The release of large quantities microorganisms to soil for purposes such as pest control or plant growth promotion may affect the indigenous microbial communities. In our study, we investigated potential effects Metarhizium brunneum ART2825 on fungi and prokaryota in bulk using high-throughput sequencing ribosomal markers. Different formulations this strain, combinations fungus with garlic efficacy-enhancing agent, were tested over 4 months a pot field experiment carried out biological...

Soil microbial diversity has major influences on ecosystem functions and services. However, due to its complexity uneven distribution of abundant rare taxa, quantification soil remains challenging thereby impeding integration into long-term monitoring programs. Using metabarcoding, we analyzed bacterial fungal communities at 30 sites from the three land-use types arable land, permanent grassland, forest with a yearly sampling between snowmelt first fertilization over five years. Unlike...

Soilborne diseases pose a significant threat to crop production and their control remains major challenge. Compost is valuable soil amendment when it comes suppressing soilborne pathogens, but the effectiveness of different composts varies cannot yet be predicted. Microbial communities in compost play key role disease suppression, therefore microbial taxa may serve as indicators for suppressive composts. In this study, we investigated 37 from seven commercial producers analyze association...

Efficacy of the Metarhizium brunneum Petch (Hypocreales: Clavicipitaceae) strain ART2825 for control wireworms (Agriotes obscurus (L.), Coleoptera: Elateridae) was examined in a semi-field pot experiment. Pots were treated late summer during sowing spring oat as cover crop. Survival assessed four weeks after their release October 2013, and 30 April 2014. Viability persistence fungus determined by counting colony forming units from substrate samples microsatellite analyses recovered isolates....

Cross-species transferability of 41 previously published simple sequence repeat (SSR) markers was assessed for 11 species the entomopathogenic fungus Metarhizium. A collection 65 Metarhizium strains including all 54 used in a recent phylogenetic revision genus were characterized. Between 15 and 34 polymorphic SSR produced scorable PCR amplicons seven species, M. anisopliae, brunneum, guizhouense, lepidiotae, majus, pingshaense, robertsii. To provide genotyping tools concurrent analysis these...

Mountain areas harbor large climatic and geographic gradients form numerous habitats that promote high overall biodiversity. Compared to macroorganisms, knowledge about drivers of biodiversity distribution soil bacteria in mountain regions is still scarce but a prerequisite for conservation bacterial functions soils. An important question is, whether communities with similar structures share environmental preferences. Using metabarcoding the 16S rRNA gene marker, we assessed at 255 sites...

The fungal species Metarhizium pingshaense, M. anisopliae, robertsii, and brunneum, a monophyletic group informally referred to as the PARB complex, are well known facultative entomopathogens, including many commercialized strains used for biological pest control. Accurate expedient identification of isolates represents an important first step when addressing ecological application-related questions involving these fungi. To this end, species-specific multiplexed polymerase chain reaction...

Species of the fungal genus Metarhizium are globally distributed pathogens arthropods, and a number biological control products based on these fungi have been commercialized to variety pest arthropods. In this study, we investigate abundance population structure spp. in three land-use types—arable land, grassland, forest—to provide detailed information habitat selection factors that drive occurrence soil. At 10 sites each type, which all part Swiss national soil-monitoring network (NABO),...

ABSTRACT Compost applications vary in their plant growth promotion and disease suppression, likely due to differences physico-chemical biological parameters. Our hypothesis was that bacteria are important for suppression of composts and, therefore, having these traits would contain similar sets indicative bacterial taxa. Seventeen prepared from five different commercial providers starting materials were classified accordingly with bioassays using cress plants the pathogen Pythium ultimum....

Entomopathogenic fungi are used for biological control of insect pests. Metarhizium brunneum Petch (Hypocreales) has potential to Diabrotica virgifera LeConte (Chrysomelidae), which is a major pest maize in North America and recently invaded Europe. The inundative application an entomopathogenic fungal strain results high densities propagules the soil can potentially affect microbial communities their multiple functions soil. objective present study was assess effects M. on prokaryotic pot...

Inundative mass application of Metarhizium brunneum BIPESCO 5 (Hypocreales, Clavicipitaceae) is used for the biological control Diabrotica v. virgifera (Coleoptera, Chrysomelidae). Long-term field trials were performed in three Austrian maize fields—with different cultivation techniques and infestation rates—in order to evaluate efficacy treatment pest larvae. In addition, indigenous spp. population structure was assessed compare sites with application. Annual product Granmet-PTM...

Abstract Soil microbial diversity has major influences on ecosystem functions and services. However, due to its complexity uneven distribution of abundant rare taxa, quantification soil remains challenging thereby impeding integration into long-term monitoring programs. Using metabarcoding, we analyzed bacterial fungal communities over five years at thirty sites from the three land-use types, arable land, permanent grassland, forest. Unlike biomass alpha-diversity, community compositions...

Amplicon sequencing allows for simultaneous assessment of inoculation success and responses indigenous communities after microbial inoculation. However, the presence a highly dominant taxon may bias correct due to dilution effects, chimera formation, or preferential amplification. Here, we present simple test assess these biases.

Boden ist ein Habitat, das eine grosse Vielfalt an Mikroorganismen, d. h. Bakterien, Archaeen, Pilzen und Protisten, beherbergt. Wir wissen, dass diese Kleinstlebewesen für Vielzahl spezifischer Bodenfunktionen verantwortlich sind. Im Rahmen des Biodiversitätsmonitorings der Schweiz haben wir 255 Standorten die Diversität Bodenbakterien -pilze untersucht. Insgesamt wurden 109 693 Bakterien- 28 085 Pilztaxa gefunden, wobei etwa 2/3 1/3 einer Gattung Rest nur höheren taxonomischen Klasse...