A5071132969- HIV Research and Treatment
- CAR-T cell therapy research
- HIV/AIDS drug development and treatment
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Cytomegalovirus and herpesvirus research
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Carbohydrate Chemistry and Synthesis
- Protein purification and stability
- Crystallization and Solubility Studies
- Biochemical and Molecular Research
- Cytokine Signaling Pathways and Interactions
- Drug Solubulity and Delivery Systems
- Complement system in diseases
University of Trento
2017-2023
University of Geneva
2013-2015
The San Raffaele Telethon Institute for Gene Therapy
2014
HIV-2 and SIVMAC are AIDS-causing, zoonotic lentiviruses that jumped to humans rhesus macaques, respectively, from SIVSM-bearing sooty mangabey monkeys. Cross-species transmission events such as these sometimes necessitate virus adaptation species-specific, host restriction factors TRIM5. Here, a new human activity is described blocks viruses of the SIVSM/SIVMAC/HIV-2 lineage. Human T, B, myeloid cell lines, peripheral blood mononuclear cells dendritic were 4 >100-fold less transducible by...
A vast portion of the mammalian genome is transcribed as long non-coding RNAs (lncRNAs) acting in cytoplasm with largely unknown functions. Surprisingly, lncRNAs have been shown to interact ribosomes, encode peptides, or act ribosome sponges. These functions still remain mostly undetected and understudied owing lack efficient tools for genome-wide simultaneous identification ribosome-associated peptide-producing lncRNAs. Here, we present AHA-mediated RIBOsome isolation (AHARIBO), a method...
SIVSM is a lentivirus endemic to the West African sooty mangabey (Cercocebus atys). HIV-2 and SIVMAC are zoonoses that resulted from transmission humans Asian rhesus macaques (Macaca mulatto), respectively. Human leukemia cell lines, human peripheral blood mononuclear cells CD4+ T cells, were 4 50-fold less permissive for than HIV-1. In contrast, transduction of adherent lines was equivalent Consistent with adaptation not restricted as potently SIVMAC. rescued up level HIV-1 by As2O3,...
The host transmembrane protein SERINC5 is incorporated into retrovirus particles and inhibits HIV-1 infectivity. lentiviral Nef counteracts by downregulating it from the cell surface preventing its incorporation virions. ability of to antagonize factor varies in magnitude between different isolates. After having identified a subtype H nef allele unable promote infectivity presence SERINC5, we investigated molecular determinants responsible for defective counteraction factor. Chimeric...
A poorly water-soluble drug (Efavirenz) was mechanically activated by ball-milling. The effect of the mechanical activation on dissolution behavior and bioavailability investigated revealing possible correlations with grinding action, in terms crystallinity, particle size morphology.With proper selection parameters kinetics can be controlled, both velocity as amount dissolved drug. In vitro biological tests show that milling does not impair ability Efavirenz to inhibit HIV-1 infection (p...
Abstract Most RNA footprinting approaches that require ribonuclease cleavage generate fragments bearing a phosphate or cyclic group at their 3′ end. Unfortunately, current library preparation protocols rely only on hydroxyl for adaptor ligation poly-A tailing. Here, we developed circAID-p-seq, PCR-free selective phospho-RNA sequencing. As proof of concept, applied circAID-p-seq to ribosome profiling, which is based sequencing protected by ribosomes after endonuclease digestion....
The battle between viruses and their host is armed by cellular defences, often referred to as restriction factors, on one side, the counteracting measures expressed viral genomes, other side. presence of cell-derived barriers which interfere with virus replication was first revealed for retroviruses in 70s. Since description FV1 (1), a cytoplasmic murine factor inhibiting MLV replication, several blocks that impair cell surface, cytoplasm, nucleus have later been discovered. latest addition...
SUMMARY A vast portion of the mammalian genome is transcribed as long non-coding RNAs (lncRNAs) acting in cytoplasm with largely unknown functions. Surprisingly, lncRNAs have been shown to interact ribosomes, encode uncharacterized proteins, or act ribosome sponges. These functions still remain mostly undetected and understudied owing lack efficient tools for genome-wide simultaneous identification ribosome-associated peptide-producing lncRNAs. Here we present AHARIBO, a method detection...
Abstract Accurate positional information concerning ribosomes and RNA binding proteins with respect to their transcripts is important understand the global regulatory network underlying protein fate in living cells. Most footprinting approaches generate fragments bearing a phosphate or cyclic groups at 3′ end. Unfortunately, all current protocols for library preparation rely only on presence of hydroxyl group. Here, we developed circAID-p-seq, PCR-free phospho-RNA sequencing. We applied...