CRC). In cancer, autophagy acts as both tumor suppressor promoter. However, role in CSCs PDT has not been reported. this study, were isolated from using PROM1/CD133 (prominin 1) expression, which is surface marker commonly found on stem various tissues. We demonstrated...
Background Bone loss induced by hypoxia is associated with various pathophysiological conditions, however, little known about the effects of and related signaling pathways on osteoblast differentiation bone formation. Because marrow-derived mesenchymal stem cells (MSCs) survive under hypoxic conditions readily differentiate into osteoblasts standard induction protocols, they are a good in vitro model to study differentiation. Methodology/Principle Findings Using human MSCs, we discovered...
Tumors are influenced by a microenvironment rich in inflammatory cytokines, growth factors and chemokines, which may promote tumor growth. Interleukin-6 (IL-6) is multifunctional cytokine known as regulator of immune inflammation responses. IL-6 has also been reported to be associated with progression chemoresistance different types cancers. In our study, we demonstrated that enriches the properties lung cancer stem-like cells A549 cultured spheroid medium. promotes sphere formation...
Abstract BACKGROUND: In the current study, authors sought to identify molecular mechanisms underlying chemoresistance of lung cancer stem or initiation cells (cancer cells). METHODS: A549 before and after selective enrichment a subpopulation were treated with superoxide traditional chemotherapeutics determine their sensitivity resistance these cytotoxic agents. Apoptotic activity was measured using variety fluorescence‐based biochemical techniques. Specific pathways involved in cell‐enriched...
Wound healing can be improved by transplanting mesenchymal stem cells (MSCs). In this study, we have demonstrated the benefits of conditioned medium derived from human MSCs (CM-MSC) in wound using an excisional model. CM-MSC accelerated closure with increased reepithelialization, cell infiltration, granulation formation, and angiogenesis. Notably, enhanced epithelial endothelial migration, suggesting contribution migration to CM-MSC. Cytokine array, ELISA analysis, quantitative RT-PCR...
Lineage commitment and tumorigenesis, traits distinguishing stem cells, have not been well characterized compared in mesenchymal cells derived from human dental pulp (DP-MSCs) bone marrow (BM-MSCs). Here, we report DP-MSCs exhibit increased osteogenic potential, possess decreased adipogenic form dentin pulp-like complexes, are resistant to oncogenic transformation when BM-MSCs. Genome-wide RNA-seq differential expression analysis reveal differences adipocyte osteoblast differentiation...
Hypoxia, a common environmental condition, influences cell signals and functions. Here, we compared the effects of hypoxia (1% oxygen) normoxia (air) on chondrogenic differentiation human mesenchymal stem cells (MSCs). For in vitro differentiation, MSCs were concentrated to form pellets subjected conditions appropriate for under hypoxia, followed by analysis expression genes proteins chondrogenesis endochondral ossification. induced increased chondrogenesis, but decreased ossification those...
The immunomodulatory effects of mesenchymal stem cells (MSCs) are established. However, the MSCs on neutrophil survival in acute lung injury (ALI) remain unclear. goal this study was to investigate effect an MSC-conditioned medium (MSC-CM) apoptosis endotoxin-induced ALI. In study, MSC-CM delivered via tail vein injection wild-type male C57BL/6 mice 4 h after intratracheal lipopolysaccharide (LPS). Twenty-four hours later, bronchoalveolar lavage fluid (BALF) and tissue were collected perform...