The recalcitrant nature of lignocellulosic biomass hinders efficient exploitation this fraction for energy production. A better understanding the microorganisms able to convert plant-based feedstocks is needed improve anaerobic digestion biomass. In study, active thermophilic cellulose-degrading were identified from a full-scale digester fed with maize by using metagenome-resolved protein stable isotope probing (protein-SIP). 13C-cellulose was converted into 13C-methane 13/12C ratio 0.127...

Hydrothermal liquefaction (HTL) shows promise for converting wet biomass waste into biofuel, but the resulting high-strength process water (PW) requires treatment. This study explored enhancing energy recovery by anaerobic digestion using semi-batch reactors. Co-digesting manure with HTL-PW from wheat straw-manure co-HTL yielded methane (43-49% of chemical oxygen demand, COD) at concentrations up to 17.8 gCOD·L

PBAT (poly butylene adipate-co-terephthalate) is a widely used biodegradable plastic, but the knowledge about its metabolization in anaerobic environments very limited. In this study, digester sludge from municipal wastewater treatment plant was as inoculum to investigate biodegradability of monomers thermophilic conditions. The research employs combination 13C-labelled and proteogenomics track labelled carbon identify microorganisms involved. A total 122 peptides interest were identified...

Aspergillus niger is an opportunistic pathogen commonly found in a variety of indoor and outdoor environments. An environmental isolate A. from pig farm was resistant to itraconazole, in-depth investigations were conducted better understand cellular responses that occur during growth when this exposed antifungal. Using combination cultivation techniques, antibiotic stress testing, label-free proteomics, study investigated the physiological metabolic sublethal levels antifungal stress....

Biogas production from organic waste is a waste-to-energy technology with the potential to contribute significantly sustainable energy production. Upgrading of biogas using in situ biomethanation hydrogen has for surplus electricity storage, and delivery methane content >90%, allowing easier integration into natural gas grid, as well conversion other products. Microbial communities reactors undergo changes, however, these changes are largely unexplored. In present study, metagenome-resolved...

Most bacteria live in microbial assemblages like biofilms and granules, each layer of these provides a niche for certain with specific metabolic functions. In this study, gentle (non-destructive) extraction approach based on cation exchange resin defined shear was employed to gradually disintegrate biomass collect single layers aerobic granules from full-scale municipal wastewater treatment plant. The community composition granule characterized using next-generation sequencing (NGS)...

A full-scale membrane bioreactor (MBR) with ultrafiltration, followed by granular activated carbon (GAC), was examined to determine the potential of reusing treated water as a source drinking or for irrigation. The major part bacteria removal took place in MBR, whereas GAC removed substantial amounts organic micropollutants. Annual variations inflow and infiltration resulted concentrated influent during summer diluted winter. E. coli high throughout process (average log 5.8), effluent...

Azo dye reduction by syntrophic microbial communities is still unclear, with conflicting observations reported in the literature. In this study, biodegradation mechanism of a model azo Lactococcus lactis strain LLSP-01 isolated from an acidogenic reactor system was investigated. Proteomics and RT-PCR analysis results showed that azoreductase (AzoL) not expressed isolate during exposure to Direct Black 22. contrast, appears involve biosorption glycoconjugates, particularly exopolysaccharides...

Multiple stage anaerobic system was found to be an effective strategy for reductive decolorization of azo dyes in the presence sulfate. Bulk color removal (56-90%) achieved concomitant with acidogenic activity 1st-stage reactor (R1), while organic matter (≤100%) and sulfate reduction occurred predominantly 2nd-stage (R2). However, dye mechanism metabolic routes involved remain unclear. The microbial communities conditions affecting a two-stage digestion (AD) were elucidated using amplicon...

Methicillin-resistant Staphylococcus aureus (MRSA) is a major human pathogen that causes wide range of infections. Its resistance to β-lactam antibiotics complicates treatment due the limited number with activity against MRSA. To investigate development alternative therapeutics, mechanisms mediate antibiotic in MRSA need be fully understood. In this study, cells were subjected stress from methicillin combination three cannabinoid compounds and analyzed using proteomics assess changes...

This paper offers characteristic formula constructions in the real-time logic Lν for several behavioural relations between (states of) timed automata. The studied this work are (bi)similarity, ready simulation, faster-than bisimilarity and trace inclusion. formulae delivered by our have size which is linear that of automaton they logically describe. also applies to bisimulation equivalence, an exponential space construction was previously offered Laroussinie, Larsen Weise.

Antibiotic resistance in fungal isolates is increasing on a global scale. Despite knowledge that pig farmers are occupationally exposed to infectious species of fungi, such as Aspergillus spp., little known regarding their potential exposure antifungal-resistant spp. The aim this study obtain the antifungal profiles taken from different source materials—including airborne dust, surface faeces, and straw—within farm. EUCAST broth microdilution method was used for testing 43 sampled 3 periods...

Tetrabromobisphenol A (TBBPA) is the most used flame retardant worldwide and has become a threat to aquatic ecosystems. Previous research into degradation of this micropollutant in anaerobic bioreactors suggested several identities putative TBBPA degraders. However, organisms actively degrading under situ conditions have so far not been identified. Protein-stable isotope probing (protein-SIP) cutting-edge technique microbial ecology for enabling link between identity function conditions....

Manure management is a significant source of global methane emissions, and there an increased interest in understanding predicting emissions. The hydrolysis rate manure organic matter critical for We estimated constants crude protein, fibers, lipids used the Arrhenius equation to describe its dependency on temperature. Simultaneously, measurements emission, 13/12 C isotope ratios, methanogen community were conducted. This was achieved by incubating fresh pig without inoculum at 10°C, 15°C,...

The biogas produced through anaerobic digestion (AD) of renewable feedstocks is one the promising alternatives to replace fossil-derived energy. Even though lignocellulosic biomass most abundant on earth, only a small fraction being used towards resources recovery, leaving great potential unexploited. In this study, combination state-of-art genomic techniques and engineered systems were further advance knowledge production from lignocellulosic-rich residues microbiome involved in hereof. A...

This paper offers characteristic formula constructions in the real-<br />time logic L for several behavioural relations between (states of)<br />timed automata. The studied this work are<br (bi)similarity, timed ready simulation, faster-than bisimilarity<br />and trace inclusion. formulae delivered by<br />our have size which is linear that of <br />automaton they logically describe. also applies to characteristic<br />formula bisimulation equivalence,...

<title>Abstract</title> <bold>Background</bold>Presence of carbohydrates hampers protein degradation in anaerobic digesters. To understand this phenomenon, we used proteogenomics to identify the active protein-degraders presence low and high concentrations. Active metabolic pathways identified were investigated using proteomics with¹³C-protein substrates (protein stable isotope probing).<bold>Results</bold><italic>Acinetobacter</italic>was as main under both protein-fed...