A5012883477- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Virus-based gene therapy research
- Research on Leishmaniasis Studies
- interferon and immune responses
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- Carbon dioxide utilization in catalysis
- Trypanosoma species research and implications
- Herpesvirus Infections and Treatments
- MicroRNA in disease regulation
- COVID-19 diagnosis using AI
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Axial and Atropisomeric Chirality Synthesis
- HIV/AIDS Research and Interventions
- Monoclonal and Polyclonal Antibodies Research
- Covalent Organic Framework Applications
Indian Institute of Science Education and Research, Bhopal
2017-2024
National Centre for Cell Science
2024
University of Trento
2015-2023
Indian Institute of Science Education and Research Berhampur
2020
Indian Institute of Science Education and Research Mohali
2020
Indian Institute of Science Education and Research Kolkata
2020
Indian Institute of Science Education and Research, Tirupati
2020
Indian Institute of Science Education and Research Pune
2020
Advanced Centre for Treatment, Research and Education in Cancer
2011-2016
International Centre for Genetic Engineering and Biotechnology
2015
Background Squamous cell lung carcinomas account for approximately 25% of new carcinoma cases and 40,000 deaths per year in the United States. Although there are multiple genomically targeted therapies adenocarcinoma, none has yet been reported squamous carcinoma. Methodology/Principal Findings Using SNP array analysis, we found that a region chromosome segment 8p11-12 containing three genes–WHSC1L1, LETM2, FGFR1–is amplified 3% adenocarcinomas 21% carcinomas. Furthermore, demonstrated...
The host proteins, SERINC3 and SERINC5, have been recently shown to incorporate into HIV-1 particles compromise their ability fuse with target cells, an effect that is antagonized by the viral Nef protein. Envelope (Env) glycoproteins from different isolates exhibit a broad range of sensitivity SERINC-mediated restriction, mechanism which SERINCs interfere fusion remains unclear. Here, we show incorporation SERINC5 virions in absence inhibits formation small pores between viruses cells....
An unprecedented worldwide spread of the SARS-CoV-2 has imposed severe challenges on healthcare facilities and medical infrastructure. The global research community faces urgent calls for development rapid diagnostic tools, effective treatment protocols, most importantly, vaccines against pathogen. Pooling together expertise across broad domains to innovate solutions is need hour. With these requirements in mind, this review, we provide detailed critical accounts leading efforts at...
Abstract To reside and multiply successfully within the host macrophages, Leishmania parasites impair generation of reactive oxygen species (ROS), which are a major defense mechanism against any invading pathogen. Mitochondrial uncoupling proteins associated with mitochondrial ROS generation, is contributor total cellular generation. In present study we have demonstrated that donovani infection strong upregulation protein 2 (UCP2), negative regulator located at inner membrane mitochondria....
TLRs, which form an interface between mammalian host and microbe, play a key role in pathogen recognition initiation of proinflammatory response thus stimulating antimicrobial activity survival. However, certain intracellular pathogens such as Leishmania can successfully manipulate the TLR signaling, hijacking defensive strategies host. Despite presence lipophosphoglycan, TLR2 ligand capable eliciting host-defensive cytokine response, on surface Leishmania, adopted by parasite to silence...
The lentivirus equine infectious anemia virus (EIAV) encodes the small protein S2, a pathogenic determinant that is important for replication and disease progression in horses. No molecular function had been linked to this accessory protein. We report S2 can replace activity of Negative factor (Nef) HIV-1 infectivity, being required antagonize inhibitory Serine incorporator (SERINC) proteins on Nef-defective HIV-1. Like Nef, excludes SERINC5 from particles requires an ExxxLL motif predicted...
We have demonstrated the catalytic CO<sub>2</sub> conversion, and antibacterial antiviral activity, including against HIV-1, of zinc oxide-infused ionic porous organic frameworks.
Breakthrough infections by emerging SARS-CoV-2 variants raise significant concerns. Here, we sequence-characterized the spike gene from breakthrough that corresponded to B.1.617 sublineage. Delineating functional impact of mutations revealed N-terminal domain (NTD)-specific E156G/Δ157-158 contributed increased infectivity and reduced sensitivity vaccine-induced antibodies. A six-nucleotide deletion (467-472) in spike-coding region introduced this change NTD. We confirmed presence cases...
The functional consequences of circular RNA (circRNA) expression on HIV-1 replication are largely unknown. Using a customized protocol involving direct nanopore sequencing, here, we captured circRNAs from HIV-1-infected T cells and identified ciTRAN, circRNA that modulates transcription. We found infection induces ciTRAN in Vpr-dependent manner interacts with SRSF1, protein known to repress Our results suggest hijacks exclude serine/arginine-rich splicing factor 1 (SRSF1) the viral...
CD40 plays dual immunoregulatory roles in Leishmania major infection and tumor regression. The functional duality emerges from CD40-induced reciprocal p38MAPK ERK-1/2 phosphorylations. Because phosphotyrosine-based signaling hematopoietic cells is regulated by the phosphotyrosine phosphatase SHP-1, which not implied signaling, we examined whether SHP-1 played any anti-leishmanial function. We observed that a weaker stimulation increased activation. inhibition or overexpression inhibited An...
BACKGROUND: Limited data are available on the prevalence and prognostic significance of human papillomavirus (HPV) in squamous cell carcinoma head neck (SCCHN) Indian population. AIM: The present study aimed to determine HPV p16 an cohort SCCHN assess their correlation influence tobacco use patient outcomes. MATERIALS AND METHODS: status 170 patients treated with curative chemoradiotherapy was determined using immunohistochemistry polymerase chain reaction, respectively, further correlated...
Precise regulation of innate immunity is crucial for development appropriate host against microbial infections and maintenance immune homeostasis. MicroRNAs are small non-coding RNAs, post-transcriptional regulator multiple genes, act as a rheostat protein expression. Here, we identified microRNA-30e-5p induced by hepatitis B virus other viruses that master immunity. Moreover, pegylated interferons treatment patients with HBV viral reduction also reduces miRNA. Additionally, have shown the...
Intramacrophage pathogen Leishmania donovani escapes host immune response by subverting Toll-like receptor (TLR) signaling, which is critically regulated protein ubiquitination. In the present study, we identified tumor necrosis factor receptor-associated (TRAF) 3, degradative ubiquitination of essential for TLR4 activation, as a target to deactivate LPS-mediated signaling. We used LPS-treated RAW 264.7 cells and compared TLR4-mediated in these with L. + LPS costimulated macrophages. TRAF3,...
Reported is the first organocatalytic asymmetric 1,3-alkyl shift in alkyl aryl ethers for synthesis of chiral 3,3'-diaryloxindoles using a Brønsted acid catalyst. Preliminary results showed that each enantiomer 3,3'-diaryloxindole, and racemic mixture, different antiproliferative activities against HeLa cell lines by an MTT assay.
Abstract Upon infection, Mycobacterium tuberculosis (Mtb) deploys specialized secretion machinery to deliver virulent proteins with the capacity modulate a variety of host-cellular pathways. Studies on identification intra-macrophage Mtb proteins, however, are constricted by an inability selectively enrich these effectors against overwhelming protein content host. Here, we introduce Mtb-selective labeling method based genetic incorporation azidonorleucine (Anl) through expression mutant...
The establishment of SARS CoV-2 spike-pseudotyped lentiviral (LV) systems has enabled the rapid identification entry inhibitors and neutralizing agents, alongside allowing for study this emerging pathogen in BSL-2 level facilities. While such frameworks recapitulate cellular process ACE2+ cells, they are largely unable to factor supplemental contributions by other genes. To address this, we performed an unbiased ORF screen identified nucleoprotein (N) as a potent enhancer LV particle...
HIV-1 antagonizes SERINC5 by redundant mechanisms, primarily through Nef and additionally via envelope glycoprotein. Paradoxically, preserves function to ensure the exclusion of from virion incorporation regardless availability that can confer resistance, suggesting additional roles virion-incorporated host factor. Here, we report an unusual mode action in inhibiting viral gene expression. This inhibition is observed only myeloid lineage cells but not epithelial or lymphoid origin. We found...
Depending on the strength of signal dose, CD40 receptor (CD40) controls ERK-1/2 and p38MAPK activation. At low is maximally phosphorylated but minimally phosphorylated; as dose increases, phosphorylation reduced whereas reciprocally enhanced. The mechanism reciprocal activation these two MAPKs remains un-elucidated. Here, our computational model, coupled to experimental perturbations, shows that observed reciprocity a system-level behavior an assembly kinases arranged in modules....